STAT5-IN-1
Based on 32 publication(s) in Google Scholar
STAT5-IN-1 is a STAT5 inhibitor with an IC50 of 47 μM for STAT5β isoform.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 98.35%
- No. CAS: 285986-31-4
- Fòrmula: C16H11N3O3
- Peso molecular:293.28
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
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Publications Citing Use of MedChemExpress (MCE) STAT5-IN-1
More- Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]
- Cell Metab. 2026 Jun 23:S1550-4131(26)00225-1. [Abstract]
- Cell Metab. 2023 Oct 3;35(10):1688-1703.e10. [Abstract]
- Nat Immunol. 2026 Mar;27(3):463-475. [Abstract]
- Cell Mol Immunol. 2025 Jul;22(7):791-805. [Abstract]
- Cancer Res. 2024 Mar 15;84(6):919-934. [Abstract]
- Nat Commun. 2026 Jan 23;17(1):952. [Abstract]
- Nat Commun. 2022 Nov 4;13(1):6648. [Abstract]
- Nat Commun. 2018 Nov 19;9(1):4874. [Abstract]
- Biomaterials. 2017 Jun:130:14-27. [Abstract]
- Cancer Lett. 2024 Apr 10:587:216730. [Abstract]
- Cell Death Discov. 2022 Mar 30;8(1):142. [Abstract]
- Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2114134119. [Abstract]
- Cell Mol Gastroenterol Hepatol. 2024;17(2):219-235. [Abstract]
- Cell Rep. 2022 Aug 2;40(5):111158. [Abstract]
- Sci Signal. 2020 Oct 27;13(655):eaay9217. [Abstract]
- Front Immunol. 2018 Aug 13:9:1755. [Abstract]
- JCI Insight. 2023 Apr 24;8(8):e167490. [Abstract]
- Hum Reprod. 2020 Dec 1;35(12):2677-2691. [Abstract]
- J Agric Food Chem. 2021 May 12;69(18):5353-5362. [Abstract]
- Biochem Pharmacol. 2020 Jan:171:113712. [Abstract]
- J Neuroimmune Pharmacol. 2026 Jan 31;21(1):9. [Abstract]
- Cells. 2026 Mar 14;15(6):521. [Abstract]
- Mucosal Immunol. 2024 Oct;17(5):793-809. [Abstract]
- Fish Shellfish Immunol. 2023 Feb:133:108519. [Abstract]
- Microbiol Spectr. 2023 Feb 14;11(1):e0493222. [Abstract]
- J Immunol. 2022 Apr 1;208(7):1616-1631. [Abstract]
- J Leukoc Biol. 2021 Aug;110(2):293-300. [Abstract]
- Cell Immunol. 2021 Apr:362:104286. [Abstract]
- Res Sq. 2025 May 12.
- Chemosphere. 2023 Jan;311(Pt 1):137068. [Abstract]
- Biol Blood Marrow Transplant. 2019 Oct;25(10):1911-1919. [Abstract]
Customer Validation & Images
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Flow Cytometry
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WB
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RT-PCR
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Apoptosis Analysis
Actividad biológica
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STAT5β 47 μM (IC50) |
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Neutrophil | IC50 |
>1000 μM
Compound: 18
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Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst
Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst
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[PMID: 20576329] |
The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT5-IN-1 inhibits STAT5 by binding to the SH2 domain. The functions of the SH2 domains of STAT3, STAT1, and of the tyrosine kinase Lck are inhibited to a lesser extent (IC50>500 μM). STAT5-IN-1 block STAT5/STAT5 DNA binding in K562 nuclear extracts. Substitution of the hydrogen at C6 of the chromone ring by an ethyl group does not affect activity of STAT5-IN-1 against STAT5β, but leads to complete loss of selectivity against other STAT family members[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 285986-31-4
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Appearance Solid
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Peso molecular 293.28
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Fòrmula C16H11N3O3
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Color White to light yellow
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SMILES
O=C(C1=CC=CN=C1)N/N=C/C2=COC3=CC=CC=C3C2=O
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 1 year -20°C 6 months
Publications (32)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Macrophages promote pre-metastatic niche formation of breast cancer through aryl hydrocarbon receptor activity. [Abstract]2024 Dec 18;9(1):352. PMID: 39690159
STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]
Western blotting analysis of AHR expression in STAT5-IN-1 (1, 5, 10 μM)-treated peritoneal macrophages, with or without 4T1-CM treatment for 24 h.
STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352. [Abstract]
mRNA levels of Ahr in peritoneal macrophages treated with or without 4T1-CM, in the presence or absence of STAT5-IN-1 (1, 5, or 10 μM) for 24 h.
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Cell Metab
Methionine-supplemented longevity diet increases growth hormone, GLP-1, and FGF21; reduces frailty; and promotes healthspan. [Abstract]2026 Jun 23:S1550-4131(26)00225-1. PMID: 42335894 -
Cell Metab
Neutrophils resist ferroptosis and promote breast cancer metastasis through aconitate decarboxylase 1. [Abstract]2023 Oct 3;35(10):1688-1703.e10. PMID: 37793345 -
Nat Immunol
Cell cycle arrest enhances CD8+ T cell effector function by potentiating glucose metabolism and IL-2 signaling. [Abstract]2026 Mar;27(3):463-475. PMID: 41555086 -
Cell Mol Immunol
γδ+ T-cell-derived IL-17A stimulates airway epithelial/stromal cells to secrete G-CSF, promoting lung-specific pathogenic Siglec-F+ neutrophil development in PPE-induced emphysema. [Abstract]2025 Jul;22(7):791-805. PMID: 40461699 -
Cancer Res
2024 Mar 15;84(6):919-934. PMID: 38231476 -
Nat Commun
The E3 ligases Itch and WWP2 regulate autoimmune neuroinflammation by controlling TH2 to TH17 cell conversion via interleukin-4-STAT5 axis in mice. [Abstract]2026 Jan 23;17(1):952. PMID: 41577662
STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Jan 23;17(1):952. [Abstract]
Frequency of IL-4 and IL-17A production in CD4+ T cells from DKO and TKO mice cultured in vitro under TH0 to TH17 conditions with the treatment of STAT5-IN (1, 10, 50 μM).
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Nat Commun
2022 Nov 4;13(1):6648. PMID: 36333322 -
Nat Commun
Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function. [Abstract]2018 Nov 19;9(1):4874. PMID: 30451838 -
Biomaterials
Nrf-2-driven long noncoding RNA ODRUL contributes to modulating silver nanoparticle-induced effects on erythroid cells. [Abstract]2017 Jun:130:14-27. PMID: 28351005 -
Cancer Lett
STAT5 is essential for inducing the suppressive subset and attenuate cytotoxicity of Vδ2+ T cells in acute myeloid leukemia. [Abstract]2024 Apr 10:587:216730. PMID: 38360140 -
Cell Death Discov
Ablation of Cbl-b and c-Cbl in dendritic cells causes spontaneous liver cirrhosis via altering multiple properties of CD103+ cDC1s. [Abstract]2022 Mar 30;8(1):142. PMID: 35354799
STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Mar 30;8(1):142. [Abstract]
STAT5-IN-1 (25, 50 μM) was added to CD103+ cDC cultures at the indicated concentration for 9 days after which apoptosis levels were analyzed by flow cytometry.
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Proc Natl Acad Sci U S A
2022 Jan 18;119(3):e2114134119. PMID: 35027451 -
Cell Mol Gastroenterol Hepatol
Oncostatin M induces IFITM1 expression to inhibit hepatitis B virus replication via JAK-STAT signaling. [Abstract]2024;17(2):219-235. PMID: 37879404 -
Cell Rep
Blocking DCIR mitigates colitis and prevents colorectal tumors by enhancing the GM-CSF-STAT5 pathway. [Abstract]2022 Aug 2;40(5):111158. PMID: 35926458 -
Sci Signal
2020 Oct 27;13(655):eaay9217. PMID: 33109748 -
Front Immunol
IL-2 Inhibition of Th17 Generation Rather Than Induction of Treg Cells Is Impaired in Primary Sjögren's Syndrome Patients. [Abstract]2018 Aug 13:9:1755. PMID: 30150979 -
JCI Insight
Single-cell profiling reveals pathogenic role and differentiation trajectory of granzyme K+ CD8+T cells in primary Sjögren's syndrome. [Abstract]2023 Apr 24;8(8):e167490. PMID: 36881472 -
Hum Reprod
Decidua-derived granulocyte macrophage colony-stimulating factor induces polymorphonuclear myeloid-derived suppressor cells from circulating CD15+ neutrophils. [Abstract]2020 Dec 1;35(12):2677-2691. PMID: 33067638 -
J Agric Food Chem
miR-204-5p and miR-211 Synergistically Downregulate the αS1-Casein Content and Contribute to the Lower Allergy of Goat Milk. [Abstract]2021 May 12;69(18):5353-5362. PMID: 33939400 -
Biochem Pharmacol
2020 Jan:171:113712. PMID: 31726048 -
J Neuroimmune Pharmacol
Low-dose IL-2 Ameliorates Experimental Autoimmune Myasthenia Gravis in Rats by Restoring the CD4-positive Helper T-cell Balance Via the JAK/STAT5 Pathway. [Abstract]2026 Jan 31;21(1):9. PMID: 41619069 -
Cells
2026 Mar 14;15(6):521. PMID: 41892312 -
Mucosal Immunol
Extracellular vesicles of Bacteroides uniformis induce M1 macrophage polarization and aggravate gut inflammation during weaning. [Abstract]2024 Oct;17(5):793-809. PMID: 38777177 -
Fish Shellfish Immunol
Large yellow croaker (Lrimichthys crocea) IL-2 modulates humoral immunity via the conserved JAK-STAT5 signal pathway. [Abstract]2023 Feb:133:108519. PMID: 36608811 -
Microbiol Spectr
Galectin-9 Facilitates Epstein-Barr Virus Latent Infection and Lymphomagenesis in Human B Cells. [Abstract]2023 Feb 14;11(1):e0493222. PMID: 36622166 -
J Immunol
IL-2 Signaling Couples the MAPK and mTORC1 Axes to Promote T Cell Proliferation and Differentiation in Teleosts. [Abstract]2022 Apr 1;208(7):1616-1631. PMID: 35321881 -
J Leukoc Biol
STAT5 promotes chronic pancreatitis by enhancing GM-CSF-dependent neutrophil augmentation. [Abstract]2021 Aug;110(2):293-300. PMID: 34184320 -
Cell Immunol
Regulation of ROS in myeloid-derived suppressor cells through targeting fatty acid transport protein 2 enhanced anti-PD-L1 tumor immunotherapy. [Abstract]2021 Apr:362:104286. PMID: 33524739 -
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Chemosphere
Cadmium impairs the development of natural killer cells and bidirectionally modifies their capacity for cytotoxicity. [Abstract]2023 Jan;311(Pt 1):137068. PMID: 36330983 -
Biol Blood Marrow Transplant
IL-22 Accelerates Thymus Regeneration via Stat3/Mcl-1 and Decreases Chronic Graft-versus-Host Disease in Mice after Allotransplants. [Abstract]2019 Oct;25(10):1911-1919. PMID: 31195136
Solvente y solubilidad
DMSO : 20 mg/mL (68.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2 mg/mL (6.82 mM); Clear solution
This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4097 mL | 17.0486 mL | 34.0971 mL | 85.2428 mL |
| 5 mM | 0.6819 mL | 3.4097 mL | 6.8194 mL | 17.0486 mL | |
| 10 mM | 0.3410 mL | 1.7049 mL | 3.4097 mL | 8.5243 mL | |
| 15 mM | 0.2273 mL | 1.1366 mL | 2.2731 mL | 5.6829 mL | |
| 20 mM | 0.1705 mL | 0.8524 mL | 1.7049 mL | 4.2621 mL | |
| 25 mM | 0.1364 mL | 0.6819 mL | 1.3639 mL | 3.4097 mL | |
| 30 mM | 0.1137 mL | 0.5683 mL | 1.1366 mL | 2.8414 mL | |
| 40 mM | 0.0852 mL | 0.4262 mL | 0.8524 mL | 2.1311 mL | |
| 50 mM | 0.0682 mL | 0.3410 mL | 0.6819 mL | 1.7049 mL | |
| 60 mM | 0.0568 mL | 0.2841 mL | 0.5683 mL | 1.4207 mL |
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