Cat. No.: HY-W002199

6:2 Fluorotelomer alcohol

(Synonyms: 6:2 FTOH; 1H,1H,2H,2H-Perfluoro-1-octanol; 2-(Perfluorohexyl)ethanol)

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6:2 Fluorotelomer alcohol (6:2 FTOH) is an orally active, blood-brain barrier-permeable modulator of cyclin D1 and ETS1. 6:2 Fluorotelomer alcohol downregulates cyclin D1 expression, upregulates ETS1 via the TNF-α/ERK 1/2 pathway, impairs mitochondrial membrane potential and respiratory function, increases reactive oxygen species levels, disrupts calcium homeostasis and activates endoplasmic reticulum stress markers, and induces cell proliferation inhibition and endothelial-mesenchymal transition. Furthermore, 6:2 Fluorotelomer alcohol induces morphological abnormalities in zebrafish embryos and liver developmental damage, while disrupting the brain immune microenvironment in mice, causing systemic toxicity and delayed pup maturation in CD-1 mice. 6:2 Fluorotelomer alcohol also induces cortical neuron apoptosis, glial cell activation, synaptic abnormalities, colonic barrier damage, intestinal dysbiosis and autism spectrum disorder-like symptoms in mice. 6:2 Fluorotelomer alcohol shows no mutagenic, clastogenic, primary skin/eye irritation or skin sensitizing effects, exhibits no selective reproductive toxicity in CD-1 mice, and is classified as GHS Category 4 for acute oral toxicity. 6:2 Fluorotelomer alcohol can be used in studies of neurodevelopmental disorders and autism spectrum disorders.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 647-42-7
Formula: C₈H₅F₁₃O
Molecular Weight:364.10

Storage:

Store at room temperature 3 years.

In solvent -80°C, 2 years , -20°C, 1 year

6:2 Fluorotelomer alcohol Chemical Structure

CAS No.: 647-42-7

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Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All ERK Isoforms :

All TNF Receptor Isoforms :

In Vitro

6:2 FTOH (50-200 μg/mL) dose-dependently inhibits proliferation and downregulates cyclin D1 expression in HaCaT human keratinocytes and HDF human dermal fibroblasts, with cyclin D1 reduced at 200 μg/mL in HaCaT cells and at 100 and 200 μg/mL in HDF cells^[1].
6:2 FTOH (50-200 μg/mL) activates ER stress signaling in HaCaT human keratinocytes and HDF human dermal fibroblasts, with dose-dependent increases in GRP78/BiP and CHOP in both cell types, and cell-specific phospho-eIF2α regulation^[1].
6:2 Fluorotelomer alcohol (0.1-1 μM; 24 h) significantly increases the viability of mouse cerebral microvascular endothelial bEnd.3 cells^[2].
6:2 Fluorotelomer alcohol (0.1-1 μM) significantly increases the proliferation rate of mouse cerebral microvascular endothelial bEnd.3 cells, as measured by EdU incorporation^[2].
6:2 Fluorotelomer alcohol (0.1-1 μM) reduces tight junction protein (ZO-1) expression, increases Occludin degradation, upregulates mesenchymal marker (N-cadherin) expression, and downregulates endothelial marker (VE-cadherin) expression in mouse cerebral microvascular endothelial bEnd.3 cells, indicating EndMT induction and tight junction disruption^[2].
6:2 Fluorotelomer alcohol (0.1-1 μM) increases the expression of glycolysis-related proteins (GLUT1, GLUT3, LDHA) in mouse cerebral microvascular endothelial bEnd.3 cells, confirming enhanced glycolysis^[2].
6:2 Fluorotelomer alcohol (0.1-1 μM) significantly upregulates the mRNA expression of inflammation-related genes (IL-1β, TNF-α, COX-2, iNOS) in mouse cerebral microvascular endothelial bEnd.3 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

mouse cerebral microvascular endothelial bEnd.3 cells
Concentration:

0.1-1 μM
Incubation Time:

24 h
Result:

Significantly increased bEnd.3 cell viability at both tested concentrations, with statistically significant elevations relative to control.

In Vivo

6:2 FTOH (aqueous exposure; continuous exposure; 24 hours to 96 hpf) induces dose-dependent developmental toxicity in zebrafish (Danio rerio) embryos, including morphological defects, apoptosis, oxidative stress, and impaired liver development. Significant effects are observed at doses as low as 50 μg/mL, with strong toxicity observed at 200 μg/mL^[1]
6:2 Fluorotelomer alcohol (5-125 mg/kg, oral administration daily; from gestational day 8.5 to delivery) impairs blood-brain barrier function in male BALB/c mouse offspring, alters the brain immune microenvironment, and induces endothelial-mesenchymal transition in brain microvascular endothelial cells via upregulating ETS1^[2].
The no-observed-adverse-effect level (NOAEL) for systemic toxicity of 6:2 fluorotelomer alcohol (100 mg/kg/day; oral administration daily) is 25 mg/kg/day in male mice, 5 mg/kg/day in female mice, >100 mg/kg/day for reproductive toxicity, and 25 mg/kg/day for offspring survival and growth. Its primary target organ is the liver^[3].
6:2 Fluorotelomer alcohol (25 mg/kg/day; oral gavage; once daily; from gestational day 8.5 to parturition) induces autism spectrum disorder-like behaviors, brain histopathological damage, intestinal barrier dysfunction, and gut microbiota dysbiosis in male mouse offspring via the microbiota-gut-brain axis^[4].
6:2 Fluorotelomer alcohol (175-5000 mg/kg; p.o./dermal; single or 90-day exposure) exhibits acute oral toxicity with an LD₅₀ of 1750 mg/kg; its acute dermal LD₅₀ is >5000 mg/kg; subchronic oral toxicity occurs in brown rats at ≥25 mg/kg/day; its no-observed-adverse-effect level (NOAEL) is 5 mg/kg/day^[5].
6:2 Fluorotelomer alcohol (up to 100%) does not induce skin sensitization in mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Danio rerio; Tg(fabp10a;dsRed)^[1]
Dosage:

50 µg/mL; 100 µg/mL; 200 µg/mL (general developmental toxicity); 200 µg/mL (liver development assessment)
Administration:

aqueous exposure; continuous; 24 hours (starting at 24 hpf, general developmental toxicity); continuous until 96 hpf (liver development assessment)
Result:

Caused significant reduction in eye size, significant increase in yolk size, marked reduction in brain size, shortened heartbeat interval, and significantly enlarged heart size at 200 µg/mL.
Induced a significant increase in acridine orange-positive apoptotic/necrotic cells in the head and yolk regions, and significantly elevated ROS levels in embryos at 200 µg/mL.
Caused a marked reduction in DsRed fluorescence intensity and visibly smaller liver field in Tg(fabp10a;dsRed) embryos, with significant decreases in both liver fluorescence intensity and liver size compared to controls at 200 µg/mL.
Caused a significant increase in yolk size at 50 µg/mL.

Chemical Information

CAS No. 647-42-7
Appearance Liquid (Density: 1.65 g/cm³)
Molecular Weight 364.10
Formula C₈H₅F₁₃O
Color Colorless to light yellow
SMILES

OCCC(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F
Synonyms

6:2 FTOH; 1H,1H,2H,2H-Perfluoro-1-octanol; 2-(Perfluorohexyl)ethanol
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Store at room temperature 3 years

In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (274.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7465 mL

13.7325 mL

27.4650 mL

5 mM

0.5493 mL

2.7465 mL

5.4930 mL

10 mM

0.2746 mL

1.3732 mL

2.7465 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lee J Y, et al. PFAS 6: 2 Fluorotelomer Alcohol Disrupts Mitochondrial and Endoplasmic Reticulum Homeostasis in Human Skin Cells and Induces Developmental Toxicity in Zebrafish[J]. Available at SSRN 5990041.

[2]. Xia Y, et al. Embryonic 6:2 Fluorotelomer Alcohol Exposure Disrupts the Blood‒Brain Barrier by Causing Endothelial‒to‒Mesenchymal Transition in the Male Mice. Mol Neurobiol. 2025;62(4):4203-4220. [Content Brief]

[3]. Mukerji P, et al. Oral repeated-dose systemic and reproductive toxicity of 6:2 fluorotelomer alcohol in mice. Toxicol Rep. 2014;2:130-143. Published 2014 Dec 15. [Content Brief]

[4]. Xia Y, et al. Embryonic exposure to 6:2 fluorotelomer alcohol mediates autism spectrum disorder-like behavior by dysfunctional microbe-gut-brain axis in mice. J Hazard Mater. 2025;484:136739. [Content Brief]

[5]. Serex T, et al. Toxicological evaluation of 6:2 fluorotelomer alcohol. Toxicology. 2014;319:1-9. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7465 mL	13.7325 mL	27.4650 mL	68.6625 mL
	5 mM	0.5493 mL	2.7465 mL	5.4930 mL	13.7325 mL
	10 mM	0.2746 mL	1.3732 mL	2.7465 mL	6.8662 mL
	15 mM	0.1831 mL	0.9155 mL	1.8310 mL	4.5775 mL
	20 mM	0.1373 mL	0.6866 mL	1.3732 mL	3.4331 mL
	25 mM	0.1099 mL	0.5493 mL	1.0986 mL	2.7465 mL
	30 mM	0.0915 mL	0.4577 mL	0.9155 mL	2.2887 mL
	40 mM	0.0687 mL	0.3433 mL	0.6866 mL	1.7166 mL
	50 mM	0.0549 mL	0.2746 mL	0.5493 mL	1.3732 mL
	60 mM	0.0458 mL	0.2289 mL	0.4577 mL	1.1444 mL
	80 mM	0.0343 mL	0.1717 mL	0.3433 mL	0.8583 mL
	100 mM	0.0275 mL	0.1373 mL	0.2746 mL	0.6866 mL

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6:2 Fluorotelomer alcohol

Bioactive Compound Library Max

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Anti-Infection Compound Library

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All ERK Isoforms

All TNF Receptor Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

6:2 Fluorotelomer alcohol Related Classifications

HY-W002199 Related Classifications