10067 Results for "

Autolysis assay

" in MedChemExpress (MCE) Product Catalog:
Products (10067)

10067 Results for "Autolysis assay" in MCE Product Catalog:

582
Lipopolysaccharides, from E. coli O55:B5
Lipopolysaccharides, from E. coli O55:B5
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582 Publications Verification
製品番号: HY-D1056
別名: LPS, from Escherichia coli (O55:B5)
Category:  

天然物 Microorganisms Disease Research Fields Inflammation/Immunology

Target:  

Toll-like Receptor (TLR)

Lipopolysaccharides, from E. coli O55:B5 (LPS, from Escherichia coli (O55:B5)) are endotoxins and TLR4 activators extracted from Escherichia coli (E. coli O55:B5) and are classified as S (smooth) type LPS. Lipopolysaccharides, from E. coli O55:B5 possess the typical three-part structure: O-antigen, core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activate TLR-4 in immune cells, exhibit high pyrogenicity, and demonstrate dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 can be widely used to induce cellular inflammation and establish animal models related to inflammation .
It is recommended to prepare a solution with concentration ≥2 mg/mL. Vortex thoroughly for more than 10 minutes. Due to the adsorption characteristics of LPS, silanized container or low adsorption centrifuge tubes should be used for aliquoting and storage, and mix thoroughly before use.
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379
Dimethyl sulfoxide, meets analytical specification of Ch.P.
Dimethyl sulfoxide, meets analytical specification of Ch.P.
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379 Cited Publications
製品番号: HY-Y0320
CAS 番号: 67-68-5
別名: DMSO, meets analytical specification of Ch.P.
Dimethyl sulfoxide (DMSO), meets analytical specification of Ch.P. is an aprotic solvent that dissolves polar and non-polar compounds, including water-insoluble therapeutic and toxic agents. Dimethyl sulfoxide (DMSO) has a strong affinity for water and can rapidly penetrate or enhance the penetration of other substances into biological membranes. Dimethyl sulfoxide also has potential free radical scavenging and anticholinesterase effects and may affect coagulation activity. Dimethyl sulfoxide also induces histamine release from mast cells but is thought to have low systemic toxicity. Dimethyl sulfoxide also exhibits antifreeze and antibacterial properties .
MCE provides Dimethyl sulfoxide that complies with the inspection standards (Ch.P) of Part 4 of the Chinese Pharmacopoeia (2020 Edition). Amicrobic, low endotoxin, can be used in various biochemical experiments such as drug dissolution.
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274
Ruxolitinib
Ruxolitinib
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274 Cited Publications
製品番号: HY-50856
CAS 番号: 941678-49-5
別名: INCB18424
Target:  

JAK Autophagy Mitophagy Apoptosis

研究分野:  

Cancer

Ruxolitinib (INCB18424) is an orally active and selective JAK1/2 inhibitor with IC50s of 3.3 nM and 2.8 nM in cell-free assays, and has 130-fold selectivity for JAK1/2 over JAK3 . Ruxolitinib induces autophagy and kills tumor cells through toxic mitophagy .
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212
PEG300
PEG300
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212 Cited Publications
製品番号: HY-Y0873
CAS 番号: 25322-68-3
別名: Polyethylene glycol 300
PEG300 (Polyethylene glycol 300), a neutral polymer of molecular weight 300, is a water-soluble, low immunogenic and biocompatible polymer formed by repeating units of ethylene glycol .
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192
Thiazolyl Blue
Thiazolyl Blue
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192 Cited Publications
製品番号: HY-15924
CAS 番号: 298-93-1
別名: MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .
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173
ATP dimagnesium
ATP dimagnesium
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173 Cited Publications
製品番号: HY-B2176B
CAS 番号: 74804-12-9
別名: Adenosine 5'-triphosphate dimagnesium
Category:  

天然物 Endogenous metabolite Disease Research Fields Inflammation/Immunology

Target:  

Endogenous Metabolite NOD-like Receptor (NLR) Interleukin Related Bacterial

ATP (Adenosine 5'-triphosphate) dimagnesium is a central component of energy storage and metabolism in vivo. ATP dimagnesium provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP dimagnesium is an important endogenous signaling molecule in immunity and inflammation. ATP dimagnesium can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP dimagnesium has anti-bacterial infection effects and can protect mice against bacterial infection in mice .
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168
D-Lin-MC3-DMA
D-Lin-MC3-DMA
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168 Cited Publications
製品番号: HY-112251
CAS 番号: 1224606-06-7
純度:  99.68%
D-Lin-MC3-DMA, an ionizable cationic lipid, is a potent siRNA delivery vehicle.
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166
SBE-β-CD
SBE-β-CD
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166 Cited Publications
製品番号: HY-17031
CAS 番号: 182410-00-0
別名: Sulfobutylether-β-Cyclodextrin
SBE-β-CD is a sulfobutylether β-cyclodextrin derivative used as an excipient or a formulating agent to increase the solubility of poorly soluble agents .
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165
JC-1 Mitochondrial Membrane Potential Assay Kit
JC-1 Mitochondrial Membrane Potential Assay Kit
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165 Cited Publications
製品番号: HY-K0601

MCE JC-1 Mitochondrial Membrane Potential Assay Kit uses JC-1 to detect the mitochondrial membrane potential in variety of cell types, as well as intact tissues and isolated mitochondria.

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164
ATP disodium salt, 100 mM Solution, PCR Grade
ATP disodium salt, 100 mM Solution, PCR Grade
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164 Cited Publications
製品番号: HY-B2176I
CAS 番号: 987-65-5
別名: Adenosine 5'-triphosphate disodium salt, 100 mM Solution, PCR Grade
ATP (Adenosine 5'-triphosphate) disodium salt, 100 mM Solution, PCR Grade is a central component of energy storage and metabolism in vivo. ATP disodium salt, 100 mM Solution, PCR Grade provides the metabolic energy to drive metabolic pumps and serves as a coenzyme in cells. ATP disodium salt, 100 mM Solution, PCR Grade is an important endogenous signaling molecule in immunity and inflammation. ATP disodium salt, 100 mM Solution, PCR Grade can activate the NLRP3 inflammasome and induce IL-1β and chemokines secretion. ATP disodium salt, 100 mM Solution, PCR Grade has anti-bacterial infection effects and can protect mice against bacterial infection in mice .
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158
Corn oil
Corn oil
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158 Cited Publications
製品番号: HY-Y1888
CAS 番号: 8001-30-7
Corn oil, extracted from the germ of corn, can be used as a carrier for agent molecules.
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146
BAPTA-AM
BAPTA-AM
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146 Cited Publications
製品番号: HY-100545
CAS 番号: 126150-97-8
純度:  99.23%
BAPTA-AM is a well-known membrane permeable Ca 2+ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50s of 1.3 μM, 1.45 μM and 1.23 μM, respectively .
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126
D-Luciferin sodium
D-Luciferin sodium
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126 Cited Publications
製品番号: HY-12591
CAS 番号: 103404-75-7
純度:  99.92%
別名: D-(-)-Luciferin sodium; Firefly luciferin sodium; Beetle Luciferin sodium
D-luciferin sodium is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
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126
D-Luciferin
D-Luciferin
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126 Cited Publications
製品番号: HY-12591A
CAS 番号: 2591-17-5
純度:  99.54%
別名: D-(-)-Luciferin; Firefly luciferin; Beetle Luciferin
Category:  

天然物 Animals Other Diseases Disease Research Fields

Target:  

Fluorescent Dye

D-luciferin is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We of er the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
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126
D-Luciferin potassium
D-Luciferin potassium
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126 Cited Publications
製品番号: HY-12591B
CAS 番号: 115144-35-9
純度:  99.97%
別名: D-(-)-Luciferin potassium; Firefly luciferin potassium; Beetle Luciferin potassium
D-luciferin potassium is the natural substrate of the enzyme luciferase (Luc) that catalyzes the production of the typical yellowgreen light of fireflies. The 560 nm chemiluminescence from this reaction peaks within seconds, with light output that is proportional to luciferase concentration when the substrate luciferin is present in excess. The luciferase (luc) gene is a popular reporter gene for research and agent screening. Chemiluminescent techniques are virtually background-free, making the luc reporter gene ideal for detecting low-level gene expression. As little as 0.02 pg of luciferase can be reliably measured in a standard scintillation counter. In addition to its role as a reporter of gene expression, luciferase is commonly used in an extremely sensitive assay for ATP . We offer the firefly luciferase (HY-P1004A), luciferin free acid (HY-12591A), as well as its water-soluble sodium salts (HY-12591) and potassium salts (HY-12591B) .
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126
Tween 80
Tween 80
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126 Cited Publications
製品番号: HY-Y1891
CAS 番号: 9005-65-6
別名: Polysorbate 80
Tween 80 (Polysorbate 80), a surfactant, has been widely used as a solvent for pharmacological experiments. Tween 80 can also reduce bacterial attachment and inhibit biofilm formation.
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116
Palmitic acid sodium
Palmitic acid sodium
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116 Cited Publications
製品番号: HY-N0830B
CAS 番号: 408-35-5
Palmitic acid sodium is a long-chain saturated fatty acid commonly found in both animals and plants. Palmitic acid sodium can induce the expression of glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in in mouse granulosa cells. Palmitic acid sodium is used to establish a cell steatosis model .
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115
Hexadimethrine bromide
Hexadimethrine bromide
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115 Cited Publications
製品番号: HY-112735
CAS 番号: 28728-55-4
純度:  99.69%
別名: Polybrene; 1,5-Dimethyl-1,5-diazaundecamethylene polymethobromide
Hexadimethrine bromide (Polybrene) is a cationic polymer used routinely to enhance the efficiency of retrovirus vector-mediated gene transfer .
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114
Methyl-β-cyclodextrin
Methyl-β-cyclodextrin
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114 Cited Publications
製品番号: HY-101461
CAS 番号: 128446-36-6
純度:  99.95%
別名: Methyl-beta-cyclodextrin
Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a cyclic heptasaccharide used to deliver hydrophobic agents based on its property of solubilizing non-polar substances. Methyl-β-cyclodextrin is also extensively used as a cholesterol-depleting reagent . Methyl-β-cyclodextrin strongly reduces clathrin-dependent endocytosis . Methyl-β-cyclodextrin blocks cell migrasome formation .
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98
Tazemetostat
Tazemetostat
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98 Cited Publications
製品番号: HY-13803
CAS 番号: 1403254-99-8
純度:  99.93%
別名: EPZ-6438; E-7438
Target:  

Histone Methyltransferase Apoptosis

研究分野:  

Cancer

Tazemetostat (EPZ-6438) is a potent, selective and orally available EZH2 inhibitor. Tazemetostat inhibits the activity of human polycomb repressive complex 2 (PRC2)-containing wild-type EZH2 with a Ki value of 2.5 nM. Tazemetostat inhibits EZH2 with IC50s of 11 and 16 nM in peptide assay and nucleosome assay, respectively. Tazemetostat inhibits rat EZH2 with an IC50 of 4 nM. Tazemetostat also inhibits EZH1 with an IC50 of 392 nM. Tazemetostat induces apoptosis and differentiation specifically in SMARCB1-deleted MRT cells .
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