2. Anti-infection
  3. Bacterial Antibiotic
  4. Amikacin

Amikacin (BAY 41-6551) is a semisynthetic kanamycin analog that is active against most Gram-negative bacteria, including gentamicin- and tobramycin-resistant strains. Significant inhibitory effect. Amikacin is ototoxic and nephrotoxic. Amikacin can be used in bacteriostatic, anti-cancer and analgesic studies.

For research use only. We do not sell to patients.

CAS No. : 37517-28-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
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Customer Review

Based on 26 publication(s) in Google Scholar

Other Forms of Amikacin:

Amikacin Related Antibodies

Top Publications Citing Use of Products
Microbiological Assay

    Amikacin purchased from MedChemExpress. Usage Cited in: AMB Express. 2024 Dec 24;14(1):141.  [Abstract]

    Combinational antimicrobial effects between PVB and conventional antibiotics against MRSA ATCC 43,300. TET, Tetracycline. DOX, Doxycycline. E, Erythromycin. AZI, Azithromycin. P, Penicillin. CAZ, Ceftazidime. AMP, Ampicillin. CEZ, Cefazolin. CEF, Cefotaxime. OXA, Oxacillin. CRO, Ceftriaxone. AMK, Amikacin. GEN, Gentamycin, KANA, Kanamycin. TOB, Tobramycin. SPC, Spectinomycin.

    View All Antibiotic Isoform Specific Products:

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    Aminoglycoside Glycopeptide Lipopeptide Macrolide Oxazolidinone Quinolone Tetracycline β-lactam

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Amikacin (BAY 41-6551) is a semisynthetic kanamycin analog that is active against most Gram-negative bacteria, including gentamicin- and tobramycin-resistant strains. Significant inhibitory effect. Amikacin is ototoxic and nephrotoxic. Amikacin can be used in bacteriostatic, anti-cancer and analgesic studies[1][2][3][4][5].

    IC50 & Target

    Aminoglycoside

     

    TXNIP

     

    Cellular Effect
    Cell Line Type Value Description References
    BMDM CC50
    > 90 μg/mL
    Compound: AMK
    Cytotoxicity against mouse BMDM cells
    Cytotoxicity against mouse BMDM cells
    		[PMID: 35307568]
    Vero IC50
    > 6.25 μg/mL
    Compound: Amikacin
    Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay
    Cytotoxicity against monkey Vero cells assessed as decrease in cell viability after 72 hrs by MTT assay
    		[PMID: 28552337]
    Vero IC50
    > 62.5 μg/mL
    Compound: Amikacin
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    Cytotoxicity against african green monkey Vero cells after 72 hrs by MTT assay
    		[PMID: 23352268]
    In Vitro

    Amikacin (30 µg, 0-24 h) has antibacterial activity, with a MIC50 value of 512 µg/mL against clinically isolated E. coli, and has a synergistic effect with imipenem (HY-B1369A), and the antibacterial effect is better when used in combination[1].
    Amikacin (250 µg/mL, 0-24 h) inhibits the migration and invasion of human breast cancer cell line MDA-MB-231 cells by up-regulating the expression of TXNIP, indicating its anti-tumor potential[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Amikacin Related Antibodies

    Western Blot Analysis[2]

    Cell Line: human breast cancer cell line MDA-MB-231 cells
    Concentration: 250 µg/mL
    Incubation Time: 0-24 h
    Result: Upregulated the expression of TXNIP to 4.87 times.
    In Vivo

    Amikacin (single 30 mg/kg, s.c. or i.p.) has an analgesic effect in mice and has a synergistic effect when combined with morphine, but the analgesic effect of Amikacin can be reversed by Naloxone (HY-17417A)[3].
    Amikacin (500 mg/kg/day for 8 days, s.c.) damages calpain activity in rat cochlea, promotes the degradation of sensory cells and neurons, and then leads to ototoxicity[4].
    Amikacin (100 and 500 mg/kg/day for 10 days, s.c.) is nephrotoxic and its continued accumulation in rats can lead to kidney damage[5].

    Pharmacokinetic Analysis in SD rats[5]

    Route Dose (mg/kg) Ka (h-1) Ke1 (h-1) t1/2 (h) V (liter/kg) AUC0-∞ (mg·h/mL)
    s.c. 100 1.20 6.77 0.10 0.28 53.0
    s.c. 500 1.40 1.39 0.50 0.55 649.7

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    585.60

    Formula

    C22H43N5O13
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O[C@@H]1[C@H]([C@@H](C[C@H](N)[C@H]1O[C@@]([C@@H]([C@@H](O)[C@@H]2O)O)([H])O[C@@H]2CN)NC([C@@H](O)CCN)=O)O[C@@]([C@@H]([C@@H](N)[C@@H]3O)O)([H])O[C@@H]3CO
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    H2O : 100 mg/mL (170.77 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7077 mL 8.5383 mL 17.0765 mL
    5 mM 0.3415 mL 1.7077 mL 3.4153 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.93%

    SDS (393 KB)

    COA (256 KB) HNMR (289 KB) RP-HPLC (146 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.7077 mL 8.5383 mL 17.0765 mL 42.6913 mL
    5 mM 0.3415 mL 1.7077 mL 3.4153 mL 8.5383 mL
    10 mM 0.1708 mL 0.8538 mL 1.7077 mL 4.2691 mL
    15 mM 0.1138 mL 0.5692 mL 1.1384 mL 2.8461 mL
    20 mM 0.0854 mL 0.4269 mL 0.8538 mL 2.1346 mL
    25 mM 0.0683 mL 0.3415 mL 0.6831 mL 1.7077 mL
    30 mM 0.0569 mL 0.2846 mL 0.5692 mL 1.4230 mL
    40 mM 0.0427 mL 0.2135 mL 0.4269 mL 1.0673 mL
    50 mM 0.0342 mL 0.1708 mL 0.3415 mL 0.8538 mL
    60 mM 0.0285 mL 0.1423 mL 0.2846 mL 0.7115 mL
    80 mM 0.0213 mL 0.1067 mL 0.2135 mL 0.5336 mL
    100 mM 0.0171 mL 0.0854 mL 0.1708 mL 0.4269 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Amikacin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Amikacin37517-28-5BAY 41-6551BacterialAntibioticAntibacterialCell migrationCell InvasionE. coliMDA-MB-231 cellOtotoxicityNephrotoxicityInhibitorinhibitorinhibit

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