Diclofenac diethylamine

Cat. No.: HY-15036A Purity: 99.96%: FAQs
Data Sheet SDS COA Handling Instructions

Diclofenac diethylamine is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC₅₀s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells, and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively. Diclofenac diethylamine induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade.

For research use only. We do not sell to patients.

Diclofenac diethylamine Chemical Structure

CAS No. : 78213-16-8

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Solution
10 mM * 1 mL in DMSO	USD 61	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL ready for reconstitution	USD 61	In-stock	Estimated Time of Arrival: December 31
Solid
500 mg	USD 55	In-stock	Estimated Time of Arrival: December 31
5 g	USD 68	In-stock	Estimated Time of Arrival: December 31
10 g	USD 111	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 12 publication(s) in Google Scholar

Other Forms of Diclofenac diethylamine:

Diclofenac In-stock
Diclofenac-d₄ In-stock
Diclofenac Sodium In-stock
Diclofenac potassium In-stock

12 Publications Citing Use of MCE Diclofenac diethylamine

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Biological Activity
Purity & Documentation
References
Customer Review

Description

Diclofenac diethylamine is a potent and nonselective anti-inflammatory agent, acts as a COX inhibitor, with IC₅₀s of 4 and 1.3 nM for human COX-1 and COX-2 in CHO cells^[1], and 5.1 and 0.84 μM for ovine COX-1 and COX-2, respectively^[2]. Diclofenac diethylamine induces apoptosis of neural stem cells (NSCs) via the activation of the caspase cascade^[3].

IC₅₀ & Target^[1]^[2]

Human COX-2

1.3 nM (IC₅₀, in CHO cells)

Human COX-1

4 nM (IC₅₀, in CHO cells)

Ovine COX-2

0.84 μM (IC₅₀)

Ovine COX-1

5.1 μM (IC₅₀)

In Vitro

Diclofenac effectively blocks COX-1 mediated prostanoid production from U937 cell microsomes, with an IC₅₀ of 7±3 nM^[1].
Diclofenac (1-60 μM; 1 day) induces neural stem cells (NSCs)death in a concentration-dependent manner^[3].
Diclofenac (10-60 μM; 6 hours) increases the expression of cleaved (activated) caspase-3^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:	Neural stem cells (NSCs)
Concentration:	1, 3, 10, 30, 60 μM
Incubation Time:	1 day
Result:	Induction of cell death was concentration-dependent and the effect was not saturated at a concentration of up to 60 μM.

Western Blot Analysis^[3]

Cell Line:	Neural stem cells (NSCs)
Concentration:	10, 30 or 60 μM
Incubation Time:	6 hours
Result:	The activation of caspase-3 was increased in a concentration-dependent manner.

In Vivo

Diclofenac (3 mg/kg, b.i.d., for 5 days) significantly increases faecal ⁵¹Cr excretion in rats, and such effect is also observed in squirrel monkeys after administrated of 1 mg/kg twice daily for 4 days^[1].
Diclofenac (10 mg/kg; administered via oral route just prior to induction of inflammation) shows in vivo anti-inflammatory activity in Wistar rats^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley rats (150±200 g)^[1]
Dosage:	3 mg/kg
Administration:	Oral administration, b.i.d., for 5 days
Result:	Resulted in a significant increase in faecal ⁵¹Cr excretion.

Animal Model:	Wistar rats (150-175 g) bearing Formalin-induced rat foot paw edema model^[2]
Dosage:	10 mg/kg
Administration:	Administered via oral route just prior to induction of inflammation
Result:	Showed in vivo anti-inflammatory activity (% edema inhibition=29.2, 1 h; 22.2, 3 h; 20, 6 h).

Clinical Trial

Molecular Weight

369.29

Appearance

Solid

Formula

C₁₈H₂₂Cl₂N₂O₂

CAS No.

78213-16-8

SMILES

O=C(O)CC1=CC=CC=C1NC2=C(Cl)C=CC=C2Cl.CCNCC

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 150 mg/mL (406.18 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7079 mL	13.5395 mL	27.0790 mL
5 mM	0.5416 mL	2.7079 mL	5.4158 mL
10 mM	0.2708 mL	1.3539 mL	2.7079 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (6.77 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.77 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (6.77 mM); Clear solution

*All of the co-solvents are available by MCE.

Purity & Documentation

Purity: 99.96%

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Data Sheet (283 KB) SDS (251 KB)

COA (254 KB) LCMS (265 KB)

Handling Instructions (2659 KB)

References

[1]. Riendeau D, et al. Biochemical and pharmacological profile of a tetrasubstituted furanone as a highly selective COX-2 inhibitor. Br J Pharmacol. 1997 May;121(1):105-17. [Content Brief]

[2]. Labib MB, et al. Design, synthesis of novel isoindoline hybrids as COX-2 inhibitors: Anti-inflammatory, analgesic activities and docking study. Bioorg Chem. 2018 Oct;80:70-80. [Content Brief]

[3]. Chiho Kudo, et al. Diclofenac Inhibits Proliferation and Differentiation of Neural Stem Cells. Biochem Pharmacol. 2003 Jul 15;66(2):289-95. [Content Brief]