Oleana-2,12-dien-28-oic acid

Name: Oleana-2,12-dien-28-oic acid
Brand: MedChemExpress
SKU: HY-148201
Rating: 4.5 (1 reviews)

Cat. No.: HY-148201 Purity: 99.88%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Oleana-2,12-dien-28-oic acid is an HBV-DNA inhibitor, HBsAg and HBeAg inhibitor. Oleana-2,12-dien-28-oic acid can be used in hepatitis B virus infection disease research.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Oleana-2,12-dien-28-oic acid 화학구조

CAS No. : 272108-04-0

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	견적 받기
100 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 publication(s) in Google Scholar

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
References
고객리뷰

제품 설명

Oleana-2,12-dien-28-oic acid is an HBV-DNA inhibitor, HBsAg and HBeAg inhibitor. Oleana-2,12-dien-28-oic acid can be used in hepatitis B virus infection disease research^[1].

In Vitro

Oleana-2,12-dien-28-oic acid (4-100 μg/mL; 7 d) shows the inhibition on hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) secreted by HepG2.2.15 cells^[1].
Oleana-2,12-dien-28-oic acid (4-100 μg/mL; 7 d) inhibits the replication of hepatitis B virus deoxyribonucleic acid (HBV-DNA) of HepG2.2.15 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	HepG2 .2 .15 cells
Concentration:	4, 20, 100 μg/mL
Incubation Time:	7 days
Result:	Inhibited hepatitis B surface antigen (HBsAg) of 60.5%, 69.8%, and 85.3% at 4, 20, and 100 μg/mL, respectively. Inhibited hepatitis B e antigen (HBeAg) of 45.1%, 60.5%, and 76.9% at 4, 20, and 100 μg/mL, respectively.

분자량

438.69

화학식

C₃₀H₄₆O₂

CAS No.

272108-04-0

Appearance

Solid

Color

White to off-white

SMILES

C[C@@]12C([C@@]3([H])[C@@](CC2)(CCC(C)(C3)C)C(O)=O)=CC[C@@]4([H])[C@]1(CC[C@]5([H])[C@@]4(CC=CC5(C)C)C)C

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (227.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2795 mL	11.3976 mL	22.7951 mL
5 mM	0.4559 mL	2.2795 mL	4.5590 mL
10 mM	0.2280 mL	1.1398 mL	2.2795 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.88%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (253 KB) HNMR (261 KB) RP-HPLC (205 KB) MS (72 KB)

Handling Instructions (2659 KB)

References

[1]. Wu XM, et al. Medical application of A-ring double-bond oleanolic acid in preparation of medicines for preventing and treating viral hepatitis B. CN111759846A.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.2795 mL	11.3976 mL	22.7951 mL	56.9878 mL
	5 mM	0.4559 mL	2.2795 mL	4.5590 mL	11.3976 mL
	10 mM	0.2280 mL	1.1398 mL	2.2795 mL	5.6988 mL
	15 mM	0.1520 mL	0.7598 mL	1.5197 mL	3.7992 mL
	20 mM	0.1140 mL	0.5699 mL	1.1398 mL	2.8494 mL
	25 mM	0.0912 mL	0.4559 mL	0.9118 mL	2.2795 mL
	30 mM	0.0760 mL	0.3799 mL	0.7598 mL	1.8996 mL
	40 mM	0.0570 mL	0.2849 mL	0.5699 mL	1.4247 mL
	50 mM	0.0456 mL	0.2280 mL	0.4559 mL	1.1398 mL
	60 mM	0.0380 mL	0.1900 mL	0.3799 mL	0.9498 mL
	80 mM	0.0285 mL	0.1425 mL	0.2849 mL	0.7123 mL
	100 mM	0.0228 mL	0.1140 mL	0.2280 mL	0.5699 mL