Cypate
Based on 1 publication(s) in Google Scholar
Cypate, a cyanine dye, is a near infrared (NIR) fluorescent probe for in vivo tumor imaging.
For research use only. We do not sell to patients.
- Purity: 98.60%
- CAS No.: 95837-47-1
- Formula: C41H40N2O4
- Molecular Weight:624.77
-
Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
-
Biological Activity
-
Chemical Information
- Purity & Documentation
- References
-
Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cypate
More
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1.Plated 1×106 of each cell type on individual 35-mm glass-bottom petri dishes.
2.After 24 hours, the cell lines are incubated for 2 hours with a 10 μM solution of Cypate in phenol red free medium.
3.After treatment, the cells are either washed 3× with PBS and fixed with 4% paraformaldehyde for imaging or has their intracellular Cypate contents collected for spectroscopy and/or LC-MS analysis.
4.Confocal microscopy is performed on in vitro cell culture experiments. Cypate is excited with 647 nm wavelength[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
The fluorescence signal of Cypate (5 mg/kg; IV) in the tumor of mice (Balb/c mice with 4T1 cells) is weak and quickly decayed, possibly due to fast elimination of free cypate from body. Stronger fluorescence in liver and kidney is observed in free cypate group. The Cypate fluorescence signal is assessed using an IVIS Lumina imaging system (Ex-745 nm; Em-800 nm)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Foxn1nu/Foxn1nu nude 2.5-month-old female mice with breast cancer cells (MDA-MB-231 Luc2)[1]
-
Dosage:10 nmol in 100 µL PBS
-
Administration:IV; every 24 hours for 6 days
-
Result:The accumulation alone in the tumor was negligible for 24 hours after the injection.
The liver had the highest accumulation at all time points.
Chemical Information
-
CAS No. 95837-47-1
-
Appearance Solid
-
Molecular Weight 624.77
-
Formula C41H40N2O4
-
Color Green to dark green
-
SMILES
O=C(CC[N+]1=C(/C=C/C=C/C=C/C=C2N(CCC(O)=O)C3=C(C/2(C)C)C4=C(C=CC=C4)C=C3)C(C)(C)C5=C1C=CC6=C5C=CC=C6)[O-]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (1)
-
Journal Impact Factor
-
Most Recent
-
Mater Today Bio
An exosome-biomimetic photothermal nanocarrier for IGF2BP2 siRNA delivery and enhanced ferroptosis in head and neck squamous cell carcinoma. [Abstract]2026 Mar 5:37:102999. PMID: 41859644
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Mona Doshi, et al. Cypate and Cypate-Glucosamine as Near-Infrared Fluorescent Probes for In Vivo Tumor Imaging. Mol Pharmacol. 2019 May;95(5):475-489. [Content Brief]
[2]. Yuanyuan Li, et al. Light-Decomposable Polymeric Micelles with Hypoxia-Enhanced Phototherapeutic Efficacy for Combating Metastatic Breast Cancer. Pharmaceutics. 2022 Jan 21;14(2):253. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz