Demeclocycline hydrochloride
Based on 5 publication(s) in Google Scholar
Demeclocycline hydrochloride is an orally active tetracycline antibiotic that inhibits the binding of aminoacyl tRNA by binding to the 30S ribosomal subunit, thereby affecting protein synthesis. Demeclocycline hydrochloride exhibits antibacterial activity against a broad spectrum of bacterial infections.
For research use only. We do not sell to patients.
- Purity: 95.15%
- CAS No.: 64-73-3
- Formula: C21H22Cl2N2O8
- Molecular Weight:501.31
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Storage:
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Publications Citing Use of MedChemExpress (MCE) Demeclocycline hydrochloride
MoreAll Antibiotic Isoforms
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Biological Activity
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Tetracycline |
Demeclocycline (0-100 μM; 24 h) hydrochloride treatment reduces AQP2 abundance in mpkCCD cells[3].
Demeclocycline (10 μM; 24 h) hydrochloride treatment promotes the activity of monocytes and macrophages[4].
Demeclocycline (1-10 μM; 72 h) hydrochloride treatment directly affects the growth of brain tumorinitiating cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MpkCCD cells
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Concentration:0-100 μM
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Incubation Time:24 hours
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Result:Decreased AQP2 abundance in mpkCCD cells, with significant effects at 50 μM.
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Cell Line:Mouse bone marrow derived macrophages and monocytes
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Concentration:10 μM
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Incubation Time:24 hours
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Result:Enhanced TNF-α production and modulated monocyte functions.
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Cell Line:Brain tumorinitiating cells
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Concentration:1, 5, and 10 μM
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Incubation Time:72 hours
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Result:Inhibited cells growth in two ways: using monocytes as an intermediary, and directly by affecting the proliferation and sphere-forming capacity of brain tumorinitiating cells.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Wistar rats induced with hyponatremia[3]
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Dosage:40 mg/kg
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Administration:Intraperitoneal injection; 40 mg/kg; once daily; 48 hours
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Result:Increased urine volume, decreased urine osmolality, and caused a significantly increased fractional excretion of water.
Indicated the effect in the renal inner medulla for AQP2 and AC5/6 specifically, and not secondary toxicity effect.
| NCT Number | Sponsor | Condition | Start Date |
Phase
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|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 64-73-3
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Appearance Solid
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Molecular Weight 501.31
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Formula C21H22Cl2N2O8
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Color Light yellow to khaki
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SMILES
O=C(C(C1=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@]3([H])[C@H](O)C4=C(C(C3=C(O)[C@@]21O)=O)C(O)=CC=C4Cl)N.Cl
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Structure Classification
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Initial Source
Streptococcus aureofaciens
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (5)
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Journal Impact Factor
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Most Recent
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Mol Syst Biol
2022 Sep;18(9):e11081. PMID: 36065847 -
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Chemosphere
Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]2019 Jun:225:378-387. PMID: 30884299
Solvent & Solubility
DMSO : 100 mg/mL (199.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 33.33 mg/mL (66.49 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.99 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (4.99 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (284 KB)
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SDS (557 KB)
- English - EN (557 KB)
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- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
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Handling Instructions (2659 KB)
References
[1]. I Chopra, et al. Tetracyclines, molecular and clinical aspects. J Antimicrob Chemother. 1992 Mar;29(3):245-77. [Content Brief]
[2]. D Schnappinger, et al. Tetracyclines: antibiotic action, uptake, and resistance mechanisms. Arch Microbiol. 1996 Jun;165(6):359-69. [Content Brief]
[3]. Marleen L A Kortenoeven, et al. Demeclocycline attenuates hyponatremia by reducing aquaporin-2 expression in the renal inner medulla. Am J Physiol Renal Physiol. 2013 Dec 15;305(12):F1705-18. [Content Brief]
[4]. Susobhan Sarkar, et al. Demeclocycline Reduces the Growth of Human Brain Tumor-Initiating Cells: Direct Activity and Through Monocytes. Front Immunol. 2020 Feb 21;11:272. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| H2O / DMSO | 1 mM | 1.9948 mL | 9.9739 mL | 19.9477 mL | 49.8693 mL |
| 5 mM | 0.3990 mL | 1.9948 mL | 3.9895 mL | 9.9739 mL | |
| 10 mM | 0.1995 mL | 0.9974 mL | 1.9948 mL | 4.9869 mL | |
| 15 mM | 0.1330 mL | 0.6649 mL | 1.3298 mL | 3.3246 mL | |
| 20 mM | 0.0997 mL | 0.4987 mL | 0.9974 mL | 2.4935 mL | |
| 25 mM | 0.0798 mL | 0.3990 mL | 0.7979 mL | 1.9948 mL | |
| 30 mM | 0.0665 mL | 0.3325 mL | 0.6649 mL | 1.6623 mL | |
| 40 mM | 0.0499 mL | 0.2493 mL | 0.4987 mL | 1.2467 mL | |
| 50 mM | 0.0399 mL | 0.1995 mL | 0.3990 mL | 0.9974 mL | |
| 60 mM | 0.0332 mL | 0.1662 mL | 0.3325 mL | 0.8312 mL | |
| DMSO | 80 mM | 0.0249 mL | 0.1247 mL | 0.2493 mL | 0.6234 mL |
| 100 mM | 0.0199 mL | 0.0997 mL | 0.1995 mL | 0.4987 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Demeclocycline hydrochloride Related Classifications
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