USP20 - ubiquitin specific peptidase 20 Gene

Also Known as VDU2; hVDU2; LSFR3A

Species: Homo sapiens

Gene Type: protein coding

Gene ID: 10868

About USP20

Cytogenetic location: 9q34.11 Genomic coordinates (GRCh38): 9:129,835,458-129,881,828 (from NCBI)

This gene has 9 transcripts (splice variants), 207 orthologues and 71 paralogues. Ubiquitous expression in lymph node (RPKM 8.9), bone marrow (RPKM 8.3) and 25 other tissues.

Summary

This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin Ligase complex. In addition to being ubiquitinated by the VHL-E3 Ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 Adrenergic Receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]

USP20 Products (3)

mRNA	Protein	Name
NM_001008563.5	NP_001008563.2	ubiquitin carboxyl-terminal hydrolase 20
NM_001110303.4	NP_001103773.2	ubiquitin carboxyl-terminal hydrolase 20
NM_006676.8	NP_006667.3	ubiquitin carboxyl-terminal hydrolase 20

Gene Ontology

Molecular Function
Biological Process
Cellular Component

Molecular Function GO Annotation	Evidence	References	Source
enables G protein-coupled receptor binding	IPI IPI: Inferred from physical interaction	19424180	GOA
enables cysteine-type deubiquitinase activity	IDA IDA: Inferred from direct assay	19424180	GOA
enables cysteine-type endopeptidase activity	IMP IMP: Inferred from mutant phenotype	19424180	GOA
enables protein binding	IPI IPI: Inferred from physical interaction	19615732	GOA

Biological Process GO Annotation	Evidence	References	Source
involved in antiviral innate immune response	IDA IDA: Inferred from direct assay	27801882	GOA
involved in negative regulation of canonical NF-kappaB signal transduction	IDA IDA: Inferred from direct assay	26839314	GOA
involved in positive regulation of autophagy	IDA IDA: Inferred from direct assay	29487085	GOA
involved in protein K48-linked deubiquitination	IDA IDA: Inferred from direct assay	19424180	GOA
involved in protein K63-linked deubiquitination	IDA IDA: Inferred from direct assay	19424180	GOA
involved in protein deubiquitination	IDA IDA: Inferred from direct assay	19424180	GOA
involved in regulation of G protein-coupled receptor signaling pathway	IMP IMP: Inferred from mutant phenotype	19424180	GOA

Cellular Component GO Annotation	Evidence	References	Source
located in centrosome	IDA IDA: Inferred from direct assay	23486064	GOA

EXP: Inferred from Experiment IDA: Inferred from direct assay IPI: Inferred from physical interaction IMP: Inferred from mutant phenotype IGI: Inferred from genetic interaction IEP: Inferred from expression pattern

USP20 Protein Structure

zf-UBP

UCH

DUSP

0
200
400
600
800
914 a.a.

Protein Preferred Names	Protein Names
ubiquitin carboxyl-terminal hydrolase 20	deubiquitinating enzyme 20

USP20 Protein-protein interaction Information

Type	Protein Name	Protein ID	Interactor	Interactor Species	Interactor ID	Detection Method	References
Intra	USP20	Q9Y2K6	MAGEA11	Homo sapiens	P43364-2	Y2H Prey Pooling	25416956
Intra	USP20	Q9Y2K6	MAGEA11	Homo sapiens	P43364-2	Y2H Array	25416956
Intra	USP20	Q9Y2K6	VAPB	Homo sapiens	Q53XM7	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	BEND7	Homo sapiens	Q8N7W2-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	BEND7	Homo sapiens	Q8N7W2-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	BEND7	Homo sapiens	Q8N7W2-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	BEND7	Homo sapiens	Q8N7W2-2	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	ZNF343	Homo sapiens	Q6P1L6	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZNF343	Homo sapiens	Q6P1L6	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZNF343	Homo sapiens	Q6P1L6	Y2H Array	32296183
Intra	USP20	Q9Y2K6	PBX4	Homo sapiens	Q9BYU1	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	PBX4	Homo sapiens	Q9BYU1	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	PBX4	Homo sapiens	Q9BYU1	Y2H Array	32296183
Intra	USP20	Q9Y2K6	CDK2AP1	Homo sapiens	O14519	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	CDK2AP1	Homo sapiens	O14519	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	CDK2AP1	Homo sapiens	O14519	Y2H Array	32296183
Intra	USP20	Q9Y2K6	VAPA	Homo sapiens	Q9P0L0	Anti Tag CoIP	19615732
Intra	USP20	Q9Y2K6	VAPA	Homo sapiens	Q9P0L0	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	KLHL20	Homo sapiens	Q9BS75	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	KLHL20	Homo sapiens	Q9BS75	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	KLHL20	Homo sapiens	Q9BS75	Y2H Array	32296183
Intra	USP20	Q9Y2K6	GPRASP3	Homo sapiens	Q6PI77	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	GPRASP3	Homo sapiens	Q6PI77	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	GPRASP3	Homo sapiens	Q6PI77	Y2H Array	32296183
Intra	USP20	Q9Y2K6	VAPB	Homo sapiens	O95292	Anti Tag CoIP	19615732
Intra	USP20	Q9Y2K6	VAPB	Homo sapiens	O95292	Y2H Array	31515488
Intra	USP20	Q9Y2K6	VAPB	Homo sapiens	O95292	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	CTAG1A	Homo sapiens	P78358	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	CTAG1A	Homo sapiens	P78358	Y2H Array	32296183
Intra	USP20	Q9Y2K6	CTAG1A	Homo sapiens	P78358	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	MCCD1	Homo sapiens	P59942	Y2H Array	32296183
Intra	USP20	Q9Y2K6	MCCD1	Homo sapiens	P59942	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZBTB10	Homo sapiens	Q96DT7-3	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZBTB10	Homo sapiens	Q96DT7-3	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZBTB10	Homo sapiens	Q96DT7-3	Y2H Array	32296183
Intra	USP20	Q9Y2K6	CCNL2	Homo sapiens	Q96S94-5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	CCNL2	Homo sapiens	Q96S94-5	Y2H Array	32296183
Intra	USP20	Q9Y2K6	CCNL2	Homo sapiens	Q96S94-5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	BRPF1	Homo sapiens	P55201-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	BRPF1	Homo sapiens	P55201-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	BRPF1	Homo sapiens	P55201-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	APBA2	Homo sapiens	Q99767-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	APBA2	Homo sapiens	Q99767-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	APBA2	Homo sapiens	Q99767-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	VAX1	Homo sapiens	Q5SQQ9-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	VAX1	Homo sapiens	Q5SQQ9-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	VAX1	Homo sapiens	Q5SQQ9-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	MBD3L1	Homo sapiens	Q8WWY6	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	MBD3L1	Homo sapiens	Q8WWY6	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	MBD3L1	Homo sapiens	Q8WWY6	Y2H Array	32296183
Intra	USP20	Q9Y2K6	RSPO4	Homo sapiens	Q2I0M5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	RSPO4	Homo sapiens	Q2I0M5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	RSPO4	Homo sapiens	Q2I0M5	Y2H Array	32296183
Intra	USP20	Q9Y2K6	SAMD12	Homo sapiens	Q8N8I0	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	SAMD12	Homo sapiens	Q8N8I0	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	SAMD12	Homo sapiens	Q8N8I0	Y2H Array	32296183
Intra	USP20	Q9Y2K6	HAND2	Homo sapiens	P61296-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	HAND2	Homo sapiens	P61296-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	HAND2	Homo sapiens	P61296-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	MEOX2	Homo sapiens	Q6FHY5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	MEOX2	Homo sapiens	Q6FHY5	Y2H Array	32296183
Intra	USP20	Q9Y2K6	MEOX2	Homo sapiens	Q6FHY5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZXDB	Homo sapiens	P98169	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZXDB	Homo sapiens	P98169	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZXDB	Homo sapiens	P98169	Y2H Array	32296183
Intra	USP20	Q9Y2K6	SRGAP2B	Homo sapiens	A0A286YEY3	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	SRGAP2B	Homo sapiens	A0A286YEY3	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	SRGAP2B	Homo sapiens	A0A286YEY3	Y2H Array	32296183
Intra	USP20	Q9Y2K6	FTO	Homo sapiens	Q9C0B1-2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	FTO	Homo sapiens	Q9C0B1-2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	FTO	Homo sapiens	Q9C0B1-2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	C1QTNF2	Homo sapiens	Q9BXJ5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	C1QTNF2	Homo sapiens	Q9BXJ5	Y2H Array	32296183
Intra	USP20	Q9Y2K6	C1QTNF2	Homo sapiens	Q9BXJ5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZNF408	Homo sapiens	Q9H9D4	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZNF408	Homo sapiens	Q9H9D4	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZNF408	Homo sapiens	Q9H9D4	Y2H Array	32296183
Intra	USP20	Q9Y2K6	ATP6V1G2	Homo sapiens	O95670	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ATP6V1G2	Homo sapiens	O95670	Y2H Array	32296183
Intra	USP20	Q9Y2K6	ATP6V1G2	Homo sapiens	O95670	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ALAS1	Homo sapiens	P13196	Y2H Array	25416956
Intra	USP20	Q9Y2K6	ZNF620	Homo sapiens	Q6ZNG0	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZNF620	Homo sapiens	Q6ZNG0	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZNF620	Homo sapiens	Q6ZNG0	Y2H Array	32296183
Intra	USP20	Q9Y2K6	EPAS1	Homo sapiens	Q99814	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	EPAS1	Homo sapiens	Q99814	Y2H Array	32296183
Intra	USP20	Q9Y2K6	EPAS1	Homo sapiens	Q99814	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	GPRASP2	Homo sapiens	Q96D09	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	GPRASP2	Homo sapiens	Q96D09	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	GPRASP2	Homo sapiens	Q96D09	Y2H Array	32296183
Intra	USP20	Q9Y2K6	TRIB3	Homo sapiens	Q96RU7	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	TRIB3	Homo sapiens	Q96RU7	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	TRIB3	Homo sapiens	Q96RU7	Y2H Array	32296183
Intra	USP20	Q9Y2K6	HSF2BP	Homo sapiens	O75031	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	HSF2BP	Homo sapiens	O75031	Y2H Array	32296183
Intra	USP20	Q9Y2K6	HSF2BP	Homo sapiens	O75031	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ITGB3BP	Homo sapiens	Q13352	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ITGB3BP	Homo sapiens	Q13352	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ITGB3BP	Homo sapiens	Q13352	Y2H Array	32296183
Intra	USP20	Q9Y2K6	AGR2	Homo sapiens	O95994	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	AGR2	Homo sapiens	O95994	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	AGR2	Homo sapiens	O95994	Y2H Array	32296183
Intra	USP20	Q9Y2K6	MRPL28	Homo sapiens	Q13084	Y2H Array	32296183
Intra	USP20	Q9Y2K6	MRPL28	Homo sapiens	Q13084	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	MRPL28	Homo sapiens	Q13084	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	MDFI	Homo sapiens	Q99750	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	MDFI	Homo sapiens	Q99750	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	MDFI	Homo sapiens	Q99750	Y2H Array	32296183
Intra	USP20	Q9Y2K6	MDFI	Homo sapiens	Q99750	Y2H Prey Pooling	25416956
Intra	USP20	Q9Y2K6	MDFI	Homo sapiens	Q99750	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	BEND5	Homo sapiens	Q7L4P6	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	TRIM23	Homo sapiens	P36406	Y2H Array	25416956
Intra	USP20	Q9Y2K6	TRIM23	Homo sapiens	P36406	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	SPRY2	Homo sapiens	O43597	Y2H Array	25416956
Intra	USP20	Q9Y2K6	FATE1	Homo sapiens	Q969F0	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	FATE1	Homo sapiens	Q969F0	Y2H Array	25416956
Intra	USP20	Q9Y2K6	FATE1	Homo sapiens	Q969F0	Y2H Array	32296183
Intra	USP20	Q9Y2K6	FATE1	Homo sapiens	Q969F0	Y2H Prey Pooling	25416956
Intra	USP20	Q9Y2K6	FATE1	Homo sapiens	Q969F0	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ENKD1	Homo sapiens	Q9H0I2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	ENKD1	Homo sapiens	Q9H0I2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ENKD1	Homo sapiens	Q9H0I2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	TNNI1	Homo sapiens	P19237	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	TNNI1	Homo sapiens	P19237	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	TNNI1	Homo sapiens	P19237	Y2H Array	32296183
Intra	USP20	Q9Y2K6	IHO1	Homo sapiens	Q8IYA8	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	IHO1	Homo sapiens	Q8IYA8	Y2H Array	32296183
Intra	USP20	Q9Y2K6	IHO1	Homo sapiens	Q8IYA8	Validated Y2H	25416956
Intra	USP20	Q9Y2K6	IHO1	Homo sapiens	Q8IYA8	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZFYVE26	Homo sapiens	Q68DK2-5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ZFYVE26	Homo sapiens	Q68DK2-5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ZFYVE26	Homo sapiens	Q68DK2-5	Y2H Array	32296183
Intra	USP20	Q9Y2K6	TTC23L	Homo sapiens	Q6PF05	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	TTC23L	Homo sapiens	Q6PF05	Y2H Array	32296183
Intra	USP20	Q9Y2K6	TTC23L	Homo sapiens	Q6PF05	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	TTC19	Homo sapiens	Q6DKK2	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	TTC19	Homo sapiens	Q6DKK2	Y2H Array	32296183
Intra	USP20	Q9Y2K6	TTC19	Homo sapiens	Q6DKK2	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ARID5A	Homo sapiens	Q03989	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	ARID5A	Homo sapiens	Q03989	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ARID5A	Homo sapiens	Q03989	Y2H Array	32296183
Intra	USP20	Q9Y2K6	ASCL1	Homo sapiens	P50553	Y2H Array	32296183
Intra	USP20	Q9Y2K6	ASCL1	Homo sapiens	P50553	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	ASCL1	Homo sapiens	P50553	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	IRAK1BP1	Homo sapiens	Q5VVH5	Y2H Prey Pooling	32296183
Intra	USP20	Q9Y2K6	IRAK1BP1	Homo sapiens	Q5VVH5	Validated Y2H	32296183
Intra	USP20	Q9Y2K6	IRAK1BP1	Homo sapiens	Q5VVH5	Y2H Array	32296183

Cross: Cross-species interaction Intra: Intraspecies interaction

Related Diseases

Diseases	Alias
Intellectual Developmental Disorder, Autosomal Dominant 22	MRD22 Mental Retardation, Autosomal Dominant 22 Autosomal Dominant Non-Syndromic Intellectual Disability 22 Distal Monosomy 1q Autosomal Dominant Intellectual Developmental Disorder 22 Autosomal Dominant Mental Retardation 22 Distal Deletion 1q Monosomy 1qter Telomeric Deletion 1q Mental Retardation, Autosomal Dominant, Type 22
Torch Syndrome

Diseases	Alias
Waldenstroem'S Macroglobulinemia	Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma Waldenstroem'S Macroglobulinemia Waldenstroem'S Macroglobulinemia Macroglobulinemia Of Waldenstrom Lymphoplasmacytic Lymphoma With Igm Gammopathy Lymphoplasmacytic Lymphoma

Related Agents

Pre-clinical Phase

Pre-clinical Phase

Bioactive Molecules (4)

Cat. No.	Product Name	Effect	Purity	Rare Diseases
HY-111458	GSK2643943A	Inhibitor	98.79%	Unkown
HY-RS15531	USP20 Human Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS16871	Usp20 Mouse Pre-designed siRNA Set A	Pre-designed Set	/	Unkown
HY-RS23312	Usp20 Rat Pre-designed siRNA Set A	Pre-designed Set	/	Unkown

Orthologs Information

Species	Symbol	Source	ID
Canis familiaris	USP20	VGNC	VGNC:48182
Rattus norvegicus	USP20	RGD	RGD:1305621
Felis catus	USP20	VGNC	VGNC:66872
Macaca mulatta	USP20	VGNC	VGNC:78740
Bos taurus	USP20	VGNC	VGNC:36715
Mus musculus	USP20	MGD	MGI:1921520
Others	USP20	NCBI