Diclofenac Sodium (Synonyms: GP 45840)

Name: Diclofenac Sodium
Brand: MedChemExpress
SKU: HY-15037
Rating: 5.0 (24 reviews)

Cat. No.: HY-15037 Purity: 99.81%: Data Sheet
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Diclofenac Sodium (GP 45840) is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC₅₀s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells, and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively. Diclofenac Sodium induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade.

For research use only. We do not sell to patients.

CAS No. : 15307-79-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
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Based on 24 publication(s) in Google Scholar

Other Forms of Diclofenac Sodium:

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Diclofenac Sodium (Standard) In-stock
Diclofenac-¹³C₆ sodium heminonahydrate Get quote

24 Publications Citing Use of MCE Diclofenac Sodium

Cell Proliferation/Viability Assay

Flow Cytometry

Cell Imaging/Staining

: Diclofenac Sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066.; KATOIII and KATO/DDP were treated with different concentration of Diclofenac (DCF) (0–1000 µM) for 48 h, and cell viability was assessed.

: Diclofenac Sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066.; The KATO/DDP cells were treated with Cisplatin alone or cisplatin in combination with Diclofenac (DCF) at 10 or 20 µM for 24 h, at 37 °C. The apoptosis cells were detected by using annexin V and PI staining and analyzed by flow cytometer.

: Diclofenac Sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066.; The combination effect of Diclofenac (DCF) (0, 5, 10, 20 μM) and Cisplatin on the expression of cleaved PARP in KATO/DDP cells was examined by Western blot analysis.

: Diclofenac Sodium purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 11;23(20):12066.; The formation of autophagic vacuoles in KATO/DDP cells increased from 27% to 39% and 45% in cotreatments involving cisplatin and Diclofenac (DCF) at 10 and 20 μM, respectively, when compared with treatment of cisplatin alone.

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COX COX-1 COX-2 COX-3

Biological Activity
Purity & Documentation
References
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Description

Diclofenac Sodium (GP 45840) is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC₅₀s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells^[1], and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively^[2]. Diclofenac Sodium induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade^[3].

IC₅₀ & Target^[1]^[2]

Human COX-2

1.3 nM (IC₅₀, in CHO cells)

Human COX-1

4 nM (IC₅₀, in CHO cells)

Ovine COX-2

0.84 μM (IC₅₀)

Ovine COX-1

5.1 μM (IC₅₀)

Cellular Effect

Cell Line	Type	Value	Description	References
Erythrocyte	EC₅₀	11.7 μg/mL Compound: Diclofenac sodium	Anti-inflammatory activity in human red blood cells assessed as stabilization of cell membrane Anti-inflammatory activity in human red blood cells assessed as stabilization of cell membrane	[PMID: 32485533]
HT-29	IC₅₀	137.1 μM Compound: Diclofenac sodium	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay	[PMID: 32485532]
RAW264.7	IC₅₀	10.27 μM Compound: Diclofenac sodium	Inhibition of LPS-induced TNFalpha production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA Inhibition of LPS-induced TNFalpha production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA	[PMID: 33892270]
RAW264.7	IC₅₀	12.19 μM Compound: Diclofenac sodium	Inhibition of LPS-induced 15(S)-HETE production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA Inhibition of LPS-induced 15(S)-HETE production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA	[PMID: 33892270]
RAW264.7	IC₅₀	12.91 μM Compound: Diclofenac sodium	Inhibition of LPS-induced IL-6 production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA Inhibition of LPS-induced IL-6 production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by ELISA	[PMID: 33892270]
RAW264.7	IC₅₀	25.51 μM Compound: Diclofenac sodium	Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced intracellular ROS production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by DCFH-DA staining based fluorescence analysis Antioxidant activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced intracellular ROS production preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by DCFH-DA staining based fluorescence analysis	[PMID: 33892270]
RAW264.7	IC₅₀	331 μM Compound: Diclofenac sodium	Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis Antiinflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based analysis	[PMID: 38805684]
RAW264.7	IC₅₀	36.28 μM Compound: Diclofenac sodium	Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by DAF-FM based fluorescence analysis Inhibition of LPS-induced NO production in mouse RAW264.7 cells preincubated for 2 hrs followed by LPS stimulation and measured after 20 hrs by DAF-FM based fluorescence analysis	[PMID: 33892270]
THP-1	IC₅₀	200.9 μM Compound: Diclofenac sodium	Cytotoxicity against PMA/LPS-challenged human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay Cytotoxicity against PMA/LPS-challenged human THP1 cells assessed as reduction in cell viability incubated for 24 hrs by MTS assay	[PMID: 32485532]

In Vitro

Diclofenac effectively blocks COX-1 mediated prostanoid production from U937 cell microsomes, with an IC₅₀ of 7±3 nM^[1].
Diclofenac (1-60 μM; 1 day) induces neural stem cells (NSCs)death in a concentration-dependent manner^[3].
Diclofenac (10-60 μM; 6 hours) increases the expression of cleaved (activated) caspase-3^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Neural stem cells (NSCs)
Concentration:	1, 3, 10, 30, 60 μM
Incubation Time:	1 day
Result:	Induction of cell death was concentration-dependent and the effect was not saturated at a concentration of up to 60 μM.

Western Blot Analysis^[3]

Cell Line:	Neural stem cells (NSCs)
Concentration:	10, 30 or 60 μM
Incubation Time:	6 hours
Result:	The activation of caspase-3 was increased in a concentration-dependent manner.

In Vivo

Diclofenac (3 mg/kg, b.i.d., for 5 days) significantly increases faecal ⁵¹Cr excretion in rats, and such effect is also observed in squirrel monkeys after administrated of 1 mg/kg twice daily for 4 days^[1].
Diclofenac (10 mg/kg; administered via oral route just prior to induction of inflammation) shows in vivo anti-inflammatory activity in Wistar rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (150±200 g)^[1]
Dosage:	3 mg/kg
Administration:	Oral administration, b.i.d., for 5 days
Result:	Resulted in a significant increase in faecal ⁵¹Cr excretion.

Animal Model:	Wistar rats (150-175 g) bearing Formalin-induced rat foot paw edema model^[2]
Dosage:	10 mg/kg
Administration:	Administered via oral route just prior to induction of inflammation
Result:	Showed in vivo anti-inflammatory activity (% edema inhibition=29.2, 1 h; 22.2, 3 h; 20, 6 h).

Molecular Weight

318.13

Formula

C₁₄H₁₀Cl₂NNaO₂

CAS No.

15307-79-6

Appearance

Solid

Color

White to off-white

SMILES

O=C(O[Na])CC1=CC=CC=C1NC2=C(Cl)C=CC=C2Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (392.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 10 mg/mL (31.43 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1434 mL	15.7168 mL	31.4337 mL
5 mM	0.6287 mL	3.1434 mL	6.2867 mL
10 mM	0.3143 mL	1.5717 mL	3.1434 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 4.55 mg/mL (14.30 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (283 KB) SDS (728 KB)

COA (255 KB) RP-HPLC (247 KB)

Handling Instructions (2659 KB)

References

[1]. Riendeau D, et al. Biochemical and pharmacological profile of a tetrasubstituted furanone as a highly selective COX-2 inhibitor. Br J Pharmacol. 1997 May;121(1):105-17. [Content Brief]

[2]. Labib MB, et al. Design, synthesis of novel isoindoline hybrids as COX-2 inhibitors: Anti-inflammatory, analgesic activities and docking study. Bioorg Chem. 2018 Oct;80:70-80. [Content Brief]

[3]. Chiho Kudo, et al. Diclofenac Inhibits Proliferation and Differentiation of Neural Stem Cells. Biochem Pharmacol. 2003 Jul 15;66(2):289-95. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1434 mL	15.7168 mL	31.4337 mL	78.5842 mL
	5 mM	0.6287 mL	3.1434 mL	6.2867 mL	15.7168 mL
	10 mM	0.3143 mL	1.5717 mL	3.1434 mL	7.8584 mL
	15 mM	0.2096 mL	1.0478 mL	2.0956 mL	5.2389 mL
	20 mM	0.1572 mL	0.7858 mL	1.5717 mL	3.9292 mL
	25 mM	0.1257 mL	0.6287 mL	1.2573 mL	3.1434 mL
	30 mM	0.1048 mL	0.5239 mL	1.0478 mL	2.6195 mL
DMSO	40 mM	0.0786 mL	0.3929 mL	0.7858 mL	1.9646 mL
	50 mM	0.0629 mL	0.3143 mL	0.6287 mL	1.5717 mL
	60 mM	0.0524 mL	0.2619 mL	0.5239 mL	1.3097 mL
	80 mM	0.0393 mL	0.1965 mL	0.3929 mL	0.9823 mL
	100 mM	0.0314 mL	0.1572 mL	0.3143 mL	0.7858 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Diclofenac Sodium Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Diclofenac Sodium (Synonyms: GP 45840)

Customer Review

Other Forms of Diclofenac Sodium:

Diclofenac Sodium Related Antibodies

24 Publications Citing Use of MCE Diclofenac Sodium

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•Related Screening Libraries:

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View All COX Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Diclofenac Sodium Related Antibodies

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Complete Stock Solution Preparation Table

Diclofenac Sodium Related Classifications

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