2. Immunology/Inflammation Apoptosis NF-κB Metabolic Enzyme/Protease
  3. SOD Ferroptosis Keap1-Nrf2 Heme Oxygenase (HO) Glutathione Peroxidase
  4. Picein

Picein is an antioxidant and anti-inflammatory agent. Picein can be isolated from the leaves of Picrorhiza kurroa. Picein reduces MDA levels and increases the levels of SOD, GPX and TAC. Picein alleviates oxidative stress and promotes bone regeneration in osteoporotic bone defects by inhibiting Ferroptosis (via activation of the Nrf2/HO-1/GPX4 pathway). Picein prevents scopolamine (HY-N0296)-induced passive avoidance memory impairment in rats. Picein can be used in research related to osteoporotic bone defects and Alzheimer's disease.

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Picein

Picein Chemical Structure

CAS No. : 530-14-3

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Picein:

Picein Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Picein

View All Heme Oxygenase (HO) Isoform Specific Products:

View All Isoforms
HO-1 HO-2

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GPX1 GPX4

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Picein is an antioxidant and anti-inflammatory agent. Picein can be isolated from the leaves of Picrorhiza kurroa. Picein reduces MDA levels and increases the levels of SOD, GPX and TAC. Picein alleviates oxidative stress and promotes bone regeneration in osteoporotic bone defects by inhibiting Ferroptosis (via activation of the Nrf2/HO-1/GPX4 pathway). Picein prevents scopolamine (HY-N0296)-induced passive avoidance memory impairment in rats. Picein can be used in research related to osteoporotic bone defects and Alzheimer's disease[1][2][3].

IC50 & Target[1]

HO-1

 

GPX4

 

Cellular Effect
Cell Line Type Value Description References
HepG2 2.2.15 CC50
> 3947.9 μM
Compound: 2
Cytotoxicity against human HepG 2.2.15 cells by MTT assay
Cytotoxicity against human HepG 2.2.15 cells by MTT assay
[PMID: 25737008]
HepG2 2.2.15 IC50
227.8 μM
Compound: 2
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as decrease in viral DNA replication treated for 7 days by real time PCR analysis
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as decrease in viral DNA replication treated for 7 days by real time PCR analysis
[PMID: 25737008]
HepG2 2.2.15 IC50
> 3947.9 μM
Compound: 2
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBeAg secretion after 72 hrs by ELISA
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBeAg secretion after 72 hrs by ELISA
[PMID: 25737008]
HepG2 2.2.15 IC50
> 3947.9 μM
Compound: 2
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 72 hrs by ELISA
Antiviral activity against hepatitis B viral in human HepG2.2.15 cells assessed as surface antigen HBsAg secretion after 72 hrs by ELISA
[PMID: 25737008]
In Vitro

Picein (10-80 μM; 24 h) shows no toxicity to rat bone marrow mesenchymal stem cells (BMSCs), and Picein (80 μM; 2 h pretreatment followed by 24, 48, 72 h erastin incubation) significantly restores the viability of rat BMSCs impaired by Erastin[1].
Picein (80 μM) significantly restores the viability of HUVECs impaired by Erastin[1].
Picein (80 μM; 48 h) significantly promotes M2 polarization of Raw 264.7 macrophages and reverses the inhibitory effect of 5 μM erastin[1].
Picein (for 7-21 days) significantly reverses the inhibitory effect of 5 μM erastin on osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs), which is verified by increased alkaline phosphatase (ALP) activity, enhanced mineralization level, and upregulated expression of osteogenesis-related proteins and genes[1].
Picein reduces ROS levels and restores mitochondrial function in neuroblastoma SH-SY5Y cells treated with Menadione (HY-B0332), indicating its potential as a neuroprotective agent[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Picein Related Antibodies
In Vivo

Picein (40-80 mg/kg; topical to femoral condyle; daily; 4 weeks) promotes bone regeneration in ovariectomized rats with osteoporotic femoral condyle defects, with the 80 mg/kg dose yielding a 48% BV/TV, and reduces local and systemic inflammation by lowering IL-1β, IL-6, and TNF-α levels[1].
Picein (1.25-5 mg/kg; i.c.v.; daily; 7 days), most potently at 2.5 mg/kg, reduces hippocampal oxidative stress, increases neuronal density, and reverses scopolamine-induced passive avoidance memory impairment in male Wistar rats[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

298.29

Formula

C14H18O7
CAS No.
Appearance

Solid

Color

Off-white to light yellow

SMILES

O[C@H]([C@H]([C@@H]([C@H](O1)CO)O)O)[C@@H]1OC2=CC=C(C=C2)C(C)=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 200 mg/mL (670.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3524 mL 16.7622 mL 33.5244 mL
5 mM 0.6705 mL 3.3524 mL 6.7049 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 5 mg/mL (16.76 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 5 mg/mL (16.76 mM); Clear solution

    This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.3524 mL 16.7622 mL 33.5244 mL 83.8111 mL
5 mM 0.6705 mL 3.3524 mL 6.7049 mL 16.7622 mL
10 mM 0.3352 mL 1.6762 mL 3.3524 mL 8.3811 mL
15 mM 0.2235 mL 1.1175 mL 2.2350 mL 5.5874 mL
20 mM 0.1676 mL 0.8381 mL 1.6762 mL 4.1906 mL
25 mM 0.1341 mL 0.6705 mL 1.3410 mL 3.3524 mL
30 mM 0.1117 mL 0.5587 mL 1.1175 mL 2.7937 mL
40 mM 0.0838 mL 0.4191 mL 0.8381 mL 2.0953 mL
50 mM 0.0670 mL 0.3352 mL 0.6705 mL 1.6762 mL
60 mM 0.0559 mL 0.2794 mL 0.5587 mL 1.3969 mL
80 mM 0.0419 mL 0.2095 mL 0.4191 mL 1.0476 mL
100 mM 0.0335 mL 0.1676 mL 0.3352 mL 0.8381 mL
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Picein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Picein530-14-3SODFerroptosisKeap1-Nrf2Heme Oxygenase (HO)Glutathione PeroxidaseSuperoxide DismutaseHeme Oxygenaseendothelial cellsosteoporotic bone defect rat modelsscopolamine-injured ratsbone marrow mesenchymal stem cellsSH-SY5Y cellsM2 macrophagesferroptosisHUVECsRaw 264.7 macrophagesNrf2/HO-1/GPX4 pathwayInhibitorinhibitorinhibit

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