Melimine

Melimine exhibits no independent antiviral activity against H1N1, MHV-1 or HSV-1; when combined with RK610, it produces an additive effect against H1N1 in MDCK cells and against MHV-1 in A9 cells, while showing no interaction against HSV-1 in Vero cells^[1].
Melimine exhibits high cytotoxicity against A9 cells at a concentration of 62.5 μM, and retains cytotoxicity when combined with 1.09 μM RK610; at a concentration of 31.25 μM, either used alone or in combination with 1 μM RK610, it shows no cytotoxicity against MDCK cells^[1].
Melimine acetate‑modified soft contact lenses increase the adhesion inhibition rate of Pseudomonas aeruginosa ATCC 9027 and Staphylococcus aureus ATCC 6538 to 80%, and exhibit no cytotoxicity to mammalian ocular cells^[2].

Melimine inhibits and kills the growth of Pseudomonas aeruginosa strains 6206, 6294, Paer1 and ATCC 19660, with MIC values ranging from 66 to 132 nM and MBC values ranging from 132 to 528 nM^[3].
Melimine dose-dependently neutralizes LPS of Pseudomonas aeruginosa, with a neutralization rate of 71% at 1×10⁰ MIC and 82% at 2×10⁰ MIC^[3].
Melimine (66-132 nM) rapidly depolarizes the cytoplasmic membrane of Pseudomonas aeruginosa 6294 within 30 seconds; it permeabilizes the cytoplasmic membrane of Pseudomonas aeruginosa 6294, allowing Sytox Green to start entering the cells at 5 minutes; it induces concentration-dependent release of DNA/RNA from Pseudomonas aeruginosa 6294; and it lyses Pseudomonas aeruginosa 6294 in a concentration-dependent manner^[3].
Melimine (66 nM; 30-150 min) permeabilizes the cell membrane of Pseudomonas aeruginosa strain 6294 in 74.8% of cells within 30 minutes^[3].
Melimine (66-132 nM; 2-10 min) releases 75% of total cellular ATP at 66 nM and 92% of total cellular ATP at 132 nM from Pseudomonas aeruginosa 6294 within 2 minutes, while the viable cell count decreases by >3.0 log₁₀^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.