Cat. No.: HY-N0880

Cinobufotalin

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Cinobufotalin is a cardiotonic steroids or bufadienolides, is extracted from the skin secretions of the giant toads. Cinobufotalin has been used as a cardiotonic, diuretic and a hemostatic agent, Cinobufotalin is also a potential anti-lung cancer agent.

For research use only. We do not sell to patients.

Purity: 99.82%
CAS No.: 1108-68-5
Formula: C₂₆H₃₄O₇
Molecular Weight:458.54

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Cinobufotalin Chemical Structure

CAS No.: 1108-68-5

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
Bel-7402	IC₅₀	1.21 μM Compound: 4	Cytotoxicity against human Bel7402 cells by MTT assay Cytotoxicity against human Bel7402 cells by MTT assay	[PMID: 18558746]
Bel-7402	IC₅₀	8.62 μM Compound: M-2, 5-HCB	Cytotoxicity against human Bel7402 cells assessed as againsthibition of cell growth after 72 hrs Cytotoxicity against human Bel7402 cells assessed as againsthibition of cell growth after 72 hrs	[PMID: 21205911]
HL-60	IC₅₀	0.047 μg/mL Compound: 14	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 11575946]
KB	IC₅₀	0.37 μg/mL Compound: 14	Cytotoxicity against human KB cells after 72 hrs by MTT assay Cytotoxicity against human KB cells after 72 hrs by MTT assay	[PMID: 11575946]
MH60	IC₅₀	>25 μg/mL Compound: 14	Cytotoxicity against mouse MH60 cells after 72 hrs by MTT assay Cytotoxicity against mouse MH60 cells after 72 hrs by MTT assay	[PMID: 11575946]

In Vitro

Cinobufotalin (0.1-10 μM; 72 hours; A549, H460 and HTB-58 human lung cancer cells) treatment induces cytotoxic effect against lung cancer cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[1]

Cell Line:

A549, H460 and HTB-58 human lung cancer cells
Concentration:

0.1 µM, 0.5 µM, 1 µM, 5 µM, 10 µM
Incubation Time:

72 hours
Result:

Significantly induced cell death in a concentration-dependent manner.

In Vivo

Cinobufotalin (1-5 mg/kg; intraperitoneal injection; twice daily; for 1 weeks; male nude mice) treatment inhibits A549 lung cancer cell growth in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male nude mice (4-6 weeks old, BALB/c) with A549 cells^[1]
Dosage:

1 mg/kg or 5 mg/kg
Administration:

Intraperitoneal injection; twice daily; for 1 weeks
Result:

Inhibited A549 lung cancer cell growth in vivo.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 1108-68-5
Appearance Solid
Molecular Weight 458.54
Formula C₂₆H₃₄O₇
Color White to off-white
SMILES

C[C@]([C@@H](C(C=C1)=COC1=O)[C@H]2OC(C)=O)(CC[C@@]3([H])[C@@]4([H])CC[C@@]5(O)[C@@]3(CC[C@H](O)C5)C)[C@@]64[C@@H]2O6
Structure Classification
- Steroids
Initial Source
- Animals
toad
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All In Vivo Efficacy StudyFlow CytometryWBRT-PCR

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Journal Impact Factor

J Adv Res

Lactobacillus plantarum AR113 alleviates Western Diet-Induced colitis and liver injury via bidirectional modulation of the Intestinal-Hepatic FXR signaling axis. [Abstract]

2026 Feb 21:S2090-1232(26)00180-3. PMID: 41730412
Cell Death Dis

Targeting PPARγ via SIAH1/2-mediated ubiquitin-proteasomal degradation as a new therapeutic approach in luminal-type bladder cancer. [Abstract]

2024 Dec 18;15(12):908. PMID: 39695138

Cinobufotalin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 18;15(12):908. [Abstract]

Tumor sizes were measured every 2 days during treatment with cinobufotalin (1 and 5 mg/kg, ip, qd) or cisplatin.

Cinobufotalin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 18;15(12):908. [Abstract]

Effects of cinobufotalin (0-1.25 μM) on cell cycle distribution in RT112 cells. Cells were treated with different concentrations of cinobufotalin for 48 h and subsequently stained using propidium iodide. The cell cycle was analysed via flow cytometry.

Cinobufotalin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 18;15(12):908. [Abstract]

Effect of cinobufotalin (0-1.25 μM) on apoptosis in RT112 cells. Cells were treated with varying concentrations of cinobufotalin for 48 h, stained with Annexin V/7-AAD, and analysed via flow cytometry.

Cinobufotalin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 18;15(12):908. [Abstract]

RT112 cells were treated with the indicated concentrations of cinobufotalin (0-1.25 μM) for 24 h and 48 h and expression of proteins analysed via western blot. Band intensities of each protein were quantified using Image J and normalized to GAPDH.

Cinobufotalin purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2024 Dec 18;15(12):908. [Abstract]

Effects of cinobufotalin (0-1.25 μM) on mRNA levels of PPARγ and its downstream target genes in RT112 cells. Cells were treated with the indicated concentrations of cinobufotalin for 24 h and mRNA expression determined via RT-qPCR.
Pharmacol Res

Small molecule targeting topoisomerase 3β for cancer therapy. [Abstract]

2021 Dec:174:105927. PMID: 34740818
Phytomedicine

Compound-composed Chinese medicine of Huachansu triggers apoptosis of gastric cancer cells through increase of reactive oxygen species levels and suppression of proteasome activities. [Abstract]

2024 Jan:123:155169. PMID: 37992493
Eur J Pharmacol

Novel SREBP1 inhibitor cinobufotalin suppresses proliferation of hepatocellular carcinoma by targeting lipogenesis. [Abstract]

2021 Sep 5:906:174280. PMID: 34174265
J Cell Mol Med

Cinobufotalin Ameliorates the Development of Pulmonary Fibrosis by Suppressing the TGF-β/Smad Pathway via Regulating PI15. [Abstract]

2025 Aug;29(16):e70788. PMID: 40845143

Solvent & Solubility

In Vitro:

DMSO : 87.5 mg/mL (190.82 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.1808 mL

10.9042 mL

21.8083 mL

5 mM

0.4362 mL

2.1808 mL

4.3617 mL

10 mM

0.2181 mL

1.0904 mL

2.1808 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.17 mg/mL (4.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.17 mg/mL (4.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.17 mg/mL (4.73 mM); Clear solution

This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (278 KB)
SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kai S, et al. Pre-clinical evaluation of cinobufotalin as a potential anti-lung cancer agent. Biochem Biophys Res Commun. 2014 Sep 26;452(3):768-74. [Content Brief]

[2]. Li C, Guo H, Wang C, et al. Network pharmacological mechanism of Cinobufotalin against glioma. Prog Brain Res. 2021;265:119-137. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.1808 mL	10.9042 mL	21.8083 mL	54.5209 mL
	5 mM	0.4362 mL	2.1808 mL	4.3617 mL	10.9042 mL
	10 mM	0.2181 mL	1.0904 mL	2.1808 mL	5.4521 mL
	15 mM	0.1454 mL	0.7269 mL	1.4539 mL	3.6347 mL
	20 mM	0.1090 mL	0.5452 mL	1.0904 mL	2.7260 mL
	25 mM	0.0872 mL	0.4362 mL	0.8723 mL	2.1808 mL
	30 mM	0.0727 mL	0.3635 mL	0.7269 mL	1.8174 mL
	40 mM	0.0545 mL	0.2726 mL	0.5452 mL	1.3630 mL
	50 mM	0.0436 mL	0.2181 mL	0.4362 mL	1.0904 mL
	60 mM	0.0363 mL	0.1817 mL	0.3635 mL	0.9087 mL
	80 mM	0.0273 mL	0.1363 mL	0.2726 mL	0.6815 mL
	100 mM	0.0218 mL	0.1090 mL	0.2181 mL	0.5452 mL

Keywords

Cinobufotalin

6 Cited Publications

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Cinobufotalin

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Publications Citing Use of MedChemExpress (MCE) Cinobufotalin

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Cinobufotalin Related Classifications

HY-N0880 Related Classifications