Cloning and characterization of human histone deacetylase 8

  • FEBS Lett. 2000 Jul 28;478(1-2):77-83. doi: 10.1016/s0014-5793(00)01813-5.
I Van den Wyngaert  1 ,  W de Vries ,  A Kremer ,  J Neefs ,  P Verhasselt ,  W H Luyten ,  S U Kass
Affiliations
  • 1. Department of Advanced Bio-Technologies, Jansen Research Foundation, Turnhoutseweg 30, 2340 Beerse, Belgium.
PMID: 10922473 DOI: 10.1016/s0014-5793(00)01813-5
Abstract

To date, seven different human histone deacetylases (HDACs) have been identified, which fall into two distinct classes. We have isolated and characterized a cDNA encoding a novel human HDAC, which we name HDAC8. HDAC8 shows a high degree of sequence similarity to HDAC1 and HDAC2 and thus belongs to the class I of HDACs. HDAC8 is expressed in a variety of tissues. Human cells overexpressing HDAC8 localize the protein in sub-nuclear compartments whereas HDAC1 shows an even nuclear distribution. In addition, the HDAC8 gene is localized on the X chromosome at position q13, which is close to the XIST gene and chromosomal breakpoints associated with preleukemia.