2. Others
  3. Fluorescent Dye
  4. Cypate

Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging.

For research use only. We do not sell to patients.

Cypate Chemical Structure

Cypate Chemical Structure

CAS No. : 95837-47-1

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  
Synthetic products have potential research and development risk.

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Other In-stock Forms of Cypate:

Other Forms of Cypate:

Cypate Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging[1][2].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Plated 1×106 of each cell type on individual 35-mm glass-bottom petri dishes.
2.After 24 hours, the cell lines are incubated for 2 hours with a 10 µM solution of Cypate in phenol red free medium.
3.After treatment, the cells are either washed 3× with PBS and fixed with 4% paraformaldehyde for imaging or has their intracellular Cypate contents collected for spectroscopy and/or LC-MS analysis.
4.Confocal microscopy is performed on in vitro cell culture experiments. Cypate is excited with 647 nm wavelength[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cypate Related Antibodies
In Vivo

Cypate (10 nmol; IV; every 24 hours for 6 days) causes the liver to have the highest accumulation[1].
The fluorescence signal of Cypate (5 mg/kg; IV) in the tumor of mice (Balb/c mice with 4T1 cells) is weak and quickly decayed, possibly due to fast elimination of free cypate from body. Stronger fluorescence in liver and kidney is observed in free cypate group. The Cypate fluorescence signal is assessed using an IVIS Lumina imaging system (Ex-745 nm; Em-800 nm)[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Foxn1nu/Foxn1nu nude 2.5-month-old female mice with breast cancer cells (MDA-MB-231 Luc2)[1]
Dosage: 10 nmol in 100 µL PBS
Administration: IV; every 24 hours for 6 days
Result: The accumulation alone in the tumor was negligible for 24 hours after the injection.
The liver had the highest accumulation at all time points.
Molecular Weight

624.77

Formula

C41H40N2O4
CAS No.
SMILES

O=C(CC[N+]1=C(/C=C/C=C/C=C/C=C2N(CCC(O)=O)C3=C(C/2(C)C)C4=C(C=CC=C4)C=C3)C(C)(C)C5=C1C=CC6=C5C=CC=C6)[O-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

Cypate Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Cypate95837-47-1Fluorescent DyeCyaninedyenear infraredNIRvivo tumor imagingA549Inhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Cypate
Cat. No.:
HY-D1719
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

Request for HNMR Report

We have received your request and will respond to you as soon as possible.