1. Fluorescent Dye
  2. Fluorescence Coupling Reagent
    Labeling Chemistry
    Fluorescence Wavelength Division
  3. Amine Reactive
    Cyanine
    Ex 570-590 nm Yellow
    Em 570-590 nm Yellow
  4. Cy3 NHS ester

Cy3 NHS ester  (Synonyms: Cyanine3 NHS ester)

Cat. No.: HY-112498 Purity: 99.05%
COA Handling Instructions

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

For research use only. We do not sell to patients.

Cy3 NHS ester Chemical Structure

Cy3 NHS ester Chemical Structure

CAS No. : 1393363-07-9

Size Price Stock Quantity
10 mg USD 280 In-stock
Estimated Time of Arrival: December 31
25 mg USD 400 In-stock
Estimated Time of Arrival: December 31
50 mg USD 690 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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Description

Cy3 NHS ester (Cyanine3 NHS ester) is a yellow emitting fluorescent dye for labeling amino-groups in biomolecules. Used to label soluble proteins, peptides, and oligonucleotides/DNA.

Molecular Weight

590.15

Formula

C34H40ClN3O4

CAS No.
Emission (Em)
Excitation (Ex)
SMILES

CC1(C)C(/C=C/C=C2N(C)C3=C(C=CC=C3)C/2(C)C)=[N+](CCCCCC(ON4C(CCC4=O)=O)=O)C5=C1C=CC=C5.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under argon

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under argon)

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (423.62 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6945 mL 8.4724 mL 16.9448 mL
5 mM 0.3389 mL 1.6945 mL 3.3890 mL
10 mM 0.1694 mL 0.8472 mL 1.6945 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (3.52 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Cy3 NHS ester
Cat. No.:
HY-112498
Quantity:
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