2. JAK/STAT Signaling Stem Cell/Wnt
  3. STAT
  4. STAT5-IN-1

STAT5-IN-1 is a STAT5 inhibitor with an IC50 of 47 μM for STAT5β isoform.

For research use only. We do not sell to patients.

CAS No. : 285986-31-4

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 31 publication(s) in Google Scholar

Other Forms of STAT5-IN-1:

STAT5-IN-1 Related Antibodies

Top Publications Citing Use of Products

31 Publications Citing Use of MCE STAT5-IN-1

WB
Flow Cytometry
Apoptosis Analysis
RT-PCR

    STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Jan 23;17(1):952.  [Abstract]

    Frequency of IL-4 and IL-17A production in CD4+ T cells from DKO and TKO mice cultured in vitro under TH0 to TH17 conditions with the treatment of STAT5-IN (1, 10, 50 μM).

    STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352.  [Abstract]

    Western blotting analysis of AHR expression in STAT5-IN-1 (1, 5, 10 μM)-treated peritoneal macrophages, with or without 4T1-CM treatment for 24 h.

    STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2024 Dec 18;9(1):352.  [Abstract]

    mRNA levels of Ahr in peritoneal macrophages treated with or without 4T1-CM, in the presence or absence of STAT5-IN-1 (1, 5, or 10 μM) for 24 h.

    STAT5-IN-1 purchased from MedChemExpress. Usage Cited in: Cell Death Discov. 2022 Mar 30;8(1):142.  [Abstract]

    STAT5-IN-1 (25, 50 μM) was added to CD103+ cDC cultures at the indicated concentration for 9 days after which apoptosis levels were analyzed by flow cytometry.

    View All STAT Isoform Specific Products:

    View All Isoforms
    STAT3 STAT5 STAT6 STAT1

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    STAT5-IN-1 is a STAT5 inhibitor with an IC50 of 47 μM for STAT5β isoform.

    IC50 & Target

    STAT5β

    47 μM (IC50)

    Cellular Effect
    Cell Line Type Value Description References
    Neutrophil IC50
    > 1000 μM
    Compound: 18
    Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst
    Antiinflammatory activity in human neutrophils assessed as inhibition of respiratory burst
    		[PMID: 20576329]
    In Vitro

    The signal transducer and activator of transcription 5 (STAT5) is a member of the STAT family of proteins, implicated in cell growth and differentiation. STAT5-IN-1 inhibits STAT5 by binding to the SH2 domain. The functions of the SH2 domains of STAT3, STAT1, and of the tyrosine kinase Lck are inhibited to a lesser extent (IC50>500 μM). STAT5-IN-1 block STAT5/STAT5 DNA binding in K562 nuclear extracts. Substitution of the hydrogen at C6 of the chromone ring by an ethyl group does not affect activity of STAT5-IN-1 against STAT5β, but leads to complete loss of selectivity against other STAT family members[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    STAT5-IN-1 Related Antibodies
    Molecular Weight

    293.28

    Formula

    C16H11N3O3
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=C(C1=CC=CN=C1)N/N=C/C2=COC3=CC=CC=C3C2=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : 20 mg/mL (68.19 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.4097 mL 17.0486 mL 34.0971 mL
    5 mM 0.6819 mL 3.4097 mL 6.8194 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2 mg/mL (6.82 mM); Clear solution

      This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  50% PEG300    50% Saline

      Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic

    • Protocol 2

      Add each solvent one by one:  0.5% CMC-Na/saline water

      Solubility: 12.5 mg/mL (42.62 mM); Suspended solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 98.35%

    SDS (393 KB)

    COA (247 KB) HNMR (386 KB) LCMS (92 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.4097 mL 17.0486 mL 34.0971 mL 85.2428 mL
    5 mM 0.6819 mL 3.4097 mL 6.8194 mL 17.0486 mL
    10 mM 0.3410 mL 1.7049 mL 3.4097 mL 8.5243 mL
    15 mM 0.2273 mL 1.1366 mL 2.2731 mL 5.6829 mL
    20 mM 0.1705 mL 0.8524 mL 1.7049 mL 4.2621 mL
    25 mM 0.1364 mL 0.6819 mL 1.3639 mL 3.4097 mL
    30 mM 0.1137 mL 0.5683 mL 1.1366 mL 2.8414 mL
    40 mM 0.0852 mL 0.4262 mL 0.8524 mL 2.1311 mL
    50 mM 0.0682 mL 0.3410 mL 0.6819 mL 1.7049 mL
    60 mM 0.0568 mL 0.2841 mL 0.5683 mL 1.4207 mL
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    STAT5-IN-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    STAT5-IN-1285986-31-4STATInhibitorinhibitorinhibit

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