2. Cell Cycle/DNA Damage
  3. DNA Stain
  4. Pyronin Y

Pyronin Y  (Synonyms: Pyronine G; C.I. 45005)

Cat. No.: HY-D0971 Purity: 78.32%
COA Handling Instructions

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Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells.

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Pyronin Y Chemical Structure

Pyronin Y Chemical Structure

CAS No. : 92-32-0

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Based on 3 publication(s) in Google Scholar

Pyronin Y Related Antibodies

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Description

Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells[1][2][5].

In Vitro

Pyronin Y forms fluorescent complexes with double-stranded nucleic acids, especially RNA, enabling semi-quantitative analysis of cellular RNA in flow cytometry, to estimate the RNA content per cell in formalin fixed EL4 leukosis tumor cells, enzyme dispersed R3327-G rat prostatic adenocarcinoma cells, mouse spleen cells stimulated with concanavalin A, and human peripheral blood lymphocytes stimulated with phytohemagglutinin[1].
A fluorescent staining procedure based on pyronin Y is described. The technique has been used to stain RNA in human reticulocytes for subsequent flow analysis and sorting[2].
In viable cells this dye also accumulates in mitochondria. At a concentration of 1.7 to 3.3 μM, pyronin Y is localized almost exclusively in mitochondria of cultured cells, similar to another mitochondria! probe, rhodamine 123. At that concentration PY is not toxic but suppressed cell growth, arresting cells[3].
Pyronin Y has long been used, in combination with other dyes such as Methyl Green, as a differential stain for nucleic acids in paraffin tissue sections. In sections stained with Methyl Green-pyronin Y, red blood cells, elastic fibre of blood vessels, zymogen granules of pancreatic acinar cells, surface membrane of heptocytes and kidney tubular cells showed strikingly strong green and/or red fluorescence, while the nuclei of cells appeared non-fluorescent[4].
Pyronin Y binds to double stranded RNA (dsRNA) including messenger RNA (mRNA), transfer RNA (tRNA) and ribosomal RNA (rRNA)[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyronin Y Related Antibodies
Molecular Weight

302.80

Formula

C17H19ClN2O
CAS No.
Appearance

Solid

Color

Light brown to black

SMILES

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C=C2C=C1)C.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (82.56 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 4 mg/mL (13.21 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3025 mL 16.5126 mL 33.0251 mL
5 mM 0.6605 mL 3.3025 mL 6.6050 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

g

Dosing volume
(per animal)

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 78.32%

COA (246 KB) HNMR (260 KB) LCMS (113 KB)

Handling Instructions (2659 KB)
Dyeing Example
References
Cell Assay
[2]

The cells are resuspended and stained for 30 min in 1.0 mL of 0.01% pyronin Y in sodiumacetate buffer (1.0 M, pH 4.7). For this purpose 1.0 g of pyronin Y is dissolved in 100 mL distilled water and this solution is purified by chloroform extraction (three fractions of 100 mL). The purified pyronin Y solution is diluted with 1.0 M sodiumacetate buffer pH 4.7 to the appropriate concentration and filtered through paper filters before use. Stained cell samples are studied by fluorescence microscopy (excitation filters SP 560 and LP 515, chromatic beam splitter at 580 nm, barrier filter LP 580) or by flow cytometry[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.3025 mL 16.5126 mL 33.0251 mL 82.5628 mL
5 mM 0.6605 mL 3.3025 mL 6.6050 mL 16.5126 mL
10 mM 0.3303 mL 1.6513 mL 3.3025 mL 8.2563 mL
DMSO 15 mM 0.2202 mL 1.1008 mL 2.2017 mL 5.5042 mL
20 mM 0.1651 mL 0.8256 mL 1.6513 mL 4.1281 mL
25 mM 0.1321 mL 0.6605 mL 1.3210 mL 3.3025 mL
30 mM 0.1101 mL 0.5504 mL 1.1008 mL 2.7521 mL
40 mM 0.0826 mL 0.4128 mL 0.8256 mL 2.0641 mL
50 mM 0.0661 mL 0.3303 mL 0.6605 mL 1.6513 mL
60 mM 0.0550 mL 0.2752 mL 0.5504 mL 1.3760 mL
80 mM 0.0413 mL 0.2064 mL 0.4128 mL 1.0320 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Pyronin Y Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyronin Y92-32-0Pyronine G C.I. 45005DNA StaindsRNAmRNAtRNArRNAInhibitorinhibitorinhibit

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