494-38-2
Chemical Structure
Acridine Orange base
- CAS No.: 494-38-2
- Formula:C17H19N3
- Molecular Weight:265.36
IUPAC Name: N3,N3,N6,N6-tetramethylacridine-3,6-diamine
InChIKey: DPKHZNPWBDQZCN-UHFFFAOYSA-N
SMILES: CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1
Biological Activity: Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].
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Acridine Orange base | 96.58% | Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays. | ||||||||||||||||||||
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- [1]. Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov;Chapter 7:Unit 7.3. [Content Brief]
- [2]. Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3(3):250-2. [Content Brief]
- [3]. Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5(12):e03073. [Content Brief]
Keywords
