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  3. LLL12

LLL12 is a small molecule inhibitor of STAT3 that inhibits STAT3 phosphorylation. LLL12 enhanced the inhibitory effect of Cisplatin (HY-17394) and Paclitaxel (HY-B0015) on ovarian cancer cell formation, migration, and growth.

For research use only. We do not sell to patients.

LLL12 Chemical Structure

LLL12 Chemical Structure

CAS No. : 1260247-42-4

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1 mg USD 120 In-stock
5 mg USD 350 In-stock
10 mg USD 580 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

LLL12 is a small molecule inhibitor of STAT3 that inhibits STAT3 phosphorylation. LLL12 enhanced the inhibitory effect of Cisplatin (HY-17394) and Paclitaxel (HY-B0015) on ovarian cancer cell formation, migration, and growth[1].

IC50 & Target[1]

p-STAT3

 

In Vitro

LLL12 (0.01-1 μM; 72 h) inhibits cell viability of ovarian cancer cell lines with or without Cisplatin (0.5 μM, 2.5 μM) and Paclitaxel (0.25 μM, 0.5 μM).[1].
LLL12 (0.25-1.0 μM; 72 h) inhibit STAT3 phosphorylation (Tyr705) in ovarian cancer cell lines[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: A2780, SKOV3, CAOV-3 and OVCAR5 ovarian cancer cell lines
Concentration: 0.25, 0.5, and 1 μM for A2780 and OVCAR5; 1, 2.5, and 5 μM for SKOV3 and CAOV-3
Incubation Time: 72 hours
Result: Inhibited STAT3 phosphorylation at Tyr705.
In Vivo

LLL12 (5 mg/kg; i.p.; once daily for 13 d) shows strong growth inhibition activity in mouse osteosarcoma cells and tumor growth[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mouse xenografts with SJSA or OS-33 osteosarcoma cells[2]
Dosage: 5 mg/kg
Administration: Intraperitoneal injection; once daily for 13 days
Result: Resulted in a significant reduction in tumor volume and tumor mass in the OS-33 and SJSA xenografted mice.
Molecular Weight

303.29

Formula

C14H9NO5S

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

O=S(C(C=CC=C1C(C2=C3C=CC=C2O)=O)=C1C3=O)(N)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (329.72 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2972 mL 16.4859 mL 32.9717 mL
5 mM 0.6594 mL 3.2972 mL 6.5943 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  2% DMSO    40% PEG300    5% Tween-80    53% Saline

    Solubility: 0.5 mg/mL (1.65 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2972 mL 16.4859 mL 32.9717 mL 82.4294 mL
5 mM 0.6594 mL 3.2972 mL 6.5943 mL 16.4859 mL
10 mM 0.3297 mL 1.6486 mL 3.2972 mL 8.2429 mL
15 mM 0.2198 mL 1.0991 mL 2.1981 mL 5.4953 mL
20 mM 0.1649 mL 0.8243 mL 1.6486 mL 4.1215 mL
25 mM 0.1319 mL 0.6594 mL 1.3189 mL 3.2972 mL
30 mM 0.1099 mL 0.5495 mL 1.0991 mL 2.7476 mL
40 mM 0.0824 mL 0.4121 mL 0.8243 mL 2.0607 mL
50 mM 0.0659 mL 0.3297 mL 0.6594 mL 1.6486 mL
60 mM 0.0550 mL 0.2748 mL 0.5495 mL 1.3738 mL
80 mM 0.0412 mL 0.2061 mL 0.4121 mL 1.0304 mL
100 mM 0.0330 mL 0.1649 mL 0.3297 mL 0.8243 mL
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LLL12 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LLL12
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