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  3. Hydroxyphenyl Fluorescein

Hydroxyphenyl Fluorescein (Synonyms: HPF)

Cat. No.: HY-111330
Handling Instructions

Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence.

For research use only. We do not sell to patients.

Hydroxyphenyl Fluorescein Chemical Structure

Hydroxyphenyl Fluorescein Chemical Structure

CAS No. : 359010-69-8

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Description

Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence[1][2].

In Vitro

The fluorescence of Hydroxyphenyl Fluorescein (HPF) is greatly enhanced following incubation with blue light illuminated blebbistatin (30 min). Fluorescence enhancement of Hydroxyphenyl Fluorescein occurred equally efficiently under Ar purged and non-Ar purged conditions, suggesting that singlet oxygen as an intermediate involved is improbable. Fluorescence measurement with Hydroxyphenyl Fluorescein suggests a negative linear relationship between fluorescence intensity of Hydroxyphenyl Fluorescein (10 μM) and thiourea concentration (0-1 mM)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The Hydroxyphenyl Fluorescein (HPF) probe (15 μl, 25 μM) is given intratesticularly in anaesthetized mice 20 min before IR (ionizing radiation). The accumulation of •OH by the fluorescence signal emitted by the oxidized form of HPF is assessed[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

424.40

Formula

C26H16O6

CAS No.
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Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Hydroxyphenyl Fluorescein
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