MTEP

Name: MTEP
Brand: MedChemExpress
SKU: HY-13206A
Rating: 5.0 (3 reviews)

Cat. No.: HY-13206A Purity: 99.91%: Data Sheet
COA

SDS
Handling Instructions
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MTEP is a potent, non-competitive and highly selective mGluR5 antagonist, with an IC₅₀ of 5 nM and a K_i of 16 nM. MTEP shows antidepressant and anxiolytic-like effects. MTEP can be used for Parkinson's disease research. MTEP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

For research use only. We do not sell to patients.

MTEP Chemical Structure

CAS No. : 329205-68-7

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	Get quote
100 mg	Get quote

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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of MTEP:

MTEP hydrochloride In-stock

3 Publications Citing Use of MCE MTEP

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View All mGluR Isoform Specific Products:

View All Isoforms

mGluR1 mGluR2 mGluR3 mGluR4 mGluR5 mGluR6 mGluR7 mGluR8 mGluR

Biological Activity
Purity & Documentation
References
Customer Review

Description

MTEP is a potent, non-competitive and highly selective mGluR5 antagonist, with an IC₅₀ of 5 nM and a K_i of 16 nM. MTEP shows antidepressant and anxiolytic-like effects. MTEP can be used for Parkinson's disease research^[1]^[2]^[3]^[4]. MTEP is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.

IC₅₀ & Target

mGluR5

5 nM (IC₅₀)

mGluR5

16 nM (Ki)

Cellular Effect

Cell Line	Type	Value	Description	References
CHO	IC₅₀	0.462 μM Compound: 4	Antagonist activity against mGluR5 expressed in CHO cells assessed as inhibition of agonist-induced phosphoinositide hydrolysis Antagonist activity against mGluR5 expressed in CHO cells assessed as inhibition of agonist-induced phosphoinositide hydrolysis	[PMID: 16678408]
CHO	IC₅₀	462 nM Compound: 22	Activity in agonist-induced phosphoinositide hydrolysis in CHO cells expressing mGluR5a Activity in agonist-induced phosphoinositide hydrolysis in CHO cells expressing mGluR5a	[PMID: 16451073]
HEK293	IC₅₀	12 nM Compound: MTEP	Negative allosteric modulation of human mGluR5 expressed in HEK293 cells assessed as calcium mobilization by FLIPR assay Negative allosteric modulation of human mGluR5 expressed in HEK293 cells assessed as calcium mobilization by FLIPR assay	[PMID: 23434029]
HEK293	IC₅₀	5 nM Compound: MTEP	Antagonist activity at rat mGluR5 expressed in human HEK-293 cells assessed as inhibition of glutamate-induced intracellular calcium mobilization Antagonist activity at rat mGluR5 expressed in human HEK-293 cells assessed as inhibition of glutamate-induced intracellular calcium mobilization	[PMID: 21546249]
HEK293-A	IC₅₀	110 nM Compound: 2, MTEP	Inverse agonist activity at rat mGluR5 expressed in HEK293A cells coexpressing Gqalpha assessed as inhibition of quisqualic-induced D-myo-inositol 1 production by ELISA Inverse agonist activity at rat mGluR5 expressed in HEK293A cells coexpressing Gqalpha assessed as inhibition of quisqualic-induced D-myo-inositol 1 production by ELISA	[PMID: 22924094]
HEK293-A	IC₅₀	13.6 nM Compound: 2	Antagonist activity at rat mGluR5 expressed in HEK293A cells assessed as glutamate-induced calcium flux preincubated for 140 sec before glutamate challenge by calcium fluorescence assay Antagonist activity at rat mGluR5 expressed in HEK293A cells assessed as glutamate-induced calcium flux preincubated for 140 sec before glutamate challenge by calcium fluorescence assay	[PMID: 19445453]

In Vitro

MTEP shows highly selective for mGluR5 over mGluR1, has no effect on other mGluR subtypes, and has fewer off-target effects than MPEP (HY-14609A)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

MTEP (0-5 mg/kg, i.p., once) inhibits the catalepsy induced by Haloperidol (HY-14538) (0.5 mg/kg/2 ml i.p.)^[2].
MTEP (0.3-3 mg/kg, intraperitoneal injection, once) induces antidepressant-like effects in male C57BL/6J mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar rats (215-315 g, 5-9/group)^[2]
Dosage:	1, 3 and 5 mg/kg
Administration:	IP, once, injected 60 min after Haloperidol (HY-14538) (0.5 mg/kg/2 ml i.p.)
Result:	Inhibited the catalepsy induced by Haloperidol (HY-14538).

Animal Model:	Male C57BL/6J mice (23-25 g)^[3]
Dosage:	0.3, 1 and 3 mg/kg
Administration:	IP, 1 h before the test
Result:	Significantly decreased the immobility time of mice in the tail suspension test (TST) by 24%, 41% and 48%, respectively. The efficacy of MTEP used at doses of 1 and 3 mg/kg was not significantly different from that of Imipramine (HY-B1490A) (20 mg/kg, ip), used as a positive standard.

Molecular Weight

200.26

Formula

C₁₁H₈N₂S

CAS No.

329205-68-7

Appearance

Solid

Color

White to off-white

SMILES

CC1=NC(C#CC2=CC=CN=C2)=CS1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (499.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.9935 mL	24.9675 mL	49.9351 mL
5 mM	0.9987 mL	4.9935 mL	9.9870 mL
10 mM	0.4994 mL	2.4968 mL	4.9935 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.91%

Select Batch:

Data Sheet (275 KB) SDS (393 KB)

COA (247 KB) HNMR (414 KB) RP-HPLC (254 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Lea PM 4th, et al. Metabotropic glutamate receptor subtype 5 antagonists MPEP and MTEP. CNS Drug Rev. 2006 Summer;12(2):149-66. [Content Brief]

[2]. Ossowska K, et al. MTEP, a new selective antagonist of the metabotropic glutamate receptor subtype 5 (mGluR5), produces antiparkinsonian-like effects in rats. Neuropharmacology. 2005 Sep;49(4):447-55. [Content Brief]

[3]. Pałucha A, et al. Potential antidepressant-like effect of MTEP, a potent and highly selective mGluR5 antagonist. Pharmacol Biochem Behav. 2005 Aug;81(4):901-6. [Content Brief]

[4]. Klodzinska A, et al. Anxiolytic-like effects of MTEP, a potent and selective mGlu5 receptor agonist does not involve GABA(A) signaling. Neuropharmacology. 2004 Sep;47(3):342-50. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.9935 mL	24.9675 mL	49.9351 mL	124.8377 mL
	5 mM	0.9987 mL	4.9935 mL	9.9870 mL	24.9675 mL
	10 mM	0.4994 mL	2.4968 mL	4.9935 mL	12.4838 mL
	15 mM	0.3329 mL	1.6645 mL	3.3290 mL	8.3225 mL
	20 mM	0.2497 mL	1.2484 mL	2.4968 mL	6.2419 mL
	25 mM	0.1997 mL	0.9987 mL	1.9974 mL	4.9935 mL
	30 mM	0.1665 mL	0.8323 mL	1.6645 mL	4.1613 mL
	40 mM	0.1248 mL	0.6242 mL	1.2484 mL	3.1209 mL
	50 mM	0.0999 mL	0.4994 mL	0.9987 mL	2.4968 mL
	60 mM	0.0832 mL	0.4161 mL	0.8323 mL	2.0806 mL
	80 mM	0.0624 mL	0.3121 mL	0.6242 mL	1.5605 mL
	100 mM	0.0499 mL	0.2497 mL	0.4994 mL	1.2484 mL

MTEP Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MTEP329205-68-7mGluRMetabotropic glutamate receptorsAntidepressantanxiolytic-likeParkinson's diseaseInhibitorinhibitorinhibit

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MTEP

Customer Review

Other Forms of MTEP:

MTEP Related Antibodies

3 Publications Citing Use of MCE MTEP

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All mGluR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

MTEP Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

MTEP Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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SAFETY DATA SHEET (SDS)

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