Hispolon

Name: Hispolon
Brand: MedChemExpress
SKU: HY-150521
Rating: 4.5 (1 reviews)

Cat. No.: HY-150521 Purity: 99.42%: Data Sheet
COA

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Hispolon, a polyphenol, can be isolated from Phellinus linteus. Hispolon possesses anticancer, antidiabetic, antioxidant, antiviral, hepatoprotective, anti-diabetic, and anti-inflammatory activities.

For research use only. We do not sell to patients.

Hispolon Chemical Structure

CAS No. : 173933-40-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hispolon:

Hispolon (Standard) Get quote

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A10	IC₅₀	37.05 μg/mL Compound: 1	Antiproliferative activity against rat A10 cells after 24 hrs by MTT assay Antiproliferative activity against rat A10 cells after 24 hrs by MTT assay	[PMID: 22967007]
A549	IC₅₀	0.183 μM Compound: 8	Cytotoxicity against human A549 cells after 96 hrs by MTT assay Cytotoxicity against human A549 cells after 96 hrs by MTT assay	[PMID: 15165144]
BGC-823	IC₅₀	0.205 μM Compound: 8	Cytotoxicity against human BGC823 cells after 96 hrs by MTT assay Cytotoxicity against human BGC823 cells after 96 hrs by MTT assay	[PMID: 15165144]
Bel-7402	IC₅₀	0.038 μM Compound: 8	Cytotoxicity against human Bel7402 cells after 96 hrs by MTT assay Cytotoxicity against human Bel7402 cells after 96 hrs by MTT assay	[PMID: 15165144]
DU-145	IC₅₀	28.6 μM Compound: VA-1	Cytotoxicity against human DU145 cells after 72 hrs by MTT assay Cytotoxicity against human DU145 cells after 72 hrs by MTT assay	[PMID: 25842364]
HCT-116	IC₅₀	5.2 μM Compound: VA-1	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 25842364]
HCT-8	IC₅₀	0.199 μM Compound: 8	Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay Cytotoxicity against human HCT8 cells after 96 hrs by MTT assay	[PMID: 15165144]
HEK293	IC₅₀	7.3 μM Compound: VA-1	Cytotoxicity against HEK293 cells expressing pcDNA3.1 after 72 hrs by MTT assay Cytotoxicity against HEK293 cells expressing pcDNA3.1 after 72 hrs by MTT assay	[PMID: 25842364]
HEK293	IC₅₀	9.6 μM Compound: VA-1	Cytotoxicity against HEK293 cells expressing ABCB1 after 72 hrs by MTT assay Cytotoxicity against HEK293 cells expressing ABCB1 after 72 hrs by MTT assay	[PMID: 25842364]
KETR3	IC₅₀	0.206 μM Compound: 8	Cytotoxicity against human Ketr3 cells after 96 hrs by MTT assay Cytotoxicity against human Ketr3 cells after 96 hrs by MTT assay	[PMID: 15165144]
MCF7	IC₅₀	0.025 μM Compound: 8	Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay Cytotoxicity against human MCF7 cells after 96 hrs by MTT assay	[PMID: 15165144]
MCF7	IC₅₀	7.9 μM Compound: VA-1	Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay Cytotoxicity against human MCF7 cells after 72 hrs by MTT assay	[PMID: 25842364]
MDA-MB-231	IC₅₀	32.2 μM Compound: VA-1	Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 72 hrs by MTT assay	[PMID: 25842364]
MDCK	IC₅₀	36.2 μM Compound: VA-1	Cytotoxicity against MDCK cells after 72 hrs by MTT assay Cytotoxicity against MDCK cells after 72 hrs by MTT assay	[PMID: 25842364]
NIH3T3	IC₅₀	51.2 μM Compound: VA-1	Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay Cytotoxicity against mouse NIH/3T3 cells after 72 hrs by MTT assay	[PMID: 25842364]
PC-3	IC₅₀	12.9 μM Compound: VA-1	Cytotoxicity against human PC3 cells after 72 hrs by MTT assay Cytotoxicity against human PC3 cells after 72 hrs by MTT assay	[PMID: 25842364]
S1	IC₅₀	8.4 μM Compound: VA-1	Cytotoxicity against human S1 cells after 72 hrs by MTT assay Cytotoxicity against human S1 cells after 72 hrs by MTT assay	[PMID: 25842364]

In Vitro

Hispolon (25 and 50 μM, 24-72 h) inhibits cell viability of U87MG cells^[2].
Hispolon (25 and 50 μM, 24, 48 h) induces G2/M cell cycle arrest and apoptosis in U87MG cells^[2].
Hispolon (25 and 50 μM, 2-8 h) decreases the expression of G1–S transition-related protein cyclin D4 but increases the expression of CDK inhibitor p21^[2].
Hispolon (25 and 50 μM, 24 h) inhibits the migration of U87MG cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:	U87MG cells
Concentration:	25 and 50 μM
Incubation Time:	24, 48, 72 h
Result:	Inhibited cell viability in a dose and time dependent way.

Western Blot Analysis^[2]

Cell Line:	U87MG cells
Concentration:	25 and 50 μM
Incubation Time:	2, 4, 8 h
Result:	Decreased cyclin D4 level, and increased p21 level.

In Vivo

Hispolon (2.5-10 mg/kg, i.p.) attenuates LPS-induced acute lung injury in mice^[3].
Hispolon (5 and 10 mg/kg, s.c.) reduces tumor growth in DBTRG xenograft mice^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS-induced acute lung injury mice^[3]
Dosage:	2.5, 5 and 10 mg/kg
Administration:	Intraperitoneal injection (i.p.)
Result:	Alleviated the pathological effects in the LPS-challenged mouse. Reduced the W/D ratio in the lung and MPO activity. Decreased pro-Inflammatory cytokine production.

Animal Model:	DBTRG xenograft mice^[4]
Dosage:	5 and 10 mg/kg
Administration:	Subcutaneous injection (s.c.)
Result:	Reduced tumor volume (RTV). Inhibited GBM cell proliferation in vivo upon HE and ki-67 staining.

Molecular Weight

220.22

Formula

C₁₂H₁₂O₄

CAS No.

173933-40-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

CC(/C=C(O)/C=C/C1=CC(O)=C(C=C1)O)=O

Structure Classification

Initial Source

Microorganisms

Phellinus linteus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (454.09 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.5409 mL	22.7046 mL	45.4091 mL
5 mM	0.9082 mL	4.5409 mL	9.0818 mL
10 mM	0.4541 mL	2.2705 mL	4.5409 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (11.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.42%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (245 KB) HNMR (252 KB) LCMS (98 KB)

Handling Instructions (2659 KB)

References

[1]. Sarfraz A, et al. Hispolon: A natural polyphenol and emerging cancer killer by multiple cellular signaling pathways. Environ Res. 2020 Nov;190:110017.

[2]. Arcella A, et al. Effects of hispolon on glioblastoma cell growth. Environ Toxicol. 2017 Sep;32(9):2113-2123. [Content Brief]

[3]. Huang CY, et al. Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy. Nutrients. 2020 Jun 10;12(6):1742. [Content Brief]

[4]. Liao KF, et al. Hispolon Induces Apoptosis, Suppresses Migration and Invasion of Glioblastoma Cells and Inhibits GBM Xenograft Tumor Growth In Vivo. Molecules. 2021 Jul 26;26(15):4497. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.5409 mL	22.7046 mL	45.4091 mL	113.5228 mL
	5 mM	0.9082 mL	4.5409 mL	9.0818 mL	22.7046 mL
	10 mM	0.4541 mL	2.2705 mL	4.5409 mL	11.3523 mL
	15 mM	0.3027 mL	1.5136 mL	3.0273 mL	7.5682 mL
	20 mM	0.2270 mL	1.1352 mL	2.2705 mL	5.6761 mL
	25 mM	0.1816 mL	0.9082 mL	1.8164 mL	4.5409 mL
	30 mM	0.1514 mL	0.7568 mL	1.5136 mL	3.7841 mL
	40 mM	0.1135 mL	0.5676 mL	1.1352 mL	2.8381 mL
	50 mM	0.0908 mL	0.4541 mL	0.9082 mL	2.2705 mL
	60 mM	0.0757 mL	0.3784 mL	0.7568 mL	1.8920 mL
	80 mM	0.0568 mL	0.2838 mL	0.5676 mL	1.4190 mL
	100 mM	0.0454 mL	0.2270 mL	0.4541 mL	1.1352 mL

Hispolon Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Hispolon173933-40-9AntibioticAnticancerantioxidantanti-inflammatoryU87MGLPSlung injuryInhibitorinhibitorinhibit

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Hispolon

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Other Forms of Hispolon:

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References

Customer Review

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Complete Stock Solution Preparation Table

Hispolon Related Classifications

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