Cypate hydrochloride 

Cypate is a near-infrared fluorescent dye that belongs to the family of photosensitizers. Cypate has high photostability and optical properties, and is often used in near-infrared optical imaging, as well as optical imaging, tumor marking, and drug delivery. In addition, Cypate is also used as a molecular probe and combined with targeting molecules (such as CBT or small interfering RNA) to achieve efficient detection and imaging of specific cells or tissues^[1][2].

In the study, Cypate coupled upconversion nanoparticles (UCNP-cy) loaded with small interfering RNA gene with anti-heat shock protein 70 (UCNP-cy-siRNA) resulted in targeted cell damage and anti-tumor effects^[1].
Cypate can also be coupled to CBT (Cysteine-containing Peptide Backbone Tag), which can be used to track, label and image specific biomolecules or cells. For example, Cypate-CBT, as a near-infrared photoacoustic (PA) probe, can be used to specifically image and track cathepsin B (CTSB) activity in CTSB-overexpressing cells and tumors in real time. After Cypate-CBT entered CTSB overexpressing cells, it underwent glutathione reduction and CTSB cleavage to generate cypate nanoparticles Cypate-CBT-NPs, which enhanced the intensity and retention time of PA signal at tumor sites. Cypate-CBT has the potential to be used as an effective PA imaging agent for clinical diagnosis of early cancer^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

661.23

C₄₁H₄₁ClN₂O₄

Solid

Green to dark green

O=C(CC[N+]1=C(/C=C/C=C/C=C/C=C2N(CCC(O)=O)C3=C(C/2(C)C)C4=C(C=CC=C4)C=C3)C(C)(C)C5=C1C=CC6=C5C=CC=C6)[O-].[H]Cl

Room temperature in continental US; may vary elsewhere.

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Wang L, et al. Cypate-conjugated porous upconversion nanocomposites for programmed delivery of heat shock protein 70 small interfering RNA for gene silencing and photothermal ablation[J]. Advanced Functional Materials, 2016, 26(20): 3480-3489.

  [2]. Wang C, et al. Cathespin B-Initiated Cypate Nanoparticle Formation for Tumor Photoacoustic Imaging. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202114766.  [Content Brief]