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Klotho beta Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Klotho beta Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Klotho beta Protein, Mouse (HEK293, His)
  • Klotho beta Protein, Mouse (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Klotho beta protein represses cholesterol 7-alpha-hydroxylase (CYP7A1), a key enzyme in bile acid synthesis. It enhances binding between fibroblast growth factor receptors and fibroblast growth factor 21, and directly interacts with FGF19 and FGF21. This positions Klotho beta as a crucial modulator of metabolic and growth factor signaling pathways. Klotho beta Protein, Mouse (HEK293, His) is the recombinant mouse-derived Klotho beta protein, expressed by HEK293 , with C-10*His labeled tag.

Background

Klotho beta, a multifunctional protein, plays a role in the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), a pivotal enzyme in bile acid synthesis. While its glycosidase activity is likely inactive, Klotho beta demonstrates its molecular versatility by enhancing the binding affinity between fibroblast growth factor receptors (FGFR1 and FGFR4) and fibroblast growth factor 21 (FGF21). The protein also engages in direct interactions with FGF19 and FGF21 through its C-terminus, highlighting its involvement in regulatory pathways associated with metabolic processes and growth factor signaling. This intricate network of interactions positions Klotho beta as a key player in modulating crucial physiological functions.

Verified Bioactivity

Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED50 for this effect is 291.3 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q99N32 (F53-L995)

  • Gene ID
    83379  [NCBI]
  • Molecular Construction
    • N-term
    • Klotho beta (F53-L995)
      Accession # Q99N32
    • 10*His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    KLB; B-Klotho; Klotho Beta; BKL; Beta-Klotho; Klotho Beta Like; BetaKlotho; KLB Protein; Klotho Beta-Like Protein

  • AA Sequence

    FSGDGKAIWDKKQYVSPVNPSQLFLYDTFPKNFSWGVGTGAFQVEGSWKTDGRGPSIWDRYVYSHLRGVNGTDRSTDSYIFLEKDLLALDFLGVSFYQFSISWPRLFPNGTVAAVNAQGLRYYRALLDSLVLRNIEPIVTLYHWDLPLTLQEEYGGWKNATMIDLFNDYATYCFQTFGDRVKYWITIHNPYLVAWHGFGTGMHAPGEKGNLTAVYTVGHNLIKAHSKVWHNYDKNFRPHQKGWLSITLGSHWIEPNRTDNMEDVINCQHSMSSVLGWFANPIHGDGDYPEFMKTGAMIPEFSEAEKEEVRGTADFFAFSFGPNNFRPSNTVVKMGQNVSLNLRQVLNWIKLEYDDPQILISENGWFTDSYIKTEDTTAIYMMKNFLNQVLQAIKFDEIRVFGYTAWTLLDGFEWQDAYTTRRGLFYVDFNSEQKERKPKSSAHYYKQIIQDNGFPLKESTPDMKGRFPCDFSWGVTESVLKPEFTVSSPQFTDPHLYVWNVTGNRLLYRVEGVRLKTRPSQCTDYVSIKKRVEMLAKMKVTHYQFALDWTSILPTGNLSKVNRQVLRYYRCVVSEGLKLGVFPMVTLYHPTHSHLGLPLPLLSSGGWLNMNTAKAFQDYAELCFRELGDLVKLWITINEPNRLSDMYNRTSNDTYRAAHNLMIAHAQVWHLYDRQYRPVQHGAVSLSLHCDWAEPANPFVDSHWKAAERFLQFEIAWFADPLFKTGDYPSVMKEYIASKNQRGLSSSVLPRFTAKESRLVKGTVDFYALNHFTTRFVIHKQLNTNRSVADRDVQFLQDITRLSSPSRLAVTPWGVRKLLAWIRRNYRDRDIYITANGIDDLALEDDQIRKYYLEKYVQEALKAYLIDKVKIKGYYAFKLTEEKSKPRFGFFTSDFRAKSSVQFYSKLISSSGLPAENRSPACGQPAEDTDCTICSFLVEKKPL

  • Predicted Molecular Mass

    110.3 kDa

  • Molecular Weight

    Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 10% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 90%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human FGF21 Protein at 2 μg/mL (100μL/well) can bind Mouse Klotho beta. The ED50 for this effect is 291.3 ng/mL.

  • Klotho beta Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Klotho beta Protein, Mouse (HEK293, His)
  • Experimental Validation Results for Klotho beta Protein, Mouse (HEK293, His)
  • Klotho beta Protein, Mouse (HEK293, His) Product Image