1. Dye Reagents

Dihydrorhodamine 123 (Synonyms: DHR 123)

Cat. No.: HY-101894
Handling Instructions

Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm).

For research use only. We do not sell to patients.

Dihydrorhodamine 123 Chemical Structure

Dihydrorhodamine 123 Chemical Structure

CAS No. : 109244-58-8

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5 mg USD 228 Get quote

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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Dihydrorhodamine 123 is a fluorescent probe (λex=488 nm, λem=525 nm).

In Vitro

In the presence of 10 μM Dihydrorhodamine 123 (DHR) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8870 mL 14.4350 mL 28.8700 mL
5 mM 0.5774 mL 2.8870 mL 5.7740 mL
10 mM 0.2887 mL 1.4435 mL 2.8870 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[1]

The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2- is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

346.38

Formula

C₂₁H₁₈N₂O₃

CAS No.

109244-58-8

SMILES

O=C(OC)C1=CC=CC=C1C2C3=C(OC4=C2C=CC(N)=C4)C=C(N)C=C3

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Dihydrorhodamine 123
Cat. No.:
HY-101894
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Dihydrorhodamine 123

Cat. No.: HY-101894