1. JAK/STAT Signaling Stem Cell/Wnt Apoptosis
  2. STAT Apoptosis
  3. Stattic

Stattic is a potent STAT3 inhibitor and inhibits STAT3 phosphorylation (at Y705 and S727). Stattic inhibits the binding of a high affinity phosphopeptide for the SH2 domain of STAT3. Stattic ameliorates the renal dysfunction in Alport syndrome (AS) mice.

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Stattic Chemical Structure

Stattic Chemical Structure

CAS No. : 19983-44-9

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10 mM * 1 mL in DMSO
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10 mM * 1 mL in DMSO USD 66 In-stock
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Customer Review

Based on 140 publication(s) in Google Scholar

Top Publications Citing Use of Products

134 Publications Citing Use of MCE Stattic

Proliferation Assay
WB

    Stattic purchased from MedChemExpress. Usage Cited in: Mol Med Rep. 2023 Apr;27(4):81.  [Abstract]

    Stattic (5, 15, 30 µM; 4 h) markedly inhibits Y705 STAT3 phosphorylation in both senescent TC‑1 (Fig. C) and TRAMP‑C2 (Fig. D) cells in a concentration‑dependent manner.

    Stattic purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2022 Nov 11;248:114268.  [Abstract]

    Stattic (10 μM) effectively attenuates the SCCP-mediated increase in iNOS and GFAP expression in C6 cells.

    Stattic purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2019 Mar 30;18(1):64.  [Abstract]

    Western blot analysis of HCT116 cells alone, IL6-supplemented HCT116, TAMs-co-cultured HCT116 in the presence or absence of Stattic (15 μM).

    Stattic purchased from MedChemExpress. Usage Cited in: Cell Signal. 2019 Mar;55:65-72.  [Abstract]

    Western blotting and densitometric analysis of p-STAT3 in Lo2 cells after 24 h of treatment with the indicated concentrations of the STAT3 inhibitor stattic.

    Stattic purchased from MedChemExpress. Usage Cited in: IUBMB Life. 2018 Jan;70(1):81-91.  [Abstract]

    A549 cells are treated with TG101348 (3 nM), BMS-911543 (1.5 nM), and Stattic (2.5 lM) for 24 h, and the conditioned media are collected and applied for tube formation assay in HUVECs.

    Stattic purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2017 Nov;56(11):2434-2445.  [Abstract]

    Cells are treated with the STAT3 inhibitor Stattic. The inhibitory action of Stattic on STAT3 phosphorylation is confirmed by Western blot.

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    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Stattic is a potent STAT3 inhibitor and inhibits STAT3 phosphorylation (at Y705 and S727)[1]. Stattic inhibits the binding of a high affinity phosphopeptide for the SH2 domain of STAT3[2]. Stattic ameliorates the renal dysfunction in Alport syndrome (AS) mice[3].

    IC50 & Target[1][2]

    STAT3

     

    In Vitro

    Stattic (20 μM; 24 hours) inhibits STAT3 phosphorylation (Y705) and selectively inhibits P-STAT3 as demonstrated by the lack of inhibition of P-ERK1/2 in ALDH+ and D44+/CD24+ subpopulations of Panc-1 and HPAC pancreatic cancer cell lines[1].
    ? Stattic (2.5, 5, 10 μM; for 4 h) significantly reduces the nuclear level of pSTAT3 and survivin in PC3M-1E8 cells at 10 μM. Stattic (2.5-10 μM; for 24 h) inhibits IL-6-induced STAT3 activation in a dose-dependent manner[2].
    ? Stattic (2.5, 5, 10 μM; for 48 h) suppresses both the growth and induces apoptosis prostate cancer cells (PC3M-1E8 cells) with 10 μM. Stattic does not induce significant cell apoptosis with 2.5 μM, 5 μM[2].
    ? Stattic (2.5, 5, 10 μM; for 48 h) shows significant S phase accumulation[2].
    ? Stattic can not lead to significant morphological changes or apoptosis and has little STAT3 phosphorylation in A2780 cells and HUVECs[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Stattic (10 mg/kg; i.p.; three times per week for 10 week) ameliorates the renal dysfunction in Alport syndrome (AS) mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Age-matched wild-type (WT) C57BL/6 mice[3]
    Dosage: 10 mg/kg
    Administration: IP; three timesper week for 10 week
    Result: Increased levels of proteinuria, BUN and serum creatinine.
    Significantly suppressed the gene expression levels of renal injury markers (Lcn2, Kim-1), pro-inflammatory cytokines (Il-6, KC), pro-fibrotic genes (Tgf-β, Col1a1, α-Sma) and Mmp9.
    Molecular Weight

    211.19

    Formula

    C8H5NO4S

    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=[N+](C1=CC=C(C=CS2(=O)=O)C2=C1)[O-]

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (236.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.7351 mL 23.6754 mL 47.3507 mL
    5 mM 0.9470 mL 4.7351 mL 9.4701 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (11.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (11.84 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.60%

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.7351 mL 23.6754 mL 47.3507 mL 118.3768 mL
    5 mM 0.9470 mL 4.7351 mL 9.4701 mL 23.6754 mL
    10 mM 0.4735 mL 2.3675 mL 4.7351 mL 11.8377 mL
    15 mM 0.3157 mL 1.5784 mL 3.1567 mL 7.8918 mL
    20 mM 0.2368 mL 1.1838 mL 2.3675 mL 5.9188 mL
    25 mM 0.1894 mL 0.9470 mL 1.8940 mL 4.7351 mL
    30 mM 0.1578 mL 0.7892 mL 1.5784 mL 3.9459 mL
    40 mM 0.1184 mL 0.5919 mL 1.1838 mL 2.9594 mL
    50 mM 0.0947 mL 0.4735 mL 0.9470 mL 2.3675 mL
    60 mM 0.0789 mL 0.3946 mL 0.7892 mL 1.9729 mL
    80 mM 0.0592 mL 0.2959 mL 0.5919 mL 1.4797 mL
    100 mM 0.0474 mL 0.2368 mL 0.4735 mL 1.1838 mL
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    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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