1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-2
  5. ENPP-2 Protein, Mouse (HEK293, His)

ENPP-2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P75733
SDS COA Handling Instructions

The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA).It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement.ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-His, N-10*His labeled tag.

For research use only. We do not sell to patients.

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5 μg $53 In-stock
10 μg $89 In-stock
50 μg $250 In-stock
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Description

The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA).It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement.ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-His, N-10*His labeled tag.

Background

The ENPP-2 Protein hydrolyzes lysophospholipids, specifically lysophosphatidylcholine, to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. It can also act on sphingosylphosphorylcholine, producing sphingosine-1-phosphate, which modulates cell motility. In addition, ENPP-2 Protein has been shown to hydrolyze bis-pNPP, pNP-TMP, and ATP to varying extents. It is involved in various motility-related processes, including angiogenesis and neurite outgrowth, and acts as an angiogenic factor by stimulating the migration of smooth muscle cells and microtubule formation. ENPP-2 Protein is also implicated in melanoma cell migration, potentially through a pertussis toxin-sensitive G protein. It may play a role in parturition induction, as well as in cell proliferation and adipose tissue development. Furthermore, it is required for LPA production in activated platelets and cleaves sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. ENPP-2 Protein exhibits a preference for the sn-1 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).

Species

Mouse

Source

HEK293

Tag

N-His;N-10*His

Accession

Q9R1E6-1 (S49-I862)

Gene ID
Molecular Construction
N-term
His
ENPP-2 (S49-I862)
Accession # Q9R1E6-1
C-term
Synonyms
Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; Autotaxin; LysoPLD; Npps2; Pdnp2
AA Sequence

SPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEI

Molecular Weight

Approximately 100 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ENPP-2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ENPP-2 Protein, Mouse (HEK293, His)
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HY-P75733
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