2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras
  4. Rasarfin

Rasarfin is a dual Ras and ARF6 inhibitor.

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Rasarfin

Rasarfin Estructura química

No. CAS : 674359-73-0

Tamaño Precio Stock Cantidad
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
1 mg En stock
5 mg En stock
10 mg En stock
50 mg En stock
100 mg En stock
200 mg   Obtener un presupuesto  
500 mg   Obtener un presupuesto  

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Revisión del cliente

Based on 1 publication(s) in Google Scholar

Rasarfin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE Rasarfin

Ver todos los productos específicos de isoformas Ras:

Ver todas las isoformas
K-Ras H-Ras N-Ras Ras

  • Actividad biológica

  • Pureza y Documentación

  • Referencias

  • Revisión del cliente

Descripciòn

Rasarfin is a dual Ras and ARF6 inhibitor[1].
In Vitro

Rasarfin potently inhibits agonist-induced ERK1/2 signaling by GPCRs, and MAPK and Akt signaling by EGFR, as well as prevents cancer cell proliferation[1].
? Rasarfin blocks GPCR internalization through inhibition of ARF6[1].
? Rasarfin treatment results in a dose-dependent reduction in MDA-MB-231 cells[1].
? Rasarfin inhibits the recruitment of the clathrin adaptor AP-2 to β-arrestin2 and receptor internalization, both processes that require active ARF6[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Rasarfin Related Antibodies
Peso molecular

425.91

Fòrmula

C23H24ClN3O3
No. CAS
Appearance

Solid

Color

White to yellow

SMILES

O=C(C1=CC2=C(C=CC=C2)O1)NC3=CC=C(N4CCN(CC4)C(C(C)C)=O)C(Cl)=C3
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvente y solubilidad
In Vitro: 

DMSO : 38.89 mg/mL (91.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3479 mL 11.7396 mL 23.4791 mL
5 mM 0.4696 mL 2.3479 mL 4.6958 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Calculadora de molaridad

  • Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.87 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.88 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación

Purity: 99.57%

SDS (251 KB)

COA (245 KB) HNMR (278 KB) LCMS (94 KB)

Instrucciones de manejo (2659 KB)
Referencias

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3479 mL 11.7396 mL 23.4791 mL 58.6978 mL
5 mM 0.4696 mL 2.3479 mL 4.6958 mL 11.7396 mL
10 mM 0.2348 mL 1.1740 mL 2.3479 mL 5.8698 mL
15 mM 0.1565 mL 0.7826 mL 1.5653 mL 3.9132 mL
20 mM 0.1174 mL 0.5870 mL 1.1740 mL 2.9349 mL
25 mM 0.0939 mL 0.4696 mL 0.9392 mL 2.3479 mL
30 mM 0.0783 mL 0.3913 mL 0.7826 mL 1.9566 mL
40 mM 0.0587 mL 0.2935 mL 0.5870 mL 1.4674 mL
50 mM 0.0470 mL 0.2348 mL 0.4696 mL 1.1740 mL
60 mM 0.0391 mL 0.1957 mL 0.3913 mL 0.9783 mL
80 mM 0.0293 mL 0.1467 mL 0.2935 mL 0.7337 mL
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Rasarfin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Rasarfin674359-73-0RasGPCRinternalizationTNBCtriplenegativebreastcancerInhibitorinhibitorinhibit

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Nombre del producto:
Rasarfin
Cat. No.:
HY-139950
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SAFETY DATA SHEET (SDS)

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