2. Anti-infection
  3. Antibiotic Bacterial
  4. Demeclocycline

Demeclocycline is an orally active tetracycline antibiotic. Demeclocycline impairs protein synthesis by binding to the 30S ribosomal subunit to inhibit binding of aminoacyl tRNA. Demeclocycline shows anti-bacterial activitise to a wide variety of bacterial infections.

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Demeclocycline Chemical Structure

Demeclocycline Chemical Structure

CAS No. : 127-33-3

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Based on 4 publication(s) in Google Scholar

Other Forms of Demeclocycline:

Demeclocycline Related Antibodies

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Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Demeclocycline is an orally active tetracycline antibiotic. Demeclocycline impairs protein synthesis by binding to the 30S ribosomal subunit to inhibit binding of aminoacyl tRNA. Demeclocycline shows anti-bacterial activitise to a wide variety of bacterial infections[1][2].

IC50 & Target

Tetracycline

 

In Vitro

Demeclocycline (0-100 μM; 24 h) treatment reduces AQP2 abundance in mpkCCD cells[3].
Demeclocycline (10 μM; 24 h) treatment promotes the activity of monocytes and macrophages[4].
Demeclocycline (1-10 μM; 72 h) treatment directly affects the growth of brain tumorinitiating cells[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Demeclocycline Related Antibodies

Western Blot Analysis[3]

Cell Line: MpkCCD cells
Concentration: 0-100 μM
Incubation Time: 24 hours
Result: Decreased AQP2 abundance in mpkCCD cells, with significant effects at 50 μM.

Cell Viability Assay[4]

Cell Line: mouse bone marrow derived macrophages and monocytes
Concentration: 10 μM
Incubation Time: 24 hours
Result: Enhanced TNF-α production and modulated monocyte functions.

Cell Viability Assay[4]

Cell Line: brain tumorinitiating cells
Concentration: 1, 5, and 10 μM
Incubation Time: 72 hours
Result: Inhibited cells growth in two ways: using monocytes as an intermediary, and directly by affecting the proliferation and sphere-forming capacity of brain tumorinitiating cells.
In Vivo

Demeclocycline (Intraperitoneal injection; 40 mg/kg; once daily; 48 h) treatment results in a significant reduction of hyponatremia and a significant correction of the hypoosmolality, and is not nephrotoxic[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Wistar rats induced with hyponatremia[3]
Dosage: 40 mg/kg
Administration: Intraperitoneal injection; 40 mg/kg; once daily; 48 hours
Result: Increased urine volume, decreased urine osmolality, and caused a significantly increased fractional excretion of water.
Animal Model: Male Wistar rats induced with hyponatremia[3]
Dosage: 40 mg/kg
Administration: Intraperitoneal injection; 40 mg/kg; once daily; 48 hours
Result: Indicated the effect in the renal inner medulla for AQP2 and AC5/6 specifically, and not secondary toxicity effect.
Clinical Trial
Molecular Weight

464.85

Formula

C21H21ClN2O8
CAS No.
Appearance

Solid

Color

Green to dark green

SMILES

O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@H](O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4Cl)N
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

H2O : 40 mg/mL (86.05 mM; Need ultrasonic)

DMSO : 25 mg/mL (53.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1512 mL 10.7562 mL 21.5123 mL
5 mM 0.4302 mL 2.1512 mL 4.3025 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.69 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 2.1512 mL 10.7562 mL 21.5123 mL 53.7808 mL
5 mM 0.4302 mL 2.1512 mL 4.3025 mL 10.7562 mL
10 mM 0.2151 mL 1.0756 mL 2.1512 mL 5.3781 mL
15 mM 0.1434 mL 0.7171 mL 1.4342 mL 3.5854 mL
20 mM 0.1076 mL 0.5378 mL 1.0756 mL 2.6890 mL
25 mM 0.0860 mL 0.4302 mL 0.8605 mL 2.1512 mL
30 mM 0.0717 mL 0.3585 mL 0.7171 mL 1.7927 mL
40 mM 0.0538 mL 0.2689 mL 0.5378 mL 1.3445 mL
50 mM 0.0430 mL 0.2151 mL 0.4302 mL 1.0756 mL
H2O 60 mM 0.0359 mL 0.1793 mL 0.3585 mL 0.8963 mL
80 mM 0.0269 mL 0.1345 mL 0.2689 mL 0.6723 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Demeclocycline Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Demeclocycline127-33-3AntibioticBacterialanti-bacterial activityMpkCCDmacrophagesmonocytesbrain tumorinitiating cellsInhibitorinhibitorinhibit

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