N-Nitrosodiethylamine (Synonyms: Diethylnitrosamine; DEN; DENA)

Name: N-Nitrosodiethylamine
Brand: MedChemExpress
SKU: HY-N7434
Rating: 5.0 (6 reviews)

Cat. No.: HY-N7434 Purity: 99.98%: Data Sheet
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N-Nitrosodiethylamine (Diethylnitrosamine) is a potent hepatocarcinogenic dialkylnitrosoamine. N-Nitrosodiethylamine is mainly present in tobacco smoke, water, cheddar cheese, cured, fried meals and many alcoholic beverages. N-Nitrosodiethylamine is responsible for the changes in the nuclear enzymes associated with DNA repair/replication. N-Nitrosodiethylamine results in various tumors in all animal species. The main target organs are the nasal cavity, trachea, lung, esophagus and liver.

For research use only. We do not sell to patients.

CAS No. : 55-18-5

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	Get quote
50 g	Get quote

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Based on 6 publication(s) in Google Scholar

Other Forms of N-Nitrosodiethylamine:

N-Nitrosodiethylamine-d₄ In-stock
N-Nitrosodiethylamine-d₁₀ In-stock
N-Nitrosodiethylamine (Standard) Get quote

6 Publications Citing Use of MCE N-Nitrosodiethylamine

In Vivo Efficacy Study

RT-PCR

IHC

: N-Nitrosodiethylamine purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2025 Aug 10:217978. [Abstract]; N-Nitrosodiethylamine (DEN; 200 mg/kg; ip). The protein and mRNA levels of LGR5 were tested in tumor (T) and para-tumor (N) of DEN-induced hepatoma via western blot.

: N-Nitrosodiethylamine purchased from MedChemExpress. Usage Cited in: Cancer Lett. 2025 Aug 10:217978. [Abstract]; N-Nitrosodiethylamine (DEN; 200 mg/kg; ip). The protein and mRNA levels of LGR5 were tested in tumor (T) and para-tumor (N) of DEN-induced hepatoma via RT-qPCR.

: N-Nitrosodiethylamine purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2023 Jul;50(1):142. [Abstract]; N-Nitrosodiethylamine (DEN; 40 mg/kg; ip). Histopathology of liver tissue from mice with DEN-induced primary liver cancer after LNT treatment. Histopathology of mouse liver tissues.

: N-Nitrosodiethylamine purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2023 Jul;50(1):142. [Abstract]; N-Nitrosodiethylamine (DEN; 40 mg/kg; ip). Histopathology of liver tissue from mice with DEN-induced primary liver cancer. H&E staining of liver tissue sections from mice.

: N-Nitrosodiethylamine purchased from MedChemExpress. Usage Cited in: Oncol Rep. 2023 Jul;50(1):142. [Abstract]; N-Nitrosodiethylamine (DEN; 40 mg/kg; ip). Inhibitory effect of LNT on DEN-induced primary liver cancer in mice. The expression levels of EGR1, PTEN and other proteins in the liver tissues of the normal group and the model group, as analyzed by WB.

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Description

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

N-Nitrosodiethylamine can be used to create hepatocellular carcinoma models. In beagle dogs, after intravenous administration, the plasma concentration of N-Nitrosodiethylamine shows a biphasic decline, with an average distribution half-life of 19 minutes, an average elimination half-life of 73 minutes, an average total body clearance of 43.3 mL/min/kg, a steady-state distribution volume of 1.9 L/kg, and an average residence time of 45 minutes. The average bioavailability of the lowest oral dose reaches 93%^[3].

Induction of Liver Cancer^[1]^[2]

Background

Cytochrome P450 enzyme activates nitrosamine metabolism as an active electrophilic agent, making it cytotoxic, mutagenic and carcinogenic^[4].

Specific Modeling Methods

Male Wistar strain albino rats • 150-180 g • 12 weeks old
Administration: 0.01% NDEA through drinking water for 15 weeks • or 200 mg/kg • i.p. • single dose

Note

(1)The liver tissues were examined histologically at 1-3 weeks after the administration of NDEA.
(2)The rats were sacrificed by decapitation following overnight fast.

Modeling Indicators

Decreased final body weight and increased relative liver weight.
Molecular changes: A decrease in the activities of SOD, CAT, GPx, GR, G6PD and GSH.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

102.14

Formula

C₄H₁₀N₂O

CAS No.

55-18-5

Appearance

Liquid (Density: 0.95 g/cm³)

Color

Colorless to light yellow

SMILES

CCN(CC)N=O

Structure Classification

Others

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (979.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (979.05 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	9.7905 mL	48.9524 mL	97.9048 mL
5 mM	1.9581 mL	9.7905 mL	19.5810 mL
10 mM	0.9790 mL	4.8952 mL	9.7905 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (24.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (24.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (24.48 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (979.05 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.98%

Select Batch:

Data Sheet (277 KB) SDS (420 KB)

COA (245 KB) HNMR (242 KB) MS (230 KB) GC (243 KB)

Handling Instructions (2659 KB)

References

[1]. Ramakrishnan G, et al. Suppression of N-nitrosodiethylamine induced hepatocarcinogenesis by silymarin in rats. Chem Biol Interact. 2006 Jun 10;161(2):104-14. [Content Brief]

[2]. Bansal AK, et al. Protective role of Vitamin E pre-treatment on N-nitrosodiethylamine induced oxidative stress in rat liver. Chem Biol Interact. 2005 Oct 20;156(2-3):101-11. [Content Brief]

[3]. Gombar CT, et al. Pharmacokinetics of N-nitrosodimethylamine in beagles. Cancer Res. 1987 Jan 15;47(2):343-7. [Content Brief]

[4]. Verna L, et al. N-nitrosodiethylamine mechanistic data and risk assessment: bioactivation, DNA-adduct formation, mutagenicity, and tumor initiation. Pharmacol Ther. 1996;71(1-2):57-81. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	9.7905 mL	48.9524 mL	97.9048 mL	244.7621 mL
	5 mM	1.9581 mL	9.7905 mL	19.5810 mL	48.9524 mL
	10 mM	0.9790 mL	4.8952 mL	9.7905 mL	24.4762 mL
	15 mM	0.6527 mL	3.2635 mL	6.5270 mL	16.3175 mL
	20 mM	0.4895 mL	2.4476 mL	4.8952 mL	12.2381 mL
	25 mM	0.3916 mL	1.9581 mL	3.9162 mL	9.7905 mL
	30 mM	0.3263 mL	1.6317 mL	3.2635 mL	8.1587 mL
	40 mM	0.2448 mL	1.2238 mL	2.4476 mL	6.1191 mL
	50 mM	0.1958 mL	0.9790 mL	1.9581 mL	4.8952 mL
	60 mM	0.1632 mL	0.8159 mL	1.6317 mL	4.0794 mL
	80 mM	0.1224 mL	0.6119 mL	1.2238 mL	3.0595 mL
	100 mM	0.0979 mL	0.4895 mL	0.9790 mL	2.4476 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N-Nitrosodiethylamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Nitrosodiethylamine55-18-5Diethylnitrosamine DEN DENADNA/RNA SynthesisInhibitorinhibitorinhibit

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N-Nitrosodiethylamine (Synonyms: Diethylnitrosamine; DEN; DENA)

Customer Review

Other Forms of N-Nitrosodiethylamine:

N-Nitrosodiethylamine Related Antibodies

6 Publications Citing Use of MCE N-Nitrosodiethylamine

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All DNA/RNA Synthesis Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

N-Nitrosodiethylamine Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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