Cat. No.: HY-100586

Ibuprofen L-lysine

(Synonyms: (±)-Ibuprofen L-lysine)

23 Cited Publications

Customer Review

Based on 23 publication(s) in Google Scholar

Ibuprofen ((±)-Ibuprofen) L-lysine is a potent orally active, selective COX-1 inhibitor with an IC₅₀ value of 13 μM. Ibuprofen L-lysine inhibits cell proliferation, angiogenesis, and induces cell apoptosis. Ibuprofen L-lysine is a nonsteroidal anti-inflammatory agent and a nitric oxide (NO) donor. Ibuprofen L-lysine can be used in the research of pain, swelling, inflammation, infection, immunology, cancers.

For research use only. We do not sell to patients.

Purity: 99.91%
CAS No.: 57469-77-9
Formula: C₁₉H₃₂N₂O₄
Molecular Weight:352.47

Storage:

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Ibuprofen L-lysine Chemical Structure

CAS No.: 57469-77-9

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (23)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

IC50: 13 μM (COX-1), 370 μM (COX-2)

All COX Isoforms :

All Parasite Isoforms :

In Vitro

Ibuprofen (24 h) L-lysine inhibits COX-1 and COX-2 activity with IC₅₀ values of 13 μM and 370 μM^[1].
Ibuprofen (500 μM, 48 h) L-lysine inhibits cell proliferation and angiogenesis, and induces apoptosis in AGS cells (Adenocarcinoma gastric cell line)^[2].
Ibuprofen (500 μM, 48 h) L-lysine downregulates transcription of Akt, VEGF-A, PCNA, Bcl2, OCT3/4 and CD44 genes, but upregulates RNA levels of wild type P53 and Bax genes in AGS cell^[2].
Ibuprofen (500 μM, 24 h) L-lysine restores microtubule reformation, microtubule-dependent intracellular cholesterol transport, and induces extension of microtubules to the cell periphery in both cystic fibrosis (CF) cell models and primary CF nasal epithelial cells^[3].
Ibuprofen (500 μM, 24 h) L-lysine enhances UV-induced cell death in MCF-7 cells and MDA-MB-231 cells by a photosensitization process^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

AGS cells
Concentration:

100-1000 μM
Incubation Time:

24 h, 48 h
Result:

Inhibited AGS cell viability with IC₅₀ values of 630 μM (trypan blue staining, 24 h), 456 μM (neutral red assay, 24 h), 549 μM (trypan blue staining, 48 h) and 408 μM (neutral red assay, 48 h).

In Vivo

Ibuprofen (300 mg/kg; p.o.; daily, for 14 days) L-lysine reduces overall tumor growth and enhances anti-tumor immune characteristics without adverse autoimmune reactions in a model of postpartum breast cancer^[5].
Ibuprofen (60 mg/kg; i.h.; every second day for 15 days) L-lysine reduces the risk of neuropathy in a rat model of chronic Oxaliplatin induced peripheral neuropathy^[6].
Ibuprofen (20 mg/kg; p.o.; every 12 hours, 5 doses total) L-lysine decreases muscle growth (average muscle fiber cross-sectional area) without affecting regulation of supraspinatus tendon adaptions to exercise^[7].
Ibuprofen (35 mg/kg; p.o.; twice daily) L-lysine attenuates the Inflammatory response to pseudomonas aeruginosa in a rat model of chronic pulmonary infection^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Syngeneic (D2A1) orthotopic Balb/c mouse model of PPBC (postpartum)^[5]
Dosage:

300 mg/kg, daily for 14 days
Administration:

Fed in animal feedings (added to pulverized standard chow and mixed dry, then mixed with water, made into chow pellets and dried thoroughly)
Result:

Suppresed tumor growth, reduced presence of immature monocytes and increased numbers of T cells.
Enhanced Th1 associated cytokines as well as promoted tumor border accumulation of T cells.

Animal Model:

Oxaliplatin‑induced peripheral neuropathy^[6]
Dosage:

60 mg/kg, every second day for 15 days
Administration:

Subcutaneous injection
Result:

Lowered sensory nerve conduction velocity (SNCV).

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 57469-77-9
Appearance Solid
Molecular Weight 352.47
Formula C₁₉H₃₂N₂O₄
Color White to off-white
SMILES

CC(C(O)=O)C1=CC=C(CC(C)C)C=C1.OC([C@@H](N)CCCCN)=O
Synonyms

(±)-Ibuprofen L-lysine
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Publications (23)

All In Vivo Efficacy StudyCell Proliferation/Viability AssayCell Migration/Invasion AssayFlow CytometryBio/Physico-chemical AssayWB

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Journal Impact Factor

Biomaterials

GSH-activable and cytolytic iPep-coupled immune nanoagonist for cancer synergetic therapy. [Abstract]

2025 Nov:322:123402. PMID: 40373515
Phytomedicine

Prim-O-glucosycimifugin attenuates liver injury in septic mice by inhibiting NLRP3 inflammasome/caspase-1 signaling cascades in macrophages. [Abstract]

2022 Nov:106:154427. PMID: 36088791
J Hazard Mater Lett

2025 Nov.
Int J Biol Macromol

Molecular interaction of abemaciclib with human serum albumin: Insights from spectroscopy, microscopy, and computational approaches. [Abstract]

2025 Dec 9;337(Pt 2):149613. PMID: 41380878
Plant Physiol

The MdSP1-MdANK immune module activated by effector SyCD1 enhances Malus domestica resistance to Valsa mali. [Abstract]

2026 Mar 2;200(3):kiag108. PMID: 41757702
Cell Rep

LAMP2A-mediated neuronal hyperexcitability by enhancing NKAβ1 degradation underlies depression-induced allodynia. [Abstract]

2025 Apr 2;44(4):115489. PMID: 40178973
Cell Rep

A Central Amygdala Input to the Parafascicular Nucleus Controls Comorbid Pain in Depression. [Abstract]

2019 Dec 17;29(12):3847-3858.e5. PMID: 31851918

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2019 Dec 17;29(12):3847-3858.e5. [Abstract]

Effects of Ibuprofen (10 mg/kg, i.p.) or indomethacin (10 mg/kg, i.p.) on pain thresholds at day 3 after CFA treatment.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Cell Rep. 2019 Dec 17;29(12):3847-3858.e5. [Abstract]

Effects of Ibuprofen (10 mg/kg, i.p.), Indomethacin, Gabapentin, or Lidocaine on pain thresholds of CRS 3W mice.
Biochem Pharmacol

The 'ABC' of split-nanoluciferase HIF heterodimerization bioassays: Applications, Benefits & Considerations. [Abstract]

2024 Nov:229:116478. PMID: 39128589
Inflammopharmacology

Ibuprofen inhibits anaplastic thyroid cells in vivo and in vitro by triggering NLRP3-ASC-GSDMD-dependent pyroptosis. [Abstract]

2024 Feb;32(1):733-745. PMID: 37999895

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

The inhibitory effect of Ibuprofen (0-3 mM, 48 h) on the proliferation of C643 and OCUT-2C cells was detected by CCK8 assay.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

Transwell assay, cells were treated with various concentrations of Ibuprofen (C643 at 0, 0.5, 1, and 1.5 mM; OCUT-2C at 0, 1, 2, and 3 mM) for 48 h view cell invasion abilities.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

Wound healing assay, cells were treated with different concentrations of Ibuprofen (C643 at 0, 0.5, 1, and 1.5 mM; OCUT-2C at 0, 1, 2, and 3 mM) for 48 h view cell migration abilities.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

Flow cytometry analysis of ATC cells treated with different Ibuprofen (0-3 mM) concentrations for 48 h stained with Annexin V-FITC and PI.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

The effect of Ibuprofen (0-1.5 mM) on LDH activity in C643 and OCUT-2C cells.

Ibuprofen L-lysine purchased from MedChemExpress. Usage Cited in: Inflammopharmacology. 2024 Feb;32(1):733-745. [Abstract]

Ibuprofen-induced expression of GSDMD protein in C643 and OCUT-2C cells.
Cells

Prostaglandin 2α Promotes Autophagy and Mitochondrial Energy Production in Fish Hepatocytes. [Abstract]

2022 Jun 9;11(12):1870. PMID: 35740999
EMBO Rep

CDK inhibitor Palbociclib targets STING to alleviate autoinflammation. [Abstract]

2022 Jun 7;23(6):e53932. PMID: 35403787
Cell Rep Methods

RECOVER identifies synergistic drug combinations in vitro through sequential model optimization. [Abstract]

2023 Oct 23;3(10):100599. PMID: 37797618
Int Immunopharmacol

Oxyphenbutazone suppresses non-canonical inflammasome activation and protects against LPS-induced sepsis. [Abstract]

2026 Aug 15:183:116873. PMID: 42150290
Int J Mol Sci

Singapore Grouper Iridovirus Disturbed Glycerophospholipids Homeostasis: Cytosolic Phospholipase A2 Was Essential for Virus Replication. [Abstract]

2021 Nov 22;22(22):12597. PMID: 34830477
Bioresour Technol Rep

2023 Nov 10, 101696.
Bioresour Technol Rep

2023 Dec, 24, 101619.
Sci Rep

Characterization of five novel vasopressin V2 receptor mutants causing nephrogenic diabetes insipidus reveals a role of tolvaptan for M272R-V2R mutation. [Abstract]

2020 Oct 2;10(1):16383. PMID: 33009446
Neuropharmacology

Malfunction of astrocyte and cholinergic input is involved in postoperative impairment of hippocampal synaptic plasticity and cognitive function. [Abstract]

2022 Oct 1:217:109191. PMID: 35835213
ACS Chem Neurosci

Identification of a Nonelectrophilic and Selective TRPA1 Agonist for Alleviation of Inflammatory Pain through Channel Desensitization. [Abstract]

2025 Sep 3;16(17):3257-3266. PMID: 40846466
J Neurosci

Acute Ongoing Nociception Delays Recovery of Consciousness from Sevoflurane Anesthesia via a Midbrain Circuit. [Abstract]

2024 Aug 21;44(34):e0740242024. PMID: 39019613
Reprod Toxicol

Zebrafish embryos as a teratogenicity screening tool to reduce potential birth defects. [Abstract]

2025 May:134:108895. PMID: 40097051
J Appl Toxicol

Differential impacts of nonsteroidal anti-inflammatory drugs on lifespan and healthspan in aged Caenorhabditis elegans. [Abstract]

2024 Oct;44(10):1528-1539. PMID: 38840409
Chemosphere

Mass-balance-model-based evaluation of sewage treatment plant contribution to residual pharmaceuticals in environmental waters. [Abstract]

2019 Jun:225:378-387. PMID: 30884299

Solvent & Solubility

In Vitro:

H₂O : 100 mg/mL (283.71 mM; Need ultrasonic)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8371 mL

14.1856 mL

28.3712 mL

5 mM

0.5674 mL

2.8371 mL

5.6742 mL

10 mM

0.2837 mL

1.4186 mL

2.8371 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Noreen Y, et al. Development of a radiochemical cyclooxygenase-1 and -2 in vitro assay for identification of natural products as inhibitors of prostaglandin biosynthesis. J Nat Prod. 1998 Jan;61(1):2-7. [Content Brief]

[2]. Hassan Akrami, et al. Inhibitory effect of ibuprofen on tumor survival and angiogenesis in gastric cancer cell. Tumour Biol. 2015 May;36(5):3237-43. [Content Brief]

[3]. Sharon M Rymut, et al. Ibuprofen regulation of microtubule dynamics in cystic fibrosis epithelial cells. Am J Physiol Lung Cell Mol Physiol. 2016 Aug 1;311(2):L317-27. [Content Brief]

[4]. Emmanuelle Bignon, et al. Ibuprofen and ketoprofen potentiate UVA-induced cell death by a photosensitization process. Sci Rep. 2017 Aug 21;7(1):8885. [Content Brief]

[5]. Nathan D Pennock, et al. Ibuprofen supports macrophage differentiation, T cell recruitment, and tumor suppression in a model of postpartum breast cancer. J Immunother Cancer. 2018 Oct 1;6(1):98. [Content Brief]

[6]. Thomas Krøigård, et al. Protective effect of ibuprofen in a rat model of chronic oxaliplatin-induced peripheral neuropathy. Exp Brain Res. 2019 Oct;237(10):2645-2651. [Content Brief]

[7]. Sarah Ilkhanipour Rooney, et al. Ibuprofen Differentially Affects Supraspinatus Muscle and Tendon Adaptations to Exercise in a Rat Model. Am J Sports Med. 2016 Sep;44(9):2237-45. [Content Brief]

[8]. M W Konstan, et al. Ibuprofen attenuates the inflammatory response to Pseudomonas aeruginosa in a rat model of chronic pulmonary infection. Implications for antiinflammatory therapy in cystic fibrosis. Am Rev Respir Dis. 1990 Jan;141(1):186-92. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
H₂O	1 mM	2.8371 mL	14.1856 mL	28.3712 mL	70.9280 mL
	5 mM	0.5674 mL	2.8371 mL	5.6742 mL	14.1856 mL
	10 mM	0.2837 mL	1.4186 mL	2.8371 mL	7.0928 mL
	15 mM	0.1891 mL	0.9457 mL	1.8914 mL	4.7285 mL
	20 mM	0.1419 mL	0.7093 mL	1.4186 mL	3.5464 mL
	25 mM	0.1135 mL	0.5674 mL	1.1348 mL	2.8371 mL
	30 mM	0.0946 mL	0.4729 mL	0.9457 mL	2.3643 mL
	40 mM	0.0709 mL	0.3546 mL	0.7093 mL	1.7732 mL
	50 mM	0.0567 mL	0.2837 mL	0.5674 mL	1.4186 mL
	60 mM	0.0473 mL	0.2364 mL	0.4729 mL	1.1821 mL
	80 mM	0.0355 mL	0.1773 mL	0.3546 mL	0.8866 mL
	100 mM	0.0284 mL	0.1419 mL	0.2837 mL	0.7093 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Ibuprofen L-lysine

23 Cited Publications

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Ibuprofen L-lysine

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Publications Citing Use of MedChemExpress (MCE) Ibuprofen L-lysine

All COX Isoforms

All Parasite Isoforms

Biological Activity

Chemical Information

Publications (23)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Ibuprofen L-lysine Related Classifications

HY-100586 Related Classifications