Cat. No.: HY-136429

Ethylhydrocupreine

(Synonyms: Optochin)

Customer Review

Based on 1 Customer Validation

Ethylhydrocupreine (Optochin) is a quinine derivate with antimicrobial activity against S. pneumoniae. Ethylhydrocupreine also possesses antimalarial activity against Plasmodium falciparum, with an IC₅₀ of 25.75 nM. Ethylhydrocupreine is a Gallus gallus taste 2 receptors (ggTas2r1, ggTas2r2 and ggTas2r7) agonist.

For research use only. We do not sell to patients.

Purity: 99.64%
CAS No.: 522-60-1
Formula: C₂₁H₂₈N₂O₂
Molecular Weight:340.46

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Ethylhydrocupreine Chemical Structure

CAS No.: 522-60-1

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

S. pneumoniae^[1]
IC50: 25.75 nM (Plasmodium falciparum)^[3]
ggTas2r1, ggTas2r2 and ggTas2r7^[4]

All Parasite Isoforms :

Amebae

Coccidia

Leishmania

Mite

Plasmodium

Toxoplasma

Trypanosoma

Schistosome

Cellular Effect

Cell Line

Type Value Description References

HepG2 CC₅₀
>100 μM
Compound: 1

Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue assay

Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue assay
[PMID: 30779564]

Huh-7 CC₅₀
53.82 μM
Compound: GNF-Pf-5532

NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)

NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)
[PMID: 18579783]

In Vitro

The mutation rate to Ethylhydrocupreine (Optochin) resistance is estimated using fluctuation analysis in three capsulated S. pneumoniae strains (S. pneumoniae D39 NCTC 7466, S. pneumoniae R6 ATCC BAA-255 and S. pneumoniae ATCC 49619). The exposure to subinhibitory concentrations of penicillin increased the mutation rate (expressed as mutation per cell division) to Ethylhydrocupreine (Optochin) resistance between 2.1- and 3.1-fold for all three strains studied^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The injection of 1 cc. of a 24 hour dextrose blood broth culture of virulent Type I pneumococci into the right pleural cavity of guinea pigs produces acute suppurative pleuritis on both sides associated with suppurative pericarditis. The injection of 1 cc. of 1:500 solutions of Ethylhydrocupreine hydrochloride into each pleural cavity of guinea pigs at varying intervals up to 24 hours after pleural infection has usually shown a marked curative influence. Similar results are observed with dogs^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Line	Type	Value	Description	References
HepG2	CC₅₀	>100 μM Compound: 1	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability after 48 hrs by Alamar Blue assay	[PMID: 30779564]
Huh-7	CC₅₀	53.82 μM Compound: GNF-Pf-5532	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]

Chemical Information

CAS No. 522-60-1

Appearance Solid

Molecular Weight 340.46

Formula C₂₁H₂₈N₂O₂

Color White to off-white

SMILES

O[C@@H]([C@@](C[C@@H]1CC2)([H])[N@@]2C[C@@H]1CC)C3=CC=NC(C=C4)=C3C=C4OCC

Synonyms

Optochin

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder -20°C 3 years

In solvent -80°C 6 months

-20°C 1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (293.72 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.9372 mL

14.6860 mL

29.3720 mL

5 mM

0.5874 mL

2.9372 mL

5.8744 mL

10 mM

0.2937 mL

1.4686 mL

2.9372 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:

To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.34 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg

Animal Weight (Per Animal) Animal Weight (Per Animal)
g

Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL

Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

%
+

+
%
Tween-80 + +

%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact
MedChemExpress (MCE) .

Tel: 609-228-6898
E-mail: [email protected]
Technical Support: [email protected]

Method for preparing in vivo working solution for animal experiments:
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Saline .

Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (287 KB)

SDS (392 KB)

English - EN (392 KB)

Français - FR (392 KB)

Deutsch - DE (392 KB)

Norwegian - NO (392 KB)

Español - ES (392 KB)

Swedish - SV (392 KB)

Italian - IT (392 KB)

Korean - KR (392 KB)

Portuguese - PT (392 KB)

COA (2659 KB)

Elemental Analysis Report (2659 KB)

HNMR (2659 KB)

CNMR (2659 KB)

RP-HPLC (2659 KB)

NP-HPLC (2659 KB)

MS (2659 KB)

LCMS (2659 KB)

IR (2659 KB)

GC (2659 KB)

OR (2659 KB)

SDS-PAGE (2659 KB)

SEC-HPLC (2659 KB)

Other Report (2659 KB)

Handling Instructions (2659 KB)

References

[1]. J A Kolmer, et al. CHEMOTHERAPEUTIC STUDIES WITH ETHYLHYDROCUPREINE HYDROCHLORIDE IN EXPERIMENTAL PNEUMOCOCCUS PLEURITIS. J Exp Med. 1921 May 31;33(6):693-711. [Content Brief]

[2]. Paulo R Cortes, et al. Subinhibitory Concentrations of Penicillin Increase the Mutation Rate to Optochin Resistance in Streptococcus Pneumoniae. J Antimicrob Chemother. 2008 Nov;62(5):973-7. [Content Brief]

[3]. Nassira Mahmoudi, et al. Identification of New Antimalarial Drugs by Linear Discriminant Analysis and Topological Virtual Screening. J Antimicrob Chemother. 2006 Mar;57(3):489-97. [Content Brief]

[4]. Antonella Di Pizio, et al. Molecular Features Underlying Selectivity in Chicken Bitter Taste Receptors. Front Mol Biosci. 2018 Jan 31;5:6. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg

DMSO 1 mM 2.9372 mL 14.6860 mL 29.3720 mL 73.4301 mL

5 mM 0.5874 mL 2.9372 mL 5.8744 mL 14.6860 mL

10 mM 0.2937 mL 1.4686 mL 2.9372 mL 7.3430 mL

15 mM 0.1958 mL 0.9791 mL 1.9581 mL 4.8953 mL

20 mM 0.1469 mL 0.7343 mL 1.4686 mL 3.6715 mL

25 mM 0.1175 mL 0.5874 mL 1.1749 mL 2.9372 mL

30 mM 0.0979 mL 0.4895 mL 0.9791 mL 2.4477 mL

40 mM 0.0734 mL 0.3672 mL 0.7343 mL 1.8358 mL

50 mM 0.0587 mL 0.2937 mL 0.5874 mL 1.4686 mL

60 mM 0.0490 mL 0.2448 mL 0.4895 mL 1.2238 mL

80 mM 0.0367 mL 0.1836 mL 0.3672 mL 0.9179 mL

100 mM 0.0294 mL 0.1469 mL 0.2937 mL 0.7343 mL

Ethylhydrocupreine Related Classifications

HY-136429 Related Classifications

Anti-infection

Bacterial Parasite

Disease Areas

Infection

Bacterial Infection Parasitic Infection

Infection

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Keywords

Ethylhydrocupreine
522-60-1
Optochin
Bacterial
Parasite
Antimicrobial
bactericidal
bitter
taste
ggTas2r1
ggTas2r2
ggTas2r7
antimalarial
S. pneumoniae
Inhibitor
inhibitor
inhibit

Your Recently Viewed Products

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.9372 mL	14.6860 mL	29.3720 mL	73.4301 mL
5 mM	0.5874 mL	2.9372 mL	5.8744 mL	14.6860 mL
10 mM	0.2937 mL	1.4686 mL	2.9372 mL	7.3430 mL
15 mM	0.1958 mL	0.9791 mL	1.9581 mL	4.8953 mL
20 mM	0.1469 mL	0.7343 mL	1.4686 mL	3.6715 mL
25 mM	0.1175 mL	0.5874 mL	1.1749 mL	2.9372 mL
30 mM	0.0979 mL	0.4895 mL	0.9791 mL	2.4477 mL
40 mM	0.0734 mL	0.3672 mL	0.7343 mL	1.8358 mL
50 mM	0.0587 mL	0.2937 mL	0.5874 mL	1.4686 mL
60 mM	0.0490 mL	0.2448 mL	0.4895 mL	1.2238 mL
80 mM	0.0367 mL	0.1836 mL	0.3672 mL	0.9179 mL
100 mM	0.0294 mL	0.1469 mL	0.2937 mL	0.7343 mL

Ethylhydrocupreine

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Ethylhydrocupreine

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All Parasite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table