494-38-2
494-38-2
Chemical Structure
Acridine Orange base
- CAS No.: 494-38-2
- Formula:C17H19N3
- Molecular Weight:265.36
IUPAC Name: N3,N3,N6,N6-tetramethylacridine-3,6-diamine
InChIKey: DPKHZNPWBDQZCN-UHFFFAOYSA-N
SMILES: CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1
Biological Activity: Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Acridine Orange base | 97.04% | Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays. | ||||||||||||||||||||
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Acridine Orange hydrochloride (Standard) | ≥98% | Acridine Orange hydrochloride (Standard) is the analytical standard of Acridine Orange hydrochloride (HY-101879). This product is intended for research and analytical applications. Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm). | ||||||||||||||||||||
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Arylquin 1 | 99.7% | Arylquin 1, a prostate-apoptosis-response-4 (Par-4) secretagogue, targets vimentin to induce Par-4 secretion. Arylquin 1 induces non-apoptotic cell death in cancer cells through the induction of lysosomal membrane permeabilization (LMP). | ||||||||||||||||||||
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Methylene blue (purity≥70%) | 95.0% | Methylene blue (purity≥70%) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue (purity≥70%) is a vasopressor and is often used as a dye in several medical procedures. Methylene blue (purity≥70%) through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue (purity≥70%) is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue (purity≥70%) is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation. | ||||||||||||||||||||
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3,6-Diaminoacridine | 98.58% | 3,6-Diaminoacridine is a versatile acridine compound. 3,6-Diaminoacridine exerts antibacterial activity by intercalating into bacterial DNA, interfering with replication and transcription, and inducing bacterial lysis. 3,6-Diaminoacridine is an acridine dye and also a DNA intercalator. | ||||||||||||||||||||
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3,6-Diaminoacridine dihydrochloride | 3,6-Diaminoacridine dihydrochloride is a versatile acridine compound. 3,6-Diaminoacridine dihydrochloride exerts antibacterial activity by intercalating into bacterial DNA, interfering with replication and transcription, and inducing bacterial lysis. 3,6-Diaminoacridine dihydrochloride is an acridine dye and also a DNA intercalator. | |||||||||||||||||||||
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Acridine Orange zinc chloride salt | Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm). | |||||||||||||||||||||
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Pyronin Y | 83.71% | Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. | ||||||||||||||||||||
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Selenomethylene blue | Selenomethylene blue is an antioxidant and anti-inflammatory agent. Selenomethylene blue effectively inhibits the inflammatory paw edema in rats. | |||||||||||||||||||||
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3,6-Diaminoacridine hydrochloride, 98% | 3,6-Diaminoacridine hydrochloride, 98% is used as a preservative and antibacterial agent. | |||||||||||||||||||||
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3,6-Diaminoacridine sulfate | 99.29% | Proflavine sulfate is a multifunctional acridine compound. 3,6-Diaminoacridine sulfate is an acridine dye and also a DNA inserter. Proflavine sulfate is a potent broad-spectrum antibacterial agent, and its mechanism is to insert into bacterial DNA, interfering with replication and transcription, causing bacterial lysis. 3,6-Diaminoacridine sulfate is a Kir3.2 potassium channel blocker and can be used to study the neurological phenotype of Down syndrome. 3,6-Diaminoacridine sulfate can penetrate the stratum corneum of the skin and accumulate in the cell nucleus and long-term exposure may induce skin cancer or other malignant tumors. | ||||||||||||||||||||
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References
- [1]. Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3. [Content Brief]
- [2]. Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3(3):250-2. [Content Brief]
- [3]. Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5(12):e03073. [Content Brief]
