Quinine (hydrochloride)

Name: Quinine (hydrochloride)
Brand: MedChemExpress
SKU: HY-110082
Rating: 5.0 (7 reviews)

Cat. No.: HY-110082 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Quinine hydrochloride is an alkaloid extracted from cinchona bark and exhibits oral activity, acting as a potassium channel inhibitor. Quinine hydrochloride modulates the tolerance of red blood cells and presents dose-dependent toxicity and embryonic effects. Quinine hydrochloride is a typical hemolysin that directly lyses red blood cells, with cellular components of red blood cell membranes as its action targets. Quinine hydrochloride disrupts red blood cell membranes and induces hemolysis at high concentrations, while merely weakening the anti-hemolytic capacity of red blood cells at low concentrations. Quinine hydrochloride continuously reduces red blood cell tolerance after in vivo administration, and high doses can also alter blood cell counts. Quinine hydrochloride can be applied to researches related to red blood cell hemolysis, cancer and malaria.

For research use only. We do not sell to patients.

Quinine (hydrochloride) Chemical Structure

CAS No. : 130-89-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
50 g	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Quinine (hydrochloride):

Quinine In-stock

7 Publications Citing Use of MCE Quinine (hydrochloride)

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Quinine hydrochloride acts as a simple hemolysin on rabbit erythrocytes; pretreatment of rabbit erythrocytes reduces their resistance to the lytic effects of saponin and sodium taurocholate in vitro^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Quinine hydrochloride (25-250 mg/kg per day; oral administration; daily dosing for 4 consecutive weeks) reduces food intake and body weight in rats, alters spleen weight in male rats, and decreases kidney and adrenal gland weights in female rats^[3].
Quinine hydrochloride (administered orally daily at doses of 1-200 mg/kg for 13 consecutive weeks) causes dose-dependent decreases in body weight and food intake, as well as reversible biochemical changes^[3].
Quinine hydrochloride (60-120 mg/kg per day; p.o.; daily administration; for 13 consecutive weeks) causes reversible inhibition of body weight gain, reduced food intake, and transient increases in kidney weight in male rats at doses ≥85 mg/kg per day^[3].
Quinine hydrochloride (85-200 mg/kg per day; p.o.; daily administration; for 13 consecutive weeks) does not induce ototoxic effects or hearing impairment^[3].
Quinine hydrochloride (50-200 mg/kg per day; p.o.; daily administration; gestational days 6-15) is orally administered to mated Sprague-Dawley rats on gestational days 6-15. Maternal toxicity occurs at doses ≥100 mg/kg, while mild fetal effects (decreased body weight, increased incidence of malformations) are observed at a dose of 200 mg/kg^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Sprague-Dawley (male and female)^[3]
Dosage:	25 mg/kg per day; 100 mg/kg per day; 200 mg/kg per day; 250 mg/kg per day
Administration:	p.o.; daily; 4 weeks
Result:	Decreased food intake and body weight at 100, 200 and 250 mg/kg daily. Slightly impaired food utilization at the end of the study. Suppressed weight gain in females at 25 mg/kg daily. Elevated spleen weight in all treated male rats. Lowered kidney and adrenal weights in all treated female rats.

Animal Model:	Sprague-Dawley (male and female)^[3]
Dosage:	1 mg/kg per day; 10 mg/kg per day; 40 mg/kg per day; 100 mg/kg per day; 200 mg/kg per day
Administration:	p.o.; daily; 13 weeks
Result:	Retarded body weight gain at 100 and 200 mg/kg daily. Decreased food intake and utilization at 200 mg/kg daily. Accelerated weight gain in the 200 mg/kg group during recovery. Raised plasma urea across genders at 100 and 200 mg/kg daily. Elevated serum inorganic phosphorus in females at 40, 100 and 200 mg/kg daily. Lowered total protein in females at 100 and 200 mg/kg daily. Reversed all biochemical alterations in the recovery period. Aggravated periportal glycogen depletion in females at 100 and 200 mg/kg daily. Produced no changes in organ weight, vision or hearing function.

Animal Model:	Sprague-Dawley (male and female)^[3]
Dosage:	60 mg/kg per day; 85 mg/kg per day; 120 mg/kg per day
Administration:	p.o.; daily; 13 weeks
Result:	Recorded no drug-related deaths. Induced mild alopecia since week 3 at 120 mg/kg daily. Retarded weight gain and food intake at 85/120 mg/kg daily, reversible during recovery. Elevated phosphorus in females at 120 mg/kg daily, with partial persistence. Increased kidney weight in males at 85/120 mg/kg daily, which recovered later. Caused no abnormalities in blood, urine, ophthalmic and histological examinations.

Animal Model:	Sprague-Dawley (time-mated female)^[3]
Dosage:	50 mg/kg per day; 100 mg/kg per day; 200 mg/kg per day
Administration:	p.o.; daily; gestation days 6-15
Result:	Induced salivation, partial alopecia, excessive water intake and mild growth retardation in maternal rats at 100 and 200 mg/kg daily. Exerted no impacts on litter size, embryo survival and foetal malformation rate across all doses. Remarkably lowered average foetal weight at the 200 mg/kg daily dose. Elevated the rates of visceral anomalies, skeletal anomalies and sternebrae variations in foetuses at 200 mg/kg daily.

Molecular Weight

360.88

Formula

C₂₀H₂₅ClN₂O₂

CAS No.

130-89-2

Appearance

Solid

Color

White to off-white

SMILES

O[C@H](C1=CC=NC2=C1C=C(OC)C=C2)[C@@]3([H])[N@](C[C@@H]4C=C)CC[C@H]4C3.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

RT, stored under nitrogen

In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (277.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7710 mL	13.8550 mL	27.7100 mL
5 mM	0.5542 mL	2.7710 mL	5.5420 mL
10 mM	0.2771 mL	1.3855 mL	2.7710 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.93 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: 2.5 mg/mL (6.93 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (283 KB) SDS (394 KB)

COA (254 KB) HNMR (121 KB) RP-HPLC (101 KB) MS (145 KB)

Handling Instructions (2659 KB)

References

[1]. Ponder E, et al. Effect of Quinine Hydrochloride on Resistance of Rabbit Red Cells. [Journal Name]. 1936 Mar;34(2).

[2]. Muhtadi FJ, et al. Quinine Hydrochloride. Anal Profiles Drug Subst. 1983;12:547-621.

[3]. Colley JC, et al. Toxicity studies with quinine hydrochloride. Toxicology. 1989 Feb;54(2):219-226.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7710 mL	13.8550 mL	27.7100 mL	69.2751 mL
	5 mM	0.5542 mL	2.7710 mL	5.5420 mL	13.8550 mL
	10 mM	0.2771 mL	1.3855 mL	2.7710 mL	6.9275 mL
	15 mM	0.1847 mL	0.9237 mL	1.8473 mL	4.6183 mL
	20 mM	0.1386 mL	0.6928 mL	1.3855 mL	3.4638 mL
	25 mM	0.1108 mL	0.5542 mL	1.1084 mL	2.7710 mL
	30 mM	0.0924 mL	0.4618 mL	0.9237 mL	2.3092 mL
	40 mM	0.0693 mL	0.3464 mL	0.6928 mL	1.7319 mL
	50 mM	0.0554 mL	0.2771 mL	0.5542 mL	1.3855 mL
	60 mM	0.0462 mL	0.2309 mL	0.4618 mL	1.1546 mL
	80 mM	0.0346 mL	0.1732 mL	0.3464 mL	0.8659 mL
	100 mM	0.0277 mL	0.1386 mL	0.2771 mL	0.6928 mL