1. Anti-infection
  2. Bacterial
    Antibiotic
  3. Methacycline hydrochloride

Methacycline hydrochloride 

Cat. No.: HY-B0449 Purity: 99.53%
Handling Instructions

Methacycline hydrochloride is a tetracycline antibiotic and can inhibits bacterial protein synthesis. Methacycline hydrochloride is a potent epithelial-mesenchymal transition (EMT) inhibitor. Methacycline hydrochloride blocks EMT in vitro and fibrogenesis in vivo without directly affecting TGF-β1 Smad signaling. Methacycline hydrochloride is an antimicrobial and has the potential for pulmonary fibrosis.

For research use only. We do not sell to patients.

Methacycline hydrochloride Chemical Structure

Methacycline hydrochloride Chemical Structure

CAS No. : 3963-95-9

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Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
200 mg USD 90 In-stock
Estimated Time of Arrival: December 31
500 mg USD 180 In-stock
Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

Methacycline hydrochloride is a tetracycline antibiotic and can inhibits bacterial protein synthesis. Methacycline hydrochloride is a potent epithelial-mesenchymal transition (EMT) inhibitor. Methacycline hydrochloride blocks EMT in vitro and fibrogenesis in vivo without directly affecting TGF-β1 Smad signaling. Methacycline hydrochloride is an antimicrobial and has the potential for pulmonary fibrosis[1].

In Vitro

Methacycline hydrochloride is an inhibitor of A549 EMT with an IC50 of roughly 5 μM[1].
In vitro, Methacycline hydrochloride (10, 20 μM; for 48 hours) inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline hydrochloride inhibits TGF-β1–induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

In vivo, Methacycline hydrochloride (100 mg/kg/day; ip; beginning 10 days after intratracheal Bleomycin) improves survival at Day 17. Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA, ARE attenuated by Methacycline hydrochloride (Day 17)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

478.88

Formula

C₂₂H₂₃ClN₂O₈

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (104.41 mM; Need ultrasonic)

H2O : 6.67 mg/mL (13.93 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.0882 mL 10.4410 mL 20.8821 mL
5 mM 0.4176 mL 2.0882 mL 4.1764 mL
10 mM 0.2088 mL 1.0441 mL 2.0882 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (5.22 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
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Methacycline hydrochloride
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HY-B0449
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