2. Cell Cycle/DNA Damage
  3. DNA/RNA Synthesis
  4. ML-60218

ML-60218 is a broad-spectrum RNA pol III inhibitor, with IC50s of 32 and 27 μM for Saccharomyces cerevisiae and human. ML-60218 disrupts already assembled viroplasms and to hamper the formation of new ones without the need for de novo transcription of cellular RNAs.

For research use only. We do not sell to patients.

CAS No. : 577784-91-9

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Based on 8 publication(s) in Google Scholar

ML-60218 Related Antibodies

Top Publications Citing Use of Products

    ML-60218 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Dec 18;85(24):4526-4544.e18.  [Abstract]

    Western blot showing the level of p300, CBP, H3K27ac and H4K20ac in 4T1 cells treated with ML-60218 (25 μM) for 48 h (left) and 96 h (right). The results showed that after 48 hours of treatment with ML-60218, the levels of p300, CBP, H3K27ac, and H4K20ac increased.

    ML-60218 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2025 Dec 18;85(24):4526-4544.e18.  [Abstract]

    qRT-PCR analysis showing the levels of RNA polymerase III-transcribed genes in Pol III-inhibitor, ML-60218-treated (25 µM; 48 or 96 h) 4T1 cells at different time point.

    ML-60218 purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2022 Sep 5;20(1):96.  [Abstract]

    CCk8 assay was meant to detect MCF-7 cell viability under Pol III inhibitor ML-60218 (7.5-60 μM； 24-48 h) +/- nutlin-3.

    ML-60218 purchased from MedChemExpress. Usage Cited in: PLoS Pathog. 2022 Jan 28;18(1):e1010270.  [Abstract]

    PK-15 cells or PAMs (1×106) were pre-treated with the indicated concentrations of the RNA Pol-III inhibitor ML-60218 (20-50 μM) for 6 hours before transfection with poly(dA:dT), ASFV-AT, ASFV-GC or poly(I:C) (1 μg/mL) for 4 hours. The mRNA levels of IFNB1 were analyzed by RT-qPCR. The results showed that ML-60218, a inhibitor of RNA Pol‑III, markedly inhibited transcription of the IFNB1 gene induced by transfected poly(dA:dT) and ASFV/DNA‑AT, but not by poly(I:C) or ASFV/DNA‑GC, in porcine PK‑15 cells and PAMs, respectively.

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    Description

    ML-60218 is a broad-spectrum RNA pol III inhibitor, with IC50s of 32 and 27 μM for Saccharomyces cerevisiae and human. ML-60218 disrupts already assembled viroplasms and to hamper the formation of new ones without the need for de novo transcription of cellular RNAs[1][2].

    In Vitro

    Combination of SAHA and ML-60218 produces enhanced suppression of proliferation in human pancreatic adenocarcinoma by impairing cell cycle progression and inducing apoptosis. ML-60218 reverses SAHA-stimulated tRNA expression in PANC-1 and BxPC-3 cells. ML-60218 enhances the ability of HDAC inhibitors to induce apoptosis and cell cycle arrest[2].
    In in vitro transcription assays with purified double-layered particles (DLPs), ML-60218 shows dose-dependent inhibitory activity, indicating the viral nature of its target. ML-60218 is found to interfere with the formation of higher-order structures of VP6, the protein forming the DLP outer layer, without compromising its ability to trimerize. Electron microscopy of ML-60218-treated DLPs shows dose-dependent structural damage. ML-60218-mediated (10 μM ) viroplasm disruption causes NSP5 dephosphorylation[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ML-60218 Related Antibodies
    Molecular Weight

    452.38

    Formula

    C19H15Cl2N3O2S2
    CAS No.
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    O=S(C1=CC=CC=C1Cl)(NC2=CC(C3=C(C)C4=CC(Cl)=CC=C4S3)=NN2C)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (221.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2105 mL 11.0527 mL 22.1053 mL
    5 mM 0.4421 mL 2.2105 mL 4.4211 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

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    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
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    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.69%

    SDS (251 KB)

    COA (250 KB) RP-HPLC (262 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2105 mL 11.0527 mL 22.1053 mL 55.2633 mL
    5 mM 0.4421 mL 2.2105 mL 4.4211 mL 11.0527 mL
    10 mM 0.2211 mL 1.1053 mL 2.2105 mL 5.5263 mL
    15 mM 0.1474 mL 0.7368 mL 1.4737 mL 3.6842 mL
    20 mM 0.1105 mL 0.5526 mL 1.1053 mL 2.7632 mL
    25 mM 0.0884 mL 0.4421 mL 0.8842 mL 2.2105 mL
    30 mM 0.0737 mL 0.3684 mL 0.7368 mL 1.8421 mL
    40 mM 0.0553 mL 0.2763 mL 0.5526 mL 1.3816 mL
    50 mM 0.0442 mL 0.2211 mL 0.4421 mL 1.1053 mL
    60 mM 0.0368 mL 0.1842 mL 0.3684 mL 0.9211 mL
    80 mM 0.0276 mL 0.1382 mL 0.2763 mL 0.6908 mL
    100 mM 0.0221 mL 0.1105 mL 0.2211 mL 0.5526 mL
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    ML-60218 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ML-60218577784-91-9ML60218ML 60218DNA/RNA Synthesisbroad-spectrumyeastviroplasmstranscriptionInhibitorinhibitorinhibit

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