EGFR-IN-63
EGFR-IN-63 is an EGFR inhibition (IC50: 0.096 μM) and it has anticancer activity in MCF-7 cells (IC50: 2.49 μM).
For research use only. We do not sell to patients.
- CAS No.: 2414635-72-4
- Formula: C20H12BrN5S
- Molecular Weight:434.31
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
In Vitro
EGFR- IN-63 (2-4 hours) has superior EGFR inhibition (IC50 = 0.096 μM) compared to Gefitinib (IC50 = 0.166 μM)and anticancer activity against MCF-7 cell line (IC50 = 2.49 μM) compared to Gefitinib (IC50 = 4.972 μM). EGFR- IN-63 leads to pre G1 apoptosis with cell growth arrest at G2/M phase in MCF-7 cells after 24 h, the percentage of DNA content (41.45 %) shows increase compared to the control cells (7.1 %). EGFR- IN-63 arrest cell growth at G2/M phase and induces its apoptotic effect. The percentage of the total apoptotic cells in MCF-7 cell line increases after treatment with EGFR- IN-63(24.19 %) relative to control cells (1.47 %)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:A549, MCF-7,WI38, PC9 and HCC827 cell lines
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Concentration:10% of the culture medium volume
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Incubation Time:2 - 4 hours
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Result:Displayed superior EGFR inhibitory、anticancer activity and low cytotoxicity.
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Cell Line:MCF-7
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Concentration:0.01 μM
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Incubation Time:24 hour
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Result:Led to pre G1 apoptosis with cell growth arrest at G2/M phase in MCF-7 cells.
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Cell Line:MCF-7
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Concentration:0.01 μM
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Incubation Time:24 hour
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Result:Revealed cell growth arrest at G2/M phase and inducted apoptotic effect.
Chemical Information
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CAS No. 2414635-72-4
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Molecular Weight 434.31
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Formula C20H12BrN5S
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SMILES
BrC1=CC2=C(NC3=NC4=CC=CC=C4S3)N=C(C5=CC=CN=C5)N=C2C=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
EGFR-IN-63 Related Classifications
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