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fluorescence microscopy

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183

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143

Fluorescent Dye

17

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6

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4

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3

Click Chemistry

18

Oligonucleotides

Cat. No. Product Name Target Research Areas Chemical Structure
  • HY-D0718
    Nile Red
    115+ Cited Publications

    Nile Blue A oxazone; Phenoxazone 9

    Fluorescent Dye Others
    Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm .
    Nile Red
  • HY-116215
    2-NBDG
    70+ Cited Publications

    Fluorescent Dye Others
    2-NBDG is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
    2-NBDG
  • HY-D0090
    MQAE
    15+ Cited Publications

    Fluorescent Dye Others
    MQAE is a chloride ion (Cl -) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl - ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm) .
    MQAE
  • HY-111330
    Hydroxyphenyl Fluorescein
    10+ Cited Publications

    HPF; 3'-p-(Hydroxyphenyl) fluorescein

    Fluorescent Dye Reactive Oxygen Species (ROS) Others
    Hydroxyphenyl Fluorescein (HPF) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm .
    Hydroxyphenyl Fluorescein
  • HY-D0988

    R-PE

    Fluorescent Dye Apoptosis Cancer
    R-Phycoerythrin is found in Heterosiphonia japonica. R-Phycoerythrin is an orange-red fluorescent probe with α, β, and γ subunits. R-Phycoerythrin can be used in photodynamic therapy (PDT) to induce apoptosis in tumor cells. R-Phycoerythrin can be used in fluorescence microscopy, flow cytometry, and immunofluorescence analysis (Ex: 495 nm).
    R-Phycoerythrin
  • HY-D0722
    5(6)-CFDA
    5+ Cited Publications

    5-(6)-Carboxyfluorescein diacetate; CFDA

    Fluorescent Dye Others
    5(6)-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes .
    5(6)-CFDA
  • HY-162543

    18:1 Lissamine rhodamine PE

    Fluorescent Dye Others
    18:1 Liss Rhod (18:1 Lissamine rhodamine) PE is a fluorescent phospholipid and fluorescent probe.18:1 Liss Rhod PE admixes into phospholipid inks for large-scale monitoring of dip-pen nanolithography-generated lithographic structures via fluorescence microscopy.18:1 Liss Rhod PE undergoes phase separation or self-quenching under certain conditions in thin lipid membrane stacks .
    18:1 Liss Rhod PE
  • HY-D2001

    Fluorescent Dye Others
    ATTO 488 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 NHS ester is an NHS ester derivative of ATTO 488 that can be used to label proteins or antibodies.
    ATTO 488 NHS ester
  • HY-D0721

    6-Carboxyfluorescein diacetate

    Fluorescent Dye Others
    6-CFDA is a common aliphatic luciferin-line organism. CFDA conducts free diffusion into cells, and then it is hydrolyzed into carboxyl fluorescein (CF) by intracellular non-specific lipase. CF containing portion contains an additional negative charge so that it is better retained in cells, compared to fluorescein dyes .
    6-CFDA
  • HY-DY1008

    Fluorescent Dye Others
    Nile Red (solution) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm .
    Solvent and concentration: DMSO: 1 mM
    Nile Red (solution)
  • HY-D1429

    Fluorescent Dye Others
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206), highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Staining followed by fixation is possible, but cells fixed with aldehydes will only retain partial fluorescence (Ex/Em = 374/ 430-640 nm) .
    ER-Tracker Blue-White DPX
  • HY-DY1019

    Fluorescent Dye Others
    2-NBDG (solution) is a fluorescently-labeled deoxyglucose analog that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence. It has lower photostability than the rhodamine-based fluorescent probes.
    Solvent and Concentration: Sterile PBS: 5 mM
    2-NBDG (solution)
  • HY-NP163A

    WGA-AF488

    Fluorescent Dye Cancer
    Wheat germ agglutinin-AF488 (WGA-AF488) is a cell membrane-specific staining agent prepared by conjugating wheat germ agglutinin with the Alexa Fluor 488 (HY-D1304) fluorescent dye, and it binds to cell surface glycoproteins with high affinity. Wheat germ agglutinin-AF488 is applied in fluorescence microscopy and confocal imaging techniques, and it can clearly label the membrane structures of various cells including breast cancer cells, enabling high-resolution visual observation. Wheat germ agglutinin-AF488 is used in studies of breast cancer and triple-negative breast cancer to observe cell morphology and membrane dynamic changes .
    Wheat germ agglutinin-AF488
  • HY-DY1074

    Fluorescent Dye Others
    ER-Tracker dye is a derivative of BODIPY series dyes coupled with Glibenclamide (HY-15206) , highly selective binding to the endoplasmic reticulum, non-toxic to cells at low concentrations, this type of dye is an environmentally sensitive probe, and formaldehyde treatment can still retain part of the fluorescence, with high fluorescence life, good extinction coefficient and other characteristics. Glibenclamide is an atp-dependent K + channel blocker (Kir6, KATP) and CFTR Cl-channel blocker that binds in the endoplasmic reticulum. ER-Tracker is not suitable for staining fixed cells. Staining followed by fixation is possible, but cells fixed with aldehydes will only retain partial fluorescence .
    Solvent and concentration: DMSO: 1 mM
    ER-Tracker Blue-White DPX (solution)
  • HY-D2001A

    Fluorescent Dye Others
    TTO 488 NHS ester TEA is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. TTO 488 NHS ester TEA is an NHS ester derivative of ATTO 488 that can be used to label proteins or antibodies.
    ATTO 488 NHS ester TEA
  • HY-131009

    PARP Others
    Fluorescein-NAD+ is an alternative to radiolabeled NAD and a substrate for ADP-ribosylation. Fluorescein-NAD+ can be used in PARP assays by fluorescence microscopy. Extinction Coefficient: 262 nm.
    Fluorescein-NAD+
  • HY-D1950

    Fluorescent Dye Others
    ATTO 633 is a fluorescent dye with an absorption peak at approximately 630 nm and a fluorescence emission peak at 651 nm. ATTO 633 can be used in nanomechanical photothermal microscopy studies .
    ATTO 633
  • HY-D1305

    Fluorescent Dye Others
    ATTO 488 carboxylic acid is a new fluorescent label based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 carboxylic acid is a carboxylic acid derivative of ATTO 488, which can be used to label proteins or antibodies.
    ATTO 488 carboxylic acid
  • HY-W440908

    Liposome Others
    DSPE-PEG2000-Cy3 is a PEG lipid conjugated with a fluorophore. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy and flow cytometry. The absorption wavelength of the Cy3 fluorophore peaks at 548-552 nm, while its emission wavelength reaches a maximum at 562-570 nm .
    DSPE-PEG2000-Cy3
  • HY-110334

    Fluorescent Dye Others
    FFN 206 dihydrochloride, a fluorescent probe, is used as an excellent Vesicular Monoamine Transporter 2 (VMAT2) substrate with an apparent Km of 1.16 μM. FFN 206 dihydrochloride is capable of detecting VMAT2 activity in intact cells using fluorescence microscopy, with subcellular localization to VMAT2-expressing acidic compartments without apparent labeling of other organelles .
    FFN 206 dihydrochloride
  • HY-D2438

    Fluorescent Dye Cancer
    CDDP-PEG-Cy3 is a CDDP-PEG conjugate labeled with Cy3 (HY-D0822). The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. The maximum emission wavelength of Cy3 is approximately 562-570 nm. Cisplatin (CDDP) (HY-17394) is an antineoplastic chemotherapy agent by cross-linking with DNA and causing DNA damage in cancer cells. Cisplatin activates ferroptosis and induces autophagy .
    CDDP-PEG-Cy3
  • HY-D2002

    Fluorescent Dye Others
    ATTO 488 maleimide is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 488 maleimide is a maleimide derivative of ATTO 488, which can be used to label proteins or antibodies.
    ATTO 488 maleimide
  • HY-D1959

    Fluorescent Dye Others
    ATTO 565 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 565 NHS ester is an NHS ester derivative of ATTO 565 that can be used to label proteins or antibodies.
    ATTO 565 NHS ester
  • HY-D1240

    Biochemical Assay Reagents Others
    Rhodamine 101 inner salt is a bright fluorescent dye with excitation and emission maxima at 565 and 595 nm, respectively. It can be used in various biological applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy, and ELISA.
    Rhodamine 101 inner salt
  • HY-105552

    Biochemical Assay Reagents Others
    Prolonium (iodide) is membrane impermeant. Prolonium (iodide) can easily penetrate dead or damaged cells. Prolonium (iodide) is a probe that can be used for fluorescence microscopy and flow cytometry .
    Prolonium iodide
  • HY-DN0194F

    Fluorescent Dye Others
    Cy3-Asiatic acid is a fluorescent labeling reagent that combines Cy3 (HY-D0822) and Asiatic acid (HY-N0194). Cy3-Asiatic acid can be used to observe and track Asiatic acid under fluorescence microscopy (Ex/Em = 550/570 nm).
    Cy3-Asiatic acid
  • HY-W440910

    Liposome Others
    DSPE-PEG5000-Cy3 is a phospholipid PEG polymer with Cy3 dye used in labeling and fluorescence microscopy. The polymer can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine.
    DSPE-PEG5000-Cy3
  • HY-DY1035

    Reactive Oxygen Species (ROS) Fluorescent Dye Others
    Hydroxyphenyl Fluorescein (HPF) (solution) is a stable ROS fluorescent probe dye. Hydroxyphenyl Fluorescein has stronger specificity and stability than H2DCFDA (HY-D0940). Hydroxyphenyl Fluorescein can produce strong green fluorescence through hydroxyl radical reaction with intracellular peroxynitroso. Hydroxyphenyl Fluorescein can be applied for fluorescence microscopy, high-throughput imager, luciferase microplate reader or flow cytometry. Ex/Em=490/515 nm .
    Solvent and concentration: DMSO: 10 mM
    Hydroxyphenyl Fluorescein (solution)
  • HY-152901

    Fluorescent Dye Others
    Chol-N3 is a bioorthogonal-based chol probe. Chol-N3 can combine with super-resolution fluorescence microscopy (SRM), providing direct visualization of nanoscale lipid heterogeneity in the cell surface of resting living cells . Chol-N3 is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
    Chol-N3
  • HY-153524

    Fluorescent Dye Others
    ATTO 425 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 425 NHS ester is an NHS ester derivative of ATTO 425 that can be used to label proteins or antibodies.
    ATTO 425 NHS ester
  • HY-D1993

    Fluorescent Dye HIV Infection
    ATTO 647 NHS ester is a fluorescent label targeting free amino groups. ATTO 647 NHS ester can undergo a nucleophilic reaction with the free amino groups of EF-C peptide via its activated carboxylic acid group to form a stable covalent conjugate. ATTO 647 NHS ester-labeled nanofibers not only retain retroviral transduction-enhancing activity but also maintain stable fluorescent signals in both buffer and cell culture systems. ATTO 647 NHS ester is suitable for detection applications including fluorescence spectroscopy, microscopy and flow cytometry. ATTO 647 NHS ester has been used in studies related to HIV-1 infection .
    ATTO 647 NHS ester
  • HY-N16309

    Fluorescent Dye Others
    Cyanine 7 free acid bromide is a near-infrared fluorescent probe for labelling amine groups such as those on antibodies, nucleic acids, and proteins and can be detected using a variety of fluorescence detection techniques such as microscopy and flow cytometry.
    Cyanine 7 free acid bromide
  • HY-D2506

    Fluorescent Dye Others
    Cy3-PEG3400-NH2 is a polyethylene glycol derivative containing Cy3 (HY-D0822) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. Cy3-PEG3400-NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling .
    Cy3-PEG3400-NH2
  • HY-W440912

    Liposome Others
    DSPE-PEG3400-Cy5 is a PEG phospholipid with Cy5 dye used in protein/nucelic acid labeling and fluorescence microscopy. The polymer can self-assemble in aqueous solution to form micelles/lipid bilayer and used to prepare liposomes or nanoparticles for nutrients delivery such as mRNA or DNA vaccine.
    DSPE-PEG3400-Cy5
  • HY-D2440

    Fluorescent Dye Others
    cRGD-PEG-Cy3 is a Cy3 (HY-D0822) labeled cRGD-PEG conjugate. The Cy3 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy3 has an emission maximum around 562-570 nm. cRGD can be used to modify lipid for improved stability .
    cRGD-PEG-Cy3
  • HY-D1915

    Fluorescent Dye Others
    ATTO 390 is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy.
    ATTO 390
  • HY-D2046

    Fluorescent Dye Others
    ATTO 532 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 532 NHS ester is an NHS ester derivative of ATTO 532 that can be used to label proteins or antibodies.
    ATTO 532 NHS ester
  • HY-D1957

    Fluorescent Dye Others
    ATTO 633 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 633 NHS ester is an NHS ester derivative of ATTO 633 that can be used to label proteins or antibodies.
    ATTO 633 NHS ester
  • HY-D2019

    Fluorescent Dye Others
    ATTO 550 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 550 NHS ester is an NHS ester derivative of ATTO 550 that can be used to label proteins or antibodies.
    ATTO 550 NHS ester
  • HY-D2016

    Fluorescent Dye Others
    ATTO 565 cadaverine is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 565 cadaverine is a cadaverine derivative of ATTO 565, which can be used to label proteins or antibodies.
    ATTO 565 cadaverine
  • HY-D2058

    Fluorescent Dye Others
    ATTO 700 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 700 NHS ester is an NHS ester derivative of ATTO 700 that can be used to label proteins or antibodies.
    ATTO 700 NHS ester
  • HY-D2073

    Fluorescent Dye Others
    ATTO 680 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 680 NHS ester is an NHS ester derivative of ATTO 680 that can be used to label proteins or antibodies.
    ATTO 680 NHS ester
  • HY-D2021

    Fluorescent Dye Others
    ATTO 550 maleimide is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 550 maleimide is a maleimide derivative of ATTO 550, which can be used to label proteins or antibodies.
    ATTO 550 maleimide
  • HY-D1935

    Fluorescent Dye Others
    ATTO 465 NHS ester is a new fluorescent marker based on the Rhodamine structure. It has strong absorption, high fluorescence quantum yield, high thermal stability and photochemical stability, and is suitable for single molecule detection and high-resolution microscopy. ATTO 465 NHS ester is an NHS ester derivative of ATTO 465 that can be used to label proteins or antibodies.
    ATTO 465 NHS ester
  • HY-131009A

    PARP Others
    Fluorescein-NAD+ sodium is an alternative to radiolabeled NAD and a substrate for ADP-ribosylation. Fluorescein-NAD+ sodium can be used in PARP assays by fluorescence microscopy. Extinction Coefficient: 262 nm.
    Fluorescein-NAD+ sodium
  • HY-D2602

    Fluorescent Dye Others
    ICG PEG5000 NH2 is a polyethylene glycol derivative containing Indocyanine green (ICG) (HY-D0711) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Indocyanine green fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. ICG PEG5000 NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling (Ex/Em=785/813 nm).
    ICG PEG5000 NH2
  • HY-D2599

    Fluorescent Dye Others
    ICG PEG1000 NH2 is a polyethylene glycol derivative containing Indocyanine green (ICG) (HY-D0711) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Indocyanine green fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. ICG PEG1000 NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling (Ex/Em=785/813 nm).
    ICG PEG1000 NH2
  • HY-D2554

    Fluorescent Dye Others
    Cy5.5-PEG5000-NH2 is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy5.5 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy5.5 has an emission maximum around 710 nm. Cy5.5-PEG5000-NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling.
    Cy5.5-PEG5000-NH2
  • HY-D2553

    Fluorescent Dye Others
    Cy5.5-PEG3400-NH2 is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy5.5 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy5.5 has an emission maximum around 710 nm. Cy5.5-PEG3400-NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling.
    Cy5.5-PEG3400-NH2
  • HY-D2552

    Fluorescent Dye Others
    Cy5.5-PEG1000-NH2 is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and an amino group. The Cy5.5 fluorophore is commonly used in applications such as immunolabeling, nucleic acid labeling, fluorescence microscopy, and flow cytometry. Cy5.5 has an emission maximum around 710 nm. Cy5.5-PEG1000-NH2 can be used for fluorescence imaging, fluorescence tracing and fluorescence labeling.
    Cy5.5-PEG1000-NH2

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