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Orm2 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

Orm2 Protein, Human (HEK293, His) produced in HEK293 cells, is a recombinant human Alpha-1-acid glycoprotein 2 with His tag. Alpha-1-acid glycoprotein 2 belongs to the immunocalin family, which is a lipocalin subfamily with immunomodulatory functions.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

Orm2 Protein, Human (HEK293, His) produced in HEK293 cells, is a recombinant human Alpha-1-acid glycoprotein 2 with His tag. Alpha-1-acid glycoprotein 2 belongs to the immunocalin family, which is a lipocalin subfamily with immunomodulatory functions[1].

Background

Alpha-1-acid glycoprotein 2 (Orosomucoid, ORM) is an acute-phase protein that belongs to the immunocalin subfamily, a group of small-molecule-binding proteins with immunomodulatory functions. Alpha-1-acid glycoprotein 2 exerts anti-inflammatory effects by modulating microglial activation and migration during brain inflammation[1].

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P19652 (Q19-S201)

  • Gene ID
    5005  [NCBI]
  • Molecular Construction
    • N-term
    • Orm2 (Q19-S201)
      Accession # P19652
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    ORM2; Alpha-1-Acid Glycoprotein, Type 2; Orosomucoid 2; Alpha-1-Acid Glycoprotein 2; AGP2; AGP 2; AGP-B'; OMD 2; AGP-B; Orosomucoid-2

  • AA Sequence

    QIPLCANLVPVPITNATLDRITGKWFYIASAFRNEEYNKSVQEIQATFFYFTPNKTEDTIFLREYQTRQNQCFYNSSYLNVQRENGTVSRYEGGREHVAHLLFLRDTKTLMFGSYLDDEKNWGLSFYADKPETTKEQLGEFYEALDCLCIPRSDVMYTDWKKDKCEPLEKQHEKERKQEEGES

  • Molecular Weight

    Approximately 25-50 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Myungjin Jo, et al. Astrocytic Orosomucoid-2 Modulates Microglial Activation and Neuroinflammation. J Neurosci. 2017 Mar 15;37(11):2878-2894. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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