92-32-0
92-32-0
Chemical Structure
Pyronin Y
Synonym(s): Pyronine G; C.I. 45005
- CAS No.: 92-32-0
- Formula:C17H19ClN2O
- Molecular Weight:302.80
IUPAC Name: 3,6-bis(dimethylamino)xanthylium chloride
InChIKey: INCIMLINXXICKS-UHFFFAOYSA-M
SMILES: CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C=C2C=C1)C.[Cl-]
Biological Activity: Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells[1][2][5].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Pyronin Y | 83.71% | Pyronin Y (Pyronine G) is a cationic dye that intercalates RNA and has been used to target cell structures including RNA, DNA and organelles. Pyronin Y forms fluorescent complexes with double-stranded nucleic acids (especially RNA) enabling semi-quantitative analysis of cellular RNA. Pyronin Y can be used to identify specific RNA subspecies of ribonuclear proteins complexes in live cells. | ||||||||||||||||||||
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Acridine Orange hydrochloride | 99.65% | Acridine Orange hydrochloride is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange hydrochloride produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm). | ||||||||||||||||||||
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KMS88009 | KMS88009 is a potent small molecule that directly interferes with the formation of amyloid-β oligomers, thereby preserving cognitive behavior when used preventively and reversing cognitive behavior decline when used therapeutically. Oral administration of KMS88009 around the onset of Alzheimer's disease symptoms significantly reduced the assembly of amyloid-β oligomers and improved cognitive behavior in the APP/PS1 double transgenic mouse model. This unique dual mode of action suggests that KMS88009 may be a powerful therapeutic candidate for the treatment of Alzheimer's disease. In an evaluation, the physicochemical properties, pharmacokinetics and toxicity of this anti-amyloidogenic small molecule KMS88009 were studied, as well as post-mortem analysis of APP/PS1 TG mice after behavioral testing. | |||||||||||||||||||||
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Prodan | 99.71% | Prodan is an environment-sensitive fluorescent probe used to investigate the polarity, fluidity, and structure of lipid membranes. Prodan exhibits environment-dependent excitation/emission wavelengths, with its wavelength range covering Ex = 360-410 nm and Em = 430-530 nm when in solvents, bound to DNA, located in lipid bilayers, or associated with proteins. Prodan can interact with the major groove of DNA, regions of lipid bilayers, and hydrophobic pockets of proteins. During phase transitions, Prodan localizes to different regions of the bilayer: the emission minimum is 430-440 nm in the gel phase, while it shifts to 480-500 nm in the liquid-crystalline phase or interdigitated gel phase. When bound to rigid hydrophobic sites of proteins, the emission peak of Prodan undergoes a blue shift and its fluorescence intensity increases. | ||||||||||||||||||||
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Auramine O | 83.12% | Auramine O is a yellow fluorescent dye. Auramine O is used to detect amyloid fibrils. Auramine O promotes lung malignancy. Auramine O is also used to determine algal cell viability and stain acid-fast bacteria. | ||||||||||||||||||||
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Acridine Orange zinc chloride salt | Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm). | |||||||||||||||||||||
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Acridine Orange base | 97.04% | Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays. | ||||||||||||||||||||
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BADAN | BADAN is a thiol-reactive, environment-sensitive fluorescent probe used for site-specific labeling of protein cysteine residues to detect protein topology, local polarity, protein-lipid interactions, and conformational changes. The bromoacetyl group of BADAN covalently binds to cysteine thiols, and its fluorescence originates from the intramolecular charge transfer (ICT) excited state. The emission peak position varies with the polarity of the surrounding medium: it blue-shifts to approximately 488 nm in nonpolar environments (such as the hydrophobic core of lipid bilayers) and red-shifts to approximately 496-507 nm in polar environments (such as aqueous phases), independent of enzymes, pH, or membrane potential. Ex = 380-402 nm; Em = 400-600 nm. | |||||||||||||||||||||
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Pyronin B | Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor. | |||||||||||||||||||||
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Thiomichler's ketone | 81.08% | Thiomichler's ketone (4,4'-Bis (dimethylamino) thiobenzophenone) is a heavy metal complexing agent and a colorimetric/spectrophotometric reagent. Thiomichler's ketone shows selectivity for Hg2+ at pH 3, and exhibits activity towards Pd2+ and Ag+ at pH 3.5. Thiomichler's ketone enables accurate detection of trace Pd2+ by forming an extractable Hg2+ complex via micelle-mediated cloud point extraction, or generating a red coordination complex including Pd (TMK)4. Thiomichler's ketone is applicable for the determination of trace Pd2+ in antibiotics and catalysts of automobile exhaust purifiers. | ||||||||||||||||||||
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Acrylodan | 95.05% | Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34. | ||||||||||||||||||||
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References
- [1]. Pollack A, et al. Flow cytometric analysis of RNA content in different cell populations using pyronin Yand methyl green. Cytometry. 1982 Jul;3(1):28-35. [Content Brief]
- [2]. Tanke HJ, et al. Flow cytometry of human reticulocytes based on RNA fluorescence. Cytometry. 1981 Mar;1(5):313-20. [Content Brief]
- [5]. Laura M Andrews, et al. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed Opt Express. 2013 Jan 1;4(1):171-7. [Content Brief]
