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ENPP-2 Protein, Mouse (HEK293, His)

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The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA).It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement.ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for ENPP-2 Protein, Mouse (HEK293, His)
  • ENPP-2 Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA).It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement.ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-10*His labeled tag.

Background

The ENPP-2 Protein hydrolyzes lysophospholipids, specifically lysophosphatidylcholine, to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. It can also act on sphingosylphosphorylcholine, producing sphingosine-1-phosphate, which modulates cell motility. In addition, ENPP-2 Protein has been shown to hydrolyze bis-pNPP, pNP-TMP, and ATP to varying extents. It is involved in various motility-related processes, including angiogenesis and neurite outgrowth, and acts as an angiogenic factor by stimulating the migration of smooth muscle cells and microtubule formation. ENPP-2 Protein is also implicated in melanoma cell migration, potentially through a pertussis toxin-sensitive G protein. It may play a role in parturition induction, as well as in cell proliferation and adipose tissue development. Furthermore, it is required for LPA production in activated platelets and cleaves sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. ENPP-2 Protein exhibits a preference for the sn-1 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag N-10*His
  • Accession

    Q9R1E6-1 (S49-I862)

  • Gene ID
    18606  [NCBI]
  • Molecular Construction
    • N-term
    • 10*His
    • ENPP-2 (S49-I862)
      Accession # Q9R1E6-1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ENPP2; LysoPLD; Prev. PDNP2; Phosphodiesterase I/Nucleotide Pyrophosphatase 2; Autotaxin; Plasma Lysophospholipase D; ATX; Alternative Protein ENPP2; Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2; Autotaxin-T; Extracellular Lysophosphol

  • AA Sequence

    SPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEI

  • Molecular Weight

    Approximately 55-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • ENPP-2 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ENPP-2 Protein, Mouse (HEK293, His)
  • ENPP-2 Protein, Mouse (HEK293, His) Product Image