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  4. Sennoside A

Sennoside A is an anthraquinone glycoside found in senna (Cassia angustifolia). Sennoside A is an HIV-1 inhibitor (IC50=3.8 μM) that inhibits HIV-1 replication. Sennoside A also inhibits HIV-1 reverse transcriptase (RT)-related DNA polymerase (RDDP) and ribonuclease H (Ribonuclease H) with IC50s of 1.9 μM and 5.3 μM, respectively.

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CAS No. : 81-27-6

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Customer Review

Based on 6 publication(s) in Google Scholar

Other Forms of Sennoside A:

Sennoside A Related Antibodies

Top Publications Citing Use of Products

    Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464.  [Abstract]

    The cell viability was detected by CCK-8 assays after HOK cells were treated with 0, 6.25, 12.5, 25, 50, 100, 200 and 400 μM of Sennoside A (SA) for 48 hours.

    Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464.  [Abstract]

    The relative protein expression of GPX4 and xCT was examined by western blot after SCC7 and CAL27 cells were treated with 25, 50 and 100 μM Sennoside A (SA) for 48 hours.

    Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464.  [Abstract]

    The relative lipid ROS level was examined after SCC7 and CAL27 cells were incubated with 25, 50 and 100 μM Sennoside A (SA) for 48 hours.

    Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464.  [Abstract]

    Measurement of IFN-γ, IL-2 and TNF-α by ELISA incubated with 25, 50 and 100 μM Sennoside A (SA).

    Sennoside A purchased from MedChemExpress. Usage Cited in: Integr Cancer Ther. 2025 Jan-Dec:24:15347354251375464.  [Abstract]

    Pathological examination by HE staining, and the expression levels of GPX4, PD-L1, and IFN-γ in tumor tissues were detected by immunohistochemistry treated with Sennoside A (SA) (10 mg/kg, i.p.).

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    • References

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    Description

    Sennoside A is an anthraquinone glycoside found in senna (Cassia angustifolia). Sennoside A is an HIV-1 inhibitor (IC50=3.8 μM) that inhibits HIV-1 replication. Sennoside A also inhibits HIV-1 reverse transcriptase (RT)-related DNA polymerase (RDDP) and ribonuclease H (Ribonuclease H) with IC50s of 1.9 μM and 5.3 μM, respectively[1][2][3][4].

    IC50 & Target

    HIV-1

     

    DNA Polymerase

     

    Cellular Effect
    Cell Line Type Value Description References
    HCT-116 IC50
    68.2 μM
    Compound: 7
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp under-expressing human HCT116 cells after 24 hrs by MTT assay
    		[PMID: 17949858]
    HepG2 IC50
    51.6 μM
    Compound: 7
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    Cytotoxicity against MDR1 Pgp overexpressing human HepG2 cells after 24 hrs by MTT assay
    		[PMID: 17949858]
    In Vitro

    Sennoside A inhibits different variants of RDDP and RNase H. Inhibits different variants of RDDP with IC50s of 78 μM (K103N RT), 21.3 μM (Y181C RT), and 64 μM (Y188L RT), respectively. Inhibits different variants of RNase H with IC50s of 18.4 μM (N474A RT) and 17.7 μM (Q475A RT), respectively[3].
    Infects Jurka cells with HIV-1 recombinant CAT virus, which is pseudotyped with the envelope glycoprotein from the HXBc2 laboratory-adapted T-tropic virus. Sennoside A (5-20 μM; 72 h) significantly inhibits CAT activity in infected cell[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Sennoside A Related Antibodies
    In Vivo

    Sennoside A (25 mg/kg, 50 mg/kg; intragastric gavage for 12 weeks) alters the gut microbiome composition of type 2 diabetes (T2D) mice and mediates anti-obesity effects[3].
    Sennoside A also reduces inflammation and increases tight junction proteins in the ileum of genetically defective mice[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    862.74

    Formula

    C42H38O20
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C(C=C1[C@@]([C@]2([H])C3=C(C(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO)O4)O)O)O)=CC=C3)C(C5=C(O)C=C(C(O)=O)C=C25)=O)([H])C6=C7C(O[C@H]8[C@@H]([C@H]([C@@H]([C@@H](CO)O8)O)O)O)=CC=C6)=CC(O)=C1C7=O)O
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (115.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.1591 mL 5.7955 mL 11.5910 mL
    5 mM 0.2318 mL 1.1591 mL 2.3182 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 6.25 mg/mL (7.24 mM); Suspended solution; Need ultrasonic

      This protocol yields a suspended solution of 6.25 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (62.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.08 mg/mL (2.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.66%

    SDS (393 KB)

    COA (263 KB) HNMR (188 KB) RP-HPLC (259 KB) MS (68 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.1591 mL 5.7955 mL 11.5910 mL 28.9774 mL
    5 mM 0.2318 mL 1.1591 mL 2.3182 mL 5.7955 mL
    10 mM 0.1159 mL 0.5795 mL 1.1591 mL 2.8977 mL
    15 mM 0.0773 mL 0.3864 mL 0.7727 mL 1.9318 mL
    20 mM 0.0580 mL 0.2898 mL 0.5795 mL 1.4489 mL
    25 mM 0.0464 mL 0.2318 mL 0.4636 mL 1.1591 mL
    30 mM 0.0386 mL 0.1932 mL 0.3864 mL 0.9659 mL
    40 mM 0.0290 mL 0.1449 mL 0.2898 mL 0.7244 mL
    50 mM 0.0232 mL 0.1159 mL 0.2318 mL 0.5795 mL
    60 mM 0.0193 mL 0.0966 mL 0.1932 mL 0.4830 mL
    80 mM 0.0145 mL 0.0724 mL 0.1449 mL 0.3622 mL
    100 mM 0.0116 mL 0.0580 mL 0.1159 mL 0.2898 mL
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    Sennoside A Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Sennoside A81-27-6DNA/RNA SynthesisHIVHuman immunodeficiency virusAntiviral activityDual inhibitorHIV-1Plant extractsReverse transcriptaseInhibitorinhibitorinhibit

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