Trequinsin hydrochloride (Synonyms: HL 725)

Cat. No.: HY-18740A Purity: 99.93%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Trequinsin hydrochloride (HL 725) is an extremely potent inhibitor of platelet CAMP phosphodiesterase (PDE), with an IC₅₀ of 0.25 nM. Trequinsin hydrochloride (HL 725) is an extremely potent inhibitor of the aggregation of human platelets induced in vitro by ADP, collagen, thrombin and epinephrine.

For research use only. We do not sell to patients.

Trequinsin hydrochloride Chemical Structure

CAS No. : 78416-81-6

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5 mg	USD 165	In-stock	Estimated Time of Arrival: December 31
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25 mg	USD 605	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 990	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 1650	In-stock	Estimated Time of Arrival: December 31
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PDE1 PDE2 PDE3 PDE4 PDE5 PDE6 PDE7 PDE9 PDE10 PDE11 PDE12 PDE8 Autotaxin

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Trequinsin hydrochloride exerts besides its cardiovascular and antihypertensive qualities very potent antiplatelet activities^[1].
Trequinsin hydrochloride is an efficacious agonist of [Ca²⁺]_i^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Samples from healthy volunteer research donors with normal sperm motility parameters in agreement with World Health Organization 2010 criteria.
Concentration:	10 μM.
Incubation Time:	20 min.
Result:	Caused a concentrationdependent increase in [Ca²⁺]_i (EC₅₀ = 6.4 μM [95% confidence interval (CI): 4.1-9.9 μM]).

Molecular Weight

441.95

Formula

C₂₄H₂₈ClN₃O₃

CAS No.

78416-81-6

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(N(CCC1=C2C=C(OC)C(OC)=C1)C2=C/3)N(C)C3=N\C4=C(C)C=C(C)C=C4C.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (141.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (113.13 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.2627 mL	11.3135 mL	22.6270 mL
5 mM	0.4525 mL	2.2627 mL	4.5254 mL
10 mM	0.2263 mL	1.1313 mL	2.2627 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (4.71 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.93%

Select Batch:

Data Sheet (277 KB) SDS (252 KB)

COA (253 KB) LCMS (90 KB)

Handling Instructions (2659 KB)

References

[1]. D Ruppert,, et al. HL 725, an extremely potent inhibitor of platelet phosphodiesterase and induced platelet aggregation in vitro. Life Sci. 1982 Nov 8;31(19):2037-43. [Content Brief]

[2]. K C Agarwal, et al. Role of plasma adenosine in the antiplatelet action of HL 725, a potent inhibitor of cAMP phosphodiesterase: species differences. Thromb Res. 1987 Jul 15;47(2):191-200. [Content Brief]

[3]. Rachel C McBrinn, et al. Novel pharmacological actions of trequinsin hydrochloride improve human sperm cell motility and function. Br J Pharmacol. 2019 Dec;176(23):4521-4536. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.2627 mL	11.3135 mL	22.6270 mL	56.5675 mL
	5 mM	0.4525 mL	2.2627 mL	4.5254 mL	11.3135 mL
	10 mM	0.2263 mL	1.1313 mL	2.2627 mL	5.6567 mL
	15 mM	0.1508 mL	0.7542 mL	1.5085 mL	3.7712 mL
	20 mM	0.1131 mL	0.5657 mL	1.1313 mL	2.8284 mL
	25 mM	0.0905 mL	0.4525 mL	0.9051 mL	2.2627 mL
	30 mM	0.0754 mL	0.3771 mL	0.7542 mL	1.8856 mL
	40 mM	0.0566 mL	0.2828 mL	0.5657 mL	1.4142 mL
	50 mM	0.0453 mL	0.2263 mL	0.4525 mL	1.1313 mL
	60 mM	0.0377 mL	0.1886 mL	0.3771 mL	0.9428 mL
	80 mM	0.0283 mL	0.1414 mL	0.2828 mL	0.7071 mL
	100 mM	0.0226 mL	0.1131 mL	0.2263 mL	0.5657 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Trequinsin hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Trequinsin78416-81-6HL 725HL725HL-725Phosphodiesterase (PDE)Ca2+cAMPPDE3plateletADPcollagenthrombinepinephrineInhibitorinhibitorinhibit

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