2. Others
  3. Fluorescent Dye
  4. Dihydrorhodamine 123

Dihydrorhodamine 123  (Synonyms: DHR 123)

Cat. No.: HY-101894 Purity: 99.83%
COA
SDS
Handling Instructions Technical Support

Dihydrorhodamine 123 (DHR 123) est une sonde fluorescente (λex=488 nm, λem=525 nm).

Dihydrorhodamine 123 (DHR 123) is a non-fluorescent reactive oxygen species (ROS) indicator. Dihydrorhodamine 123 is oxidized to fluorescent Rhodamine 123 (HY-D0816) within cells in the presence of reactive oxygen species and it localizes in mitochondria (Ex/Em = 515/536 nm).

For research use only. We do not sell to patients.

CAS No. : 109244-58-8

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 20 publication(s) in Google Scholar

Other Forms of Dihydrorhodamine 123:

Dihydrorhodamine 123 Related Antibodies

Top Publications Citing Use of Products

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2025 Jan 21;19(2):2822-2833.  [Abstract]

    O2−·detection via DHR 123 (10 μM) in water under irradiation (660 nm, 20 mW/cm2) at an interval of 20 s.

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: Food Packag Shelf Life. 2025 Sep.

    The curve of fluorescence emission intensity of BA and BBR at 1.34 × 10− 4 mol/L with the change of solvent composition ⋅O2- generation ofBA (1.34 ×10− 4 mol/L) and BBR (1.34 ×10− 4 mol/L) in different solutions under white light irradiation (60 mW/cm2) detected by DHR123 (10 μM; 5 min).

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2024 Sep 3:e2402079.  [Abstract]

    Fluorescence images of MCF-7 cells treated with different-sized Fe3O4, SiO2, and Au NPs and stained with Dihydrorhodamine 123 to characterize the generated H2O2. Green fluorescence: Dihydrorhodamine 123 (DHR123, 10 μM, 30 min), an indicator of H2O2; blue fluorescence: Hoechst 33342.

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2023 May 1:200:73-86.  [Abstract]

    The Dihydroethidium (DHE; 10 μM; 1 h）, Dihydrorhodamine 123 (DHR 123, 5 μM, 1 h), FerroOrange and C11 BODIPY probes detect O2• − (red fluorescence),OONO − (green fluorescence), Fe 2+ (orange fluorescence), and lipid peroxides (red fluorescence in the unoxidized state and green fluorescence in the oxidized state), respectively, In normal lung tissue from 10 smoking patients and 10 non-smoking patients.

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2023 May 1:200:73-86.  [Abstract]

    The Dihydroethidium (DHE; 5-20 μM; 1 h）, Dihydrorhodamine 123 (DHR 123, 5 μM, 15-60 min) probes were used to detect ROS intensities in CS-induced bronchial epithelial ferroptosis. Control, and 0.5%,1%, and 2% CSE were used to stimulate BEAS-2B cells for 48 h.

    Dihydrorhodamine 123 purchased from MedChemExpress. Usage Cited in: Commun Biol. 2023 Mar 11;6(1):259.  [Abstract]

    Dihydrorhodamine 123 detectes the mitochondrial ROS level in mouse tumors. Left panels: Representative histograms. Right panels: Quantification of the MFI values.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Dihydrorhodamine 123 (DHR 123) is a non-fluorescent reactive oxygen species (ROS) indicator. Dihydrorhodamine 123 is oxidized to fluorescent Rhodamine 123 (HY-D0816) within cells in the presence of reactive oxygen species and it localizes in mitochondria (Ex/Em = 515/536 nm).

    In Vitro

    Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
    1. Solution preparation
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 10 mM.
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 1-10 μM with cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 Treat cells with inducers or inhibitors of reactive oxygen species. Note: If treating cells with oxidizing agents, rinse cells three times with buffer or culture medium to remove the compounds before staining to avoid direct oxidation of the dye by the compound.
    2.2 Dilute Dihydrorhodamine 123 dye stock solution in serum-free culture medium at a final concentration of 5 μM.
    2.3 Remove medium from the cells and replace with medium containing dye.
    2.4 Incubate for 30 minutes at 37°C.
    2.5 Detect by fluorescence microscopy or flow cytometry. Washing is not required, but can be performed to reduce background for microscopy.

    In the presence of 10 μM Dihydrorhodamine 123 (DHR 123) the stimulation of the neutrophil NADPH oxidase by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) resultes in an increase in the rate of rhodamine generation. The fluorescent intensity of the cells, in the presence of 10 μM Dihydrorhodamine 123, increases with time following the addition of 50 nM PMA. In the presence of 10 μM Dihydrorhodamine 123, induced HL60 cells show a sustained increase in fluorescence following the addition of 50 nM PMA[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Dihydrorhodamine 123 Related Antibodies
    Molecular Weight

    346.38

    Formula

    C21H18N2O3
    CAS No.
    Appearance

    Solid

    Color

    White to pink

    Emission (Em)

    536
    Excitation (Ex)

    515
    SMILES

    O=C(OC)C1=CC=CC=C1C2C3=C(OC4=C2C=CC(N)=C4)C=C(N)C=C3
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *The compound is unstable in solutions, freshly prepared is recommended.

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (288.70 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8870 mL 14.4350 mL 28.8700 mL
    5 mM 0.5774 mL 2.8870 mL 5.7740 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.83%

    SDS (396 KB)

    COA (251 KB) HNMR (260 KB) LCMS (270 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Cell Assay
    [1]

    The HL60 cells are incubated at 6×106 cells/mL in Krebs-Ringer buffer at 37°C containing 10 μM Dihydrorhodamine 123 (DHR). The generation of O2- is initiated by the addition of 50 nM phorbol 12-myristate 13-acetat (PMA) and the progress of the generation of rhodamine 123 is monitored in 50-μL aliquots (3×105 cells) diluted tenfold before analysis. The uninduced HL60 cells are loaded with 5 μM carboxy SNARF-1 AM acetate (SNARF-AM) in the Na+ medium for 10 min at 37°C and washed by centrifugation and resuspension to remove unhydrolysed SNARF ester[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. The compound is unstable in solutions, freshly prepared is recommended.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8870 mL 14.4350 mL 28.8700 mL 72.1751 mL
    5 mM 0.5774 mL 2.8870 mL 5.7740 mL 14.4350 mL
    10 mM 0.2887 mL 1.4435 mL 2.8870 mL 7.2175 mL
    15 mM 0.1925 mL 0.9623 mL 1.9247 mL 4.8117 mL
    20 mM 0.1444 mL 0.7218 mL 1.4435 mL 3.6088 mL
    25 mM 0.1155 mL 0.5774 mL 1.1548 mL 2.8870 mL
    30 mM 0.0962 mL 0.4812 mL 0.9623 mL 2.4058 mL
    40 mM 0.0722 mL 0.3609 mL 0.7218 mL 1.8044 mL
    50 mM 0.0577 mL 0.2887 mL 0.5774 mL 1.4435 mL
    60 mM 0.0481 mL 0.2406 mL 0.4812 mL 1.2029 mL
    80 mM 0.0361 mL 0.1804 mL 0.3609 mL 0.9022 mL
    100 mM 0.0289 mL 0.1444 mL 0.2887 mL 0.7218 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Dihydrorhodamine 123 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Dihydrorhodamine 123109244-58-8DHR 123Dihydrorhodamine123Dihydrorhodamine-123DHR123DHR-123Fluorescent DyeInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    Dihydrorhodamine 123
    Cat. No.:
    HY-101894
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (396 KB) Français - FR (396 KB) Deutsch - DE (396 KB) Norwegian - NO (396 KB) Español - ES (396 KB) Swedish - SV (396 KB) Italian - IT (396 KB) Korean - KR (396 KB) Portuguese - PT (396 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.