1. Auramine O

Auramine O is a yellow fluorescent dye and can be used to stain acid-fast bacteria. Auramine O is toxic and resistant in the environment.

For research use only. We do not sell to patients.

Auramine O Chemical Structure

Auramine O Chemical Structure

CAS No. : 2465-27-2

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Description

Auramine O is a yellow fluorescent dye and can be used to stain acid-fast bacteria. Auramine O is toxic and resistant in the environment[1][2].

Molecular Weight

303.83

Formula

C17H22ClN3

CAS No.
Appearance

Solid

Color

Light yellow to yellow

SMILES

N=C(C1=CC=C(N(C)C)C=C1)C2=CC=C(N(C)C)C=C2.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 16.67 mg/mL (54.87 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2913 mL 16.4566 mL 32.9131 mL
5 mM 0.6583 mL 3.2913 mL 6.5826 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: ≥95.0%

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2913 mL 16.4566 mL 32.9131 mL 82.2829 mL
5 mM 0.6583 mL 3.2913 mL 6.5826 mL 16.4566 mL
10 mM 0.3291 mL 1.6457 mL 3.2913 mL 8.2283 mL
15 mM 0.2194 mL 1.0971 mL 2.1942 mL 5.4855 mL
20 mM 0.1646 mL 0.8228 mL 1.6457 mL 4.1141 mL
25 mM 0.1317 mL 0.6583 mL 1.3165 mL 3.2913 mL
30 mM 0.1097 mL 0.5486 mL 1.0971 mL 2.7428 mL
40 mM 0.0823 mL 0.4114 mL 0.8228 mL 2.0571 mL
50 mM 0.0658 mL 0.3291 mL 0.6583 mL 1.6457 mL
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Product Name:
Auramine O
Cat. No.:
HY-D0933
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