1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis
  3. BMH-21

BMH-21 

Cat. No.: HY-12484 Purity: 98.61%
Handling Instructions

BMH-21 is a first-in-class DNA intercalator which inhibits RNA polymerase I (Pol I) transcription. BMH-21 possesses anticancer activity.

For research use only. We do not sell to patients.

BMH-21 Chemical Structure

BMH-21 Chemical Structure

CAS No. : 896705-16-1

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10 mg USD 65 In-stock
Estimated Time of Arrival: December 31
50 mg USD 200 In-stock
Estimated Time of Arrival: December 31
100 mg USD 350 In-stock
Estimated Time of Arrival: December 31
500 mg USD 1200 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Top Publications Citing Use of Products
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Description

BMH-21 is a first-in-class DNA intercalator which inhibits RNA polymerase I (Pol I) transcription. BMH-21 possesses anticancer activity[1][2].

IC50 & Target
In Vitro

BMH-21 binds ribosomal DNA and inhibits Pol I transcription[1].
BMH-21 inhibits RNA polymerase I independent of DNA damage response[1].
BMH-21 intercalates into double strand (ds) DNA and has binding preference towards GC-rich DNA sequences[1].
BMH-21 (1 μM; 3 hours) acts in a DNA damage independent manner to activate nucleolar stress and RPA194 degradation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: U2OS cells
Concentration: 0 μM, 0.1 μM , 1 μM, 10 μM
Incubation Time: 48 hours
Result: Decreased the viability of cells at concentrations that activated the DNA damage response.

Western Blot Analysis[1]

Cell Line: A375 cells
Concentration: 1 μM
Incubation Time: 3 hours
Result: Degraded RPA194 with blocked ATM and DNA-PKcs activity.
In Vivo

BMH-21 (50 mg/kg; i.p.; daily; for 6 days) inhibits HCT116 colon cancer tumor growth in vivo[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 6-week old athymic NCr nu/nu mice, with HCT116 colorectal carcinoma xenograft[2]
Dosage: 50 mg/kg
Administration: Intraperitoneal injection, daily, for 6 days
Result: Significantly inhibited HCT116 colon cancer tumor growth.
Molecular Weight

360.41

Formula

C₂₁H₂₀N₄O₂

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 1 mg/mL (2.77 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7746 mL 13.8731 mL 27.7462 mL
5 mM --- --- ---
10 mM --- --- ---
*Please refer to the solubility information to select the appropriate solvent.
References
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Product Name:
BMH-21
Cat. No.:
HY-12484
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