S3D5 

S3D5 is a highly efficient and selective PROTAC degrader targeting STAT3 (KD = 4.35 μM). S3D5 induces degradation of STAT3 in HepG2 cells without significant effects on other STAT proteins. S3D5 exhibits good anti-hepatocellular carcinoma cell proliferation activity, which can be explained by activating the p53 pathway. S3D5 degradation of the STAT3 protein is mediated by the ubiquitin–proteasome system (UPS). S3D5 can be used for the study of hepatocellular carcinoma^[1]. (Pink: Ligands for Target Protein for PROTAC ligand (HY-163526); Blue: Ligands for E3 Ligase ligand (HY-10984); Black: linker (HY-130447)).

S3D5 (4-20 μM, 96 h) shows optimal activity in HepG2 cells, with an IC₅₀ value of 8.53 μM^[1].
S3D5 (0.1-2 μM, 24-48 h) can induce STAT3 degradation in HepG2 cells (DC₅₀ = 110 nM), and have little effect at any concentration on STAT1, STAT2, STAT4, STAT5, and STAT6 proteins^[1].
S3D5 (2.5-20 μM, 96 h) inhibits the growth of HepG2 cells in a dose-dependent manner and inhibits the proliferation of 293T cells at 5 μM^[1].
S3D5 (1-20 μM, 14 days) leads to a significant reduction in both the number and size of HepG2 cell colonies compared to the control^[1].
S3D5 (5μM, 24-72 h) can inhibit the proliferation, migration and invasion of HepG2 cells^[1].
S3D5 (0.1-2 μM, 48 h) dose-dependently upregulates p53 and p21 protein levels, while downregulates STAT3 protein levels in HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

956.89

C₄₁H₄₅F₅N₆O₁₃S

3033746-21-0

CN(S(=O)(C1=C(C(F)=C(C(F)=C1F)F)F)=O)CC(NC2=CC=C(C=C2)C(NCCOCCOCCOCCOCCOCCNC3=C4C(C(N(C5C(NC(CC5)=O)=O)C4=O)=O)=CC=C3)=O)=O

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• [1]. Wang K, et al. Discovery of a potent and selective PROTAC degrader for STAT3. RSC Med Chem. 2025 Jul 31.  [Content Brief]