Cat. No.: HY-W013075

Rutin trihydrate

(Synonyms: Rutoside trihydrate; Quercetin 3-O-rutinoside trihydrate)

34 Cited Publications

Customer Review

Based on 34 publication(s) in Google Scholar

Rutin (Rutoside) trihydrate is a multifunctional natural flavonoid glycoside. Rutin trihydrate has been demonstrating excellent antioxidant, anti-inflammatory, anti-diabetic, and anti-carcinogenic properties. Cardioprotective and neuroprotective activities .

For research use only. We do not sell to patients.

Purity: 96.15%
CAS No.: 250249-75-3
Formula: C₂₇H₃₆O₁₉
Molecular Weight:664.56

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Rutin trihydrate Chemical Structure

CAS No.: 250249-75-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (34)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Endogenous Metabolite Isoforms :

In Vitro

Rutin (Rutoside) trihydrate (0.5- 160 μM/ml; PC12 cells) decreases the acrylamide (ACR)-induced (5.46 mM) cytotoxicity significantly. ACR (5.46 mM) decreases cell viability in PC12 cells and pretreatment with rutin (0.5, 1, 1.5, 2.5, 5, 10, 20 and 40 μM/ml) increases the cell viability^[2].
Due to the ability of Rutin and/or its metabolites to cross the blood brain barrier, it has also been shown to modify the cognitive and various behavioral symptoms of neurodegenerative diseases^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Pretreatment of Rutin (100 and 200 mg/kg/day; i.p.) for long-term caused behavioural and neurochemical changes in aged WAG male rats^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 250249-75-3
Appearance Solid
Molecular Weight 664.56
Formula C₂₇H₃₆O₁₉
Color Light yellow to green yellow
SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO[C@H]3[C@@H]([C@@H]([C@H]([C@H](C)O3)O)O)O)O2)O)O)O)=C(C4=CC=C(O)C(O)=C4)OC5=CC(O)=CC(O)=C15.O.O.O
Synonyms

Rutoside trihydrate; Quercetin 3-O-rutinoside trihydrate
Structure Classification
- Flavonoids
- Flavones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (34)

All Histological Imaging/StainingWBELISAIFCell Proliferation/Viability Assay

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Journal Impact Factor

Signal Transduct Target Ther

Short-chain acyl-CoA dehydrogenase initiates mtDNA demethylation and leakage to fuel antitumor immunity in colorectal cancer. [Abstract]

2026 Mar 26;11(1):113. PMID: 41888107
Neuron

Metabolic reprogramming through histone lactylation in microglia and macrophages recruits CD8+ T lymphocytes and aggravates spinal cord injury. [Abstract]

2025 Jul 23;113(14):2280-2296.e8. PMID: 40328251
Adv Sci (Weinh)

SRSF5-Mediated Alternative Splicing of M Gene is Essential for Influenza A Virus Replication: A Host-Directed Target Against Influenza Virus. [Abstract]

2022 Dec;9(34):e2203088. PMID: 36257906
Cell Death Differ

Redox regulation of TRIM28 facilitates neuronal ferroptosis by promoting SUMOylation and inhibiting OPTN-selective autophagic degradation of ACSL4. [Abstract]

2025 Jun;32(6):1041-1057. PMID: 39875520
Phytomedicine

Combined transcriptomics and network pharmacology to elucidate the mechanisms of rutin in treating ischemic stroke rat. [Abstract]

2025 Sep 15:148:157267. PMID: 40975038

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Rutin (80 mg/kg, q.d., 3 days) reduces pathological damage. Scale bar = 50 μm, n = 6 per group.

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. (A-H) Western blot analysis of the protein expression level of NF-κB p65, NLRP3, Caspase-1, GSDMD in MCAO/R rats brain tissues and grayscale scanning analysis. Scale bar = 20 μm. n = 3 per group.

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. ELISA analysis of IL-1β, IL-18, IL-6, TNF-α levels in serum. Scale bar = 20 μm. n = 3 per group.

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Effect of rutin (80 mg/kg, q.d., 3 days) on NF-κB/NLRP3 signaling and cell localization in tMCAO rats. Representative immunofluorescence staining pictures of NLRP3 in brain tissues. Scale bar = 20 μm. n = 3 per group.

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Effect of rutin (40 μM, 24 h) on OGD/R-induced primary astrocyte during reperfusion. Representative immunofluorescence staining pictures of NLRP3, GSDMD in OGD/R-induced primary astrocyte. Scale bar = 20 μm, n = 3 per group.

Rutin trihydrate purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2025 Sep 15:148:157267. [Abstract]

Effect of rutin (5, 10, 20, and 40 μM, 24 h) on OGD/R-induced primary astrocyte. Cell viability was determined with CCK-8 kit.
Phytomedicine

Rutin alleviates EndMT by restoring autophagy through inhibiting HDAC1 via PI3K/AKT/mTOR pathway in diabetic kidney disease. [Abstract]

2023 Apr:112:154700. PMID: 36774842
Food Chem

Effects of sun drying combined with baking processes on the flavor quality of Chongqing Tuocha raw tea. [Abstract]

2025 Dec 30:497:146992. PMID: 41285060
Food Chem

Flavonoid-mediated metabolic underpinning quality variation in red bud-sport pear mutants. [Abstract]

2025 Oct 15:489:144992. PMID: 40466530
Food Chem

Discovery of novel ascorbic acid derivatives and other metabolites in fruit of Rosa roxburghii Tratt through untargeted metabolomics and feature-based molecular networking. [Abstract]

2023 Mar 30;405(Pt A):134807. PMID: 36370576
Food Chem

New insights into the influences of baking and storage on the nonvolatile compounds in oolong tea: A nontargeted and targeted metabolomics study. [Abstract]

2022 May 1:375:131872. PMID: 34953237
Food Res Int

Optimal Brassicaceae family microgreens from a phytochemical and sensory perspective. [Abstract]

2024 Oct:193:114812. PMID: 39160037
Antioxidants (Basel)

Bioactive Extracts and Constituents from Taraxacum mongolicum: Antioxidant, Anti-Inflammatory, Enzyme-Inhibitory, and Molecular Docking Studies. [Abstract]

2026 May 29;15(6):688. PMID: 42351994
Antioxidants (Basel)

Phenolic Profile and Bioactivity Changes of Lotus Seedpod and Litchi Pericarp Procyanidins: Effect of Probiotic Bacteria Biotransformation. [Abstract]

2023 Nov 7;12(11):1974. PMID: 38001827
Ind Crops Prod

2025 Dec 11;239:122458.
Food Funct

Blackthorn juice from central Italy exhibits selective anticancer activity by inducing apoptosis and autophagy in gastric carcinoma cells. [Abstract]

2025 Oct 27;16(21):8548-8563. PMID: 41085359
Eur J Pharmacol

Rutin protects the pancreas from inflammatory injury and oncogene-driven tumorigenesis by inhibiting acinar to ductal metaplasia. [Abstract]

2025 Jul 5:998:177536. PMID: 40120793
Int Immunopharmacol

Rutin ameliorates hepatic ischemia-reperfusion injury by targeting CD36 to suppress hepatocyte ferroptosis. [Abstract]

2026 Mar 1:172:116231. PMID: 41576568
ACS Omega

Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer. [Abstract]

2024 Feb 28;9(10):11870-11882. PMID: 38496939
Metabolites

2025 Dec 23;16(1):12.
Metabolites

Rutin Maintains the Thermogenic Phenotype of Beige Adipocytes and Concomitantly Suppresses Mitophagy Against Obesity in HFD Mice. [Abstract]

2025 Dec 23;16(1):12. PMID: 41590620
iScience

Rutin alleviates ventilator-induced lung injury by inhibiting NLRP3 inflammasome activation. [Abstract]

2023 Sep 9;26(10):107866. PMID: 37817937
Biology (Basel)

Antibacterial, Antibiofilm, and Wound Healing Activities of Rutin and Quercetin and Their Interaction with Gentamicin on Excision Wounds in Diabetic Mice. [Abstract]

2024 Aug 29;13(9):676. PMID: 39336103
Polym Bull (Berl)

2026 Mar 26;83(6):314.
Neurotoxicology

Rutin alleviates Pb-induced oxidative stress, inflammation and cell death via activating Nrf2/ARE system in SH-SY5Y cells. [Abstract]

2024 Sep:104:1-10. PMID: 39032614
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Hum Mutat

SELL Marks an Effector-Deficient CD8+ T Cell Subset That Promotes Intracerebral Hemorrhage and Responds to Rutin Therapy. [Abstract]

2026 Apr 2:2026:4033506. PMID: 41937927
Arch Endocrinol Metab

miR-590-5p mediates mitochondrial respiration, proliferation, and apoptosis in thyroid carcinoma cells via fibroblast growth factor receptor substrate 2. [Abstract]

2025 Aug 20;69(4):e240410. PMID: 40834280
Lett Drug Des Discov

2026 Feb 11.
Pharmacogn Mag

2023 Oct 12.
SSRN

2026 Jun 21.
SSRN

2025 Oct 6.
Eur Rev Med Pharmacol Sci

Mulberry leaf extracts and quercetin glycosides promote glycogen accumulation in astrocytes. [Abstract]

2025 Sep;29(9):425-433. PMID: 41059752
Regulatory Research and Medicine Evaluation

2025.
SSRN

2025 Mar 20.

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (75.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.5048 mL

7.5238 mL

15.0476 mL

5 mM

0.3010 mL

1.5048 mL

3.0095 mL

10 mM

0.1505 mL

0.7524 mL

1.5048 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.76 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Mel MMRD, et al. Formulation of microencapsulated rutin and evaluation of bioactivity and stability upon in vitro digestive and dialysis conditions. Int J Biol Macromol. 2020;159:316-323. [Content Brief]

[2]. Doostkam A, et al. Effect of Rutin on Diabetic Auditory Neuropathy in an Experimental Rat Model. Clin Exp Otorhinolaryngol. 2021;14(3):259-267. [Content Brief]

[3]. Habtemariam S. Rutin as a Natural Therapy for Alzheimer's Disease: Insights into its Mechanisms of Action. Curr Med Chem. 2016;23(9):860-873. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.5048 mL	7.5238 mL	15.0476 mL	37.6189 mL
	5 mM	0.3010 mL	1.5048 mL	3.0095 mL	7.5238 mL
	10 mM	0.1505 mL	0.7524 mL	1.5048 mL	3.7619 mL
	15 mM	0.1003 mL	0.5016 mL	1.0032 mL	2.5079 mL
	20 mM	0.0752 mL	0.3762 mL	0.7524 mL	1.8809 mL
	25 mM	0.0602 mL	0.3010 mL	0.6019 mL	1.5048 mL
	30 mM	0.0502 mL	0.2508 mL	0.5016 mL	1.2540 mL
	40 mM	0.0376 mL	0.1881 mL	0.3762 mL	0.9405 mL
	50 mM	0.0301 mL	0.1505 mL	0.3010 mL	0.7524 mL
	60 mM	0.0251 mL	0.1254 mL	0.2508 mL	0.6270 mL

Keywords

Rutin trihydrate

34 Cited Publications

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Rutin trihydrate

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Publications Citing Use of MedChemExpress (MCE) Rutin trihydrate

All Endogenous Metabolite Isoforms

Biological Activity

Chemical Information

Publications (34)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Rutin trihydrate Related Classifications

HY-W013075 Related Classifications