VLDLR Protein, Mouse (HEK293, His)

VLDLR protein is a multifunctional cell surface receptor that plays a crucial role in energy metabolism by binding VLDL and promoting cellular uptake through endocytosis. In addition to lipid transport, VLDLR has broad binding affinities for molecules such as Reelin/RELN, APOE, and CLU. VLDLR Protein, Mouse (HEK293, His) is the recombinant mouse-derived VLDLR protein, expressed by HEK293 , with C-His labeled tag. The total length of VLDLR Protein, Mouse (HEK293, His) is 770 a.a., with molecular weight of 110-130 kDa.

The VLDLR protein operates as a multifunctional cell surface receptor pivotal in energy metabolism, particularly by binding VLDL and facilitating its cellular uptake through endocytosis. Beyond its role in lipid transport, VLDLR exhibits a broad binding affinity for various molecules, including Reelin/RELN, apolipoprotein E/APOE-containing ligands, and clusterin/CLU. In the inactive state of the pathway, VLDLR forms homo or heterooligomers with LRP8. Upon ligand binding, these homooligomers rearrange into higher-order receptor clusters, transducing the extracellular RELN signal to intracellular signaling processes through DAB1 binding on its cytoplasmic tail. This interaction triggers DAB1 phosphorylation, orchestrating the cell responses crucial for the correct positioning of newly generated neurons. Additionally, VLDLR serves as a stop signal for migrating neurons, preventing entry into the marginal zone. Interactions with various proteins, including LDLRAP1, SNX17, PCSK9, PAFAH1B3, PAFAH1B2, STX5, and CLU, further highlight the intricate regulatory network of VLDLR in cellular processes.

Mouse VLDLR, His Tag immobilized on CM5 Chip can bind Mouse PCSK9, His Tag with an affinity constant of 0.28 nM as determined in SPR assay (Biacore T200).

Mouse

HEK293

C-His

P98156 (G28-S797)

22359  [NCBI]

GKKAKCDSSQFQCTNGRCITLLWKCDGDEDCADGSDEKNCVKKTCAESDFVCKNGQCVPNRWQCDGDPDCEDGSDESPEQCHMRTCRINEISCGARSTQCIPVSWRCDGENDCDNGEDEENCGNITCSADEFTCSSGRCVSRNFVCNGQDDCDDGSDELDCAPPTCGAHEFQCSTSSCIPLSWVCDDDADCSDQSDESLEQCGRQPVIHTKCPTSEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRPDQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDMSKVCDQEQDCRDWSDEPLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCECSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQKAIFSASIDDKVGRHFKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGAKRKFLFNSDLREPASIAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTEDIQWPNGITLDLVKSRLYWLDSKLHMLSSVDLNGQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQPSGKNWCEDDMENGGCEYLCLPAPQINDHSPKYTCSCPNGYNLEENGRECQSTSTPVTYSETKDINTTDILRTSGLVPGGINVTTAVSEVSVPPKGTS

110-130 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.