Cat. No.: HY-17029

Epothilone B

(Synonyms: EPO 906; Patupilone)

8 Cited Publications

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Based on 8 publication(s) in Google Scholar

Epothilone B is a microtubule stabilizer (MSA) with a K_i of 0.71μM, and is a MSA with blood-brain barrier permeability. Epothilone B acts by binding to the αβ-tubulin heterodimer subunit which causes decreasing of αβ-tubulin dissociation.

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Purity: 99.91%
CAS No.: 152044-54-7
Formula: C₂₇H₄₁NO₆S
Molecular Weight:507.68

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Epothilone B Chemical Structure

CAS No.: 152044-54-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (8)
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Bioz

Biological Activity

Description

IC₅₀ & Target

EC0.01: 1.8 μM (Microtubule/Tubulin)^[1]

All Antibiotic Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
1A9	IC₅₀	0.13 nM Compound: 175 (Epothilone B)	Inhibitory concentration against ovarian carcinoma 1A9 cell growth Inhibitory concentration against ovarian carcinoma 1A9 cell growth	[PMID: 16134928]
1A9	IC₅₀	0.06 nM Compound: 11	Cytotoxicity against human 1A9 cells Cytotoxicity against human 1A9 cells	[PMID: 28850227]
1A9/ptx-10	IC₅₀	1 nM Compound: 175 (Epothilone B)	Inhibitory concentration against beta-tubulin mutant 1A9PTX10 cell growth Inhibitory concentration against beta-tubulin mutant 1A9PTX10 cell growth	[PMID: 16134928]
1A9/ptx-22	IC₅₀	0.31 nM Compound: 175 (Epothilone B)	Inhibitory concentration against beta-tubulin mutant 1A9PTX22 cell line Inhibitory concentration against beta-tubulin mutant 1A9PTX22 cell line	[PMID: 16134928]
A2780	EC₅₀	4 nM Compound: Epothilone B	Antiproliferative activity against D4-9-31 resistance human A2780 cells incubated for 72 hrs by ATPlite reagent based assay Antiproliferative activity against D4-9-31 resistance human A2780 cells incubated for 72 hrs by ATPlite reagent based assay	[PMID: 31047749]
A2780	EC₅₀	5.2 nM Compound: Epothilone B	Antiproliferative activity against human A2780 cells incubated for 72 hrs by ATPlite reagent based assay Antiproliferative activity against human A2780 cells incubated for 72 hrs by ATPlite reagent based assay	[PMID: 31047749]
A-431	IC₅₀	7.3 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human A431 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human A431 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
A-431	IC₅₀	7.9 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human A431 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human A431 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
A-431	IC₅₀	6.7 x 10^-5 M Compound: Epothilone B	Cytotoxicity against human A-431 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human A-431 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
A-431	IC₅₀	67 μM Compound: Epothilone B	Cytotoxicity against human A-431 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human A-431 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
A-431	IC₅₀	5.1 x 10^-5 μM Compound: Epothilone B	Antiproliferative activity against human A-431 cells assessed as cell growth inhibition Antiproliferative activity against human A-431 cells assessed as cell growth inhibition	[PMID: 37931226]
A549	IC₅₀	1.5 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human A549 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
A549	GI₅₀	0.7 nM Compound: Epothilone B	Growth inhibition of human A549 cells after 4 days by SRB assay Growth inhibition of human A549 cells after 4 days by SRB assay	[PMID: 28740601]
A549	IC₅₀	4 nM Compound: Epothilone B	Cytotoxicity against human A549 cells measured after 5 days by MTT assay Cytotoxicity against human A549 cells measured after 5 days by MTT assay	[PMID: 31021629]
A549	IC₅₀	4 x 10^-3 μM Compound: Epothilone B	Cytotoxicity against human A549 cells measured after 5 days by MTT assay Cytotoxicity against human A549 cells measured after 5 days by MTT assay	[PMID: 31021629]
A549	IC₅₀	6.9 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
A549	IC₅₀	8.8 x 10^-5 M Compound: Epothilone B	Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
A549	IC₅₀	88 μM Compound: Epothilone B	Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human A549 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
A549	IC₅₀	5.3 x 10^-5 μM Compound: Epothilone B	Antiproliferative activity against human A549 cells assessed as cell growth inhibition Antiproliferative activity against human A549 cells assessed as cell growth inhibition	[PMID: 37931226]
Bel-7402	IC₅₀	0.9 nM Compound: Epothilone B	Antiproliferative activity against human Bel7402 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human Bel7402 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
HCC1937	IC₅₀	>10 μM Compound: Epothilone B	Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay Antiproliferative activity against exponentially growing adherent human HCC1937 cells assessed as inhibition of cell proliferation after 72 hrs by luminescence detection based ATPlite assay	[PMID: 25872984]
HCT-116	IC₅₀	0.8 nM Compound: 2; EPO B	Antiproliferative activity against human HCT116 cells assessed as inhibition of cell proliferation Antiproliferative activity against human HCT116 cells assessed as inhibition of cell proliferation	[PMID: 30149324]
HeLa	IC₅₀	3 nM Compound: 2; EPO B	Cytotoxicity against human HeLa cells Cytotoxicity against human HeLa cells	[PMID: 30149324]
Hep 3B2	IC₅₀	>100 nM Compound: Epothilone B	Antiproliferative activity against human Hep3B after 72 hrs by CellTiter Glo assay Antiproliferative activity against human Hep3B after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
HepG2	EC₅₀	0.01 μM Compound: Epothilone B	Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	EC₅₀	12.6 μM Compound: Epothilone B	Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis	[PMID: 20966043]
HepG2	IC₅₀	44.7 nM Compound: Epothilone B	Antiproliferative activity against human HepG2 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HepG2 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
Hs-578T	IC₅₀	3 nM Compound: 2; EPO B	Cytotoxicity against human Hs 578T cells Cytotoxicity against human Hs 578T cells	[PMID: 30149324]
Huh-7	CC₅₀	>6.25 μM Compound: GNF-Pf-193	NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7) NOVARTIS: Cytotoxicity against human hepatocellular carcinoma cell line (Huh7)	[PMID: 18579783]
Huh-7	IC₅₀	4.5 nM Compound: Epothilone B	Antiproliferative activity against human HuH7 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human HuH7 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
HUVEC	IC₅₀	0.00055 μM Compound: Epothilone B	Cytotoxicity against HUVEC after 5 days by MTT assay Cytotoxicity against HUVEC after 5 days by MTT assay	[PMID: 27231731]
HUVEC	IC₅₀	0.55 nM Compound: Epothilone B	Cytotoxicity against HUVEC after 5 days by MTT assay Cytotoxicity against HUVEC after 5 days by MTT assay	[PMID: 27231731]
KB	IC₅₀	0.18 nM Compound: Epothilone B	Concentration required to inhibit the growth of paclitaxel-resistant human epidermoid carcinoma cells KB-8511 by 50 percent (72 hr exposure) Concentration required to inhibit the growth of paclitaxel-resistant human epidermoid carcinoma cells KB-8511 by 50 percent (72 hr exposure)	[PMID: 11133086]
KB 3-1	IC₅₀	0.19 nM Compound: Epothilone B	Concentration required to inhibit the growth of paclitaxel-sensitive human epidermoid carcinoma cells KB-31 by 50 percent (72 hr exposure) Concentration required to inhibit the growth of paclitaxel-sensitive human epidermoid carcinoma cells KB-31 by 50 percent (72 hr exposure)	[PMID: 11133086]
KB 3-1	IC₅₀	0.00022 μM Compound: Epothilone B	Cytotoxicity against human KB 3.1 cells after 5 days by MTT assay Cytotoxicity against human KB 3.1 cells after 5 days by MTT assay	[PMID: 27231731]
KB 3-1	IC₅₀	0.22 nM Compound: Epothilone B	Cytotoxicity against human KB 3.1 cells after 5 days by MTT assay Cytotoxicity against human KB 3.1 cells after 5 days by MTT assay	[PMID: 27231731]
KB 3-1	IC₅₀	6.1 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human KB3-1 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human KB3-1 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
KB 3-1	IC₅₀	0.22 μM Compound: Epothilon B	Cytotoxicity against human KB 3.1 cells assessed as inhibition of cell growth after 5 days by MTT assay Cytotoxicity against human KB 3.1 cells assessed as inhibition of cell growth after 5 days by MTT assay	[PMID: 29489350]
KB 3-1	IC₅₀	3 nM Compound: 2; EPO B	Cytotoxicity against human KB3-1 cells Cytotoxicity against human KB3-1 cells	[PMID: 30149324]
KB 3-1	IC₅₀	6.5 x 10^-5 μM Compound: Epothilone B	Cytotoxicity in human KB 3.1 cells Cytotoxicity in human KB 3.1 cells	[PMID: 31001977]
KB 3-1	IC₅₀	5.3 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human KB3-1 cells measured after 5 days by MTT assay Cytotoxicity against human KB3-1 cells measured after 5 days by MTT assay	[PMID: 31021629]
KB 3-1	IC₅₀	8.9 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human KB3-1 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human KB3-1 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
KB 3-1	IC₅₀	8.9 x 10^-5 μM Compound: Epothilon B	Cytotoxicity against human KB3.1 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against human KB3.1 cells assessed as reduction in cell viability by MTT assay	[PMID: 32786884]
KB 3-1	IC₅₀	6.9 x 10^-5 M Compound: Epothilone B	Cytotoxicity against human KB 3-1 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human KB 3-1 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
KB 3-1	IC₅₀	69 μM Compound: Epothilone B	Cytotoxicity against human KB 3-1 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human KB 3-1 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
KB 3-1	IC₅₀	3.4 x 10^-5 μM Compound: Epothilone B	Antiproliferative activity against human KB-3-1 cells assessed as cell growth inhibition Antiproliferative activity against human KB-3-1 cells assessed as cell growth inhibition	[PMID: 37931226]
KB 3-1	IC₅₀	8.6 x 10^-6 μg/mL Compound: Epothilone B	Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay Cytotoxicity against human KB-3-1 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay	[PMID: 39141525]
KB-V1	IC₅₀	3 nM Compound: 2; EPO B	Cytotoxicity against human KBV1 cells Cytotoxicity against human KBV1 cells	[PMID: 30149324]
L929	IC₅₀	1 ng/mL Compound: 2	Cytotoxicity against mouse L929 cells Cytotoxicity against mouse L929 cells	[PMID: 11473410]
L929	IC₅₀	1.7 nM Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
L929	IC₅₀	1.7 x 10^-3 μM Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
L929	IC₅₀	1.4 μM Compound: Epothilon B	Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth after 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth after 5 days by MTT assay	[PMID: 29489350]
L929	IC₅₀	0.00275 μM Compound: Epothilone B	Cytotoxicity in mouse L929 cells Cytotoxicity in mouse L929 cells	[PMID: 31001977]
L929	IC₅₀	7.5 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against mouse L929 cells measured after 5 days by MTT assay Cytotoxicity against mouse L929 cells measured after 5 days by MTT assay	[PMID: 31021629]
L929	IC₅₀	1.4 nM Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
L929	IC₅₀	1.4 x 10^-3 μM Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
L929	IC₅₀	2.6 nM Compound: Epothilon B	Cytotoxicity against mouse L929 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell viability by MTT assay	[PMID: 32786884]
L929	IC₅₀	2.6 x 10^-3 μM Compound: Epothilon B	Cytotoxicity against mouse L929 cells assessed as reduction in cell viability by MTT assay Cytotoxicity against mouse L929 cells assessed as reduction in cell viability by MTT assay	[PMID: 32786884]
L929	IC₅₀	1.4 mM Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
L929	IC₅₀	1.4 x 10^-3 M Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
L929	IC₅₀	4.3 x 10^-4 μM Compound: Epothilone B	Antiproliferative activity against mouse L929 cells assessed as cell growth inhibition Antiproliferative activity against mouse L929 cells assessed as cell growth inhibition	[PMID: 37931226]
L929	IC₅₀	8.6 x 10^-5 μg/mL Compound: Epothilone B	Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay Cytotoxicity against mouse L929 cells assessed as inhibition of cell growth incubated for 5 days by MTT assay	[PMID: 39141525]
MCF7	ED₅₀	0.0015 μM Compound: 1b	Cytotoxicity against human MCF7 cells by phototoxicity labeling study Cytotoxicity against human MCF7 cells by phototoxicity labeling study	[PMID: 19428248]
MCF7	IC₅₀	1.5 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
MCF7	IC₅₀	38.17 nM Compound: Epothilone B	Antiproliferative activity against human MCF7 cells after 2 days by MTT assay Antiproliferative activity against human MCF7 cells after 2 days by MTT assay	[PMID: 30318441]
MCF7	IC₅₀	7.4 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human MCF7 cells measured after 5 days by MTT assay Cytotoxicity against human MCF7 cells measured after 5 days by MTT assay	[PMID: 31021629]
MCF7	IC₅₀	2.4 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human MCF7 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human MCF7 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
MCF7	IC₅₀	8.1 x 10^-5 M Compound: Epothilone B	Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
MCF7	IC₅₀	81 μM Compound: Epothilone B	Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human MCF7 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
MCF7	IC₅₀	3 x 10^-5 μM Compound: Epothilone B	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition	[PMID: 37931226]
MHCC97H	IC₅₀	6.7 nM Compound: Epothilone B	Antiproliferative activity against human MHCC97H after 72 hrs by CellTiter Glo assay Antiproliferative activity against human MHCC97H after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
MM1.S	IC₅₀	1 nM Compound: 2; EPO B	Antiproliferative activity against human MM1S cells assessed as inhibition of cell proliferation Antiproliferative activity against human MM1S cells assessed as inhibition of cell proliferation	[PMID: 30149324]
PC-3	IC₅₀	2.5 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
PC-3	GI₅₀	1.7 nM Compound: Epothilone B	Growth inhibition of human PC3 cells after 4 days by SRB assay Growth inhibition of human PC3 cells after 4 days by SRB assay	[PMID: 28740601]
PC-3	IC₅₀	2.2 nM Compound: Epothilone B	Cytotoxicity against human PC3 cells measured after 5 days by MTT assay Cytotoxicity against human PC3 cells measured after 5 days by MTT assay	[PMID: 31021629]
PC-3	IC₅₀	2.2 x 10^-3 μM Compound: Epothilone B	Cytotoxicity against human PC3 cells measured after 5 days by MTT assay Cytotoxicity against human PC3 cells measured after 5 days by MTT assay	[PMID: 31021629]
PC-3	IC₅₀	1.6 nM Compound: Epothilone B	Cytotoxicity against human PC3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
PC-3	IC₅₀	1.6 x 10^-3 μM Compound: Epothilone B	Cytotoxicity against human PC3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human PC3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
PC-3	IC₅₀	7.1 x 10^-5 M Compound: Epothilone B	Cytotoxicity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
PC-3	IC₅₀	71 μM Compound: Epothilone B	Cytotoxicity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human PC-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
PC-3	IC₅₀	0.28 x 10^-5 μM Compound: Epothilone B	Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition Antiproliferative activity against human PC-3 cells assessed as cell growth inhibition	[PMID: 37931226]
PLC-PRF-5	IC₅₀	12.7 nM Compound: Epothilone B	Antiproliferative activity against human PLC/PRF/5 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human PLC/PRF/5 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
PLC-PRF-5	IC₅₀	3.8 nM Compound: Epothilone B	Antiproliferative activity against human PLC/PRF/5 cells Antiproliferative activity against human PLC/PRF/5 cells	[PMID: 22320354]
RPMI-8226	IC₅₀	1 nM Compound: 2; EPO B	Antiproliferative activity against human RPM18226 cells assessed as inhibition of cell proliferation Antiproliferative activity against human RPM18226 cells assessed as inhibition of cell proliferation	[PMID: 30149324]
SK-OV-3	IC₅₀	2.36 x 10^-5 μM Compound: Epothilone B	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]
SK-OV-3	IC₅₀	2.4 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human SKOV3 cells measured after 5 days by MTT assay Cytotoxicity against human SKOV3 cells measured after 5 days by MTT assay	[PMID: 31021629]
SK-OV-3	IC₅₀	2.8 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human SKOV3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell growth incubated for 5 days by MTT assay	[PMID: 31599583]
SK-OV-3	IC₅₀	0.29 mM Compound: Epothilone B	Cytotoxicity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
SK-OV-3	IC₅₀	2.9 x 10^-4 M Compound: Epothilone B	Cytotoxicity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay Cytotoxicity against human SK-OV-3 cells assessed as inhibition of cell proliferation incubated for 5 days by MTT assay	[PMID: 35175766]
SK-OV-3	IC₅₀	1.8 x 10^-4 μM Compound: Epothilone B	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition	[PMID: 37931226]
SMMC-7721	IC₅₀	2.3 nM Compound: Epothilone B	Antiproliferative activity against human SMMC7721 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SMMC7721 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-182	IC₅₀	22.3 nM Compound: Epothilone B	Antiproliferative activity against human SNU182 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU182 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-387	IC₅₀	20 nM Compound: Epothilone B	Antiproliferative activity against human SNU387 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU387 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-398	IC₅₀	2.9 nM Compound: Epothilone B	Antiproliferative activity against human SNU398 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU398 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-398	IC₅₀	5 nM Compound: Epothilone B	Antiproliferative activity against human SNU398 cells Antiproliferative activity against human SNU398 cells	[PMID: 22320354]
SNU-423	IC₅₀	10.3 nM Compound: Epothilone B	Antiproliferative activity against human SNU423 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU423 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-449	IC₅₀	19.9 nM Compound: Epothilone B	Antiproliferative activity against human SNU449 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU449 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
SNU-475	IC₅₀	11.6 nM Compound: Epothilone B	Antiproliferative activity against human SNU475 after 72 hrs by CellTiter Glo assay Antiproliferative activity against human SNU475 after 72 hrs by CellTiter Glo assay	[PMID: 22320354]
U-266	IC₅₀	1 nM Compound: 2; EPO B	Antiproliferative activity against human U266 cells assessed as inhibition of cell proliferation Antiproliferative activity against human U266 cells assessed as inhibition of cell proliferation	[PMID: 30149324]
U2OS	IC₅₀	1.04 x 10^-4 μM Compound: Epothilone B	Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 5 days by MTT assay Cytotoxicity against human U2OS cells assessed as reduction in cell viability after 5 days by MTT assay	[PMID: 28398049]

In Vitro

Epothilone B inhibits HCT116 cells with IC₅₀ of 0.8 nM in HCT-116 cell line cytotoxicity assay^[1]. Epothilone B (Patupilone) is a microtubule (MT) targeting agent. As shown by MTT cell proliferation assay, after 72 h of treatment Epothilone B efficiently inhibits cell growth with an IC₅₀ of 6 nM, while concentrations ≤1 nM are not cytotoxic. Epothilone B significantly inhibits transwell cell migration at the non-cytotoxic concentration of 1 nM, and the effect is more evident at 10 nM^[2]. Epothilone B (Patupilone) is a novel, non-taxane-related and nonneurotoxic microtubule-stabilizing agent in human medulloblastoma cell lines. Epothilone B reduces the proliferative activity in the D341 cell line, with an IC₅₀ of 0.53 nM; in the D425Med cell line, with an IC₅₀ of 0.37 nM; and in the DAOY cell line, with an IC₅₀ of 0.19 nM. In the D341Med cell line, the effect of Epothilone B on clonogenic survival is at dose range of Epothilone B similar to the level of proliferative activity and viability (IC₅₀, 0.50-0.75 nM). However, the clonogenicity of D425Med and DAOY cells is already strongly reduced at a 10-fold lower concentration of Epothilone B (IC₅₀, 30 pM). These results overall demonstrate that Epothilone B is highly potent against different medulloblastoma cell lines^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Treatment with Epothilone B (Patupilone) or ionizing radiation alone results in a partial tumor growth suppression over 10 days, whereas combined treatment exerts a strong supra-additive tumor growth control, with complete tumor regression in the follow-up period (P<0.005, for ionizing radiation or Epothilone B alone vs combined treatment)^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 152044-54-7
Appearance Solid
Molecular Weight 507.68
Formula C₂₇H₄₁NO₆S
Color White to off-white
SMILES

O=C(C[C@H](O)C1(C)C)O[C@H](/C(C)=C/C2=CSC(C)=N2)C[C@]3([H])O[C@]3(C)CCC[C@H](C)[C@H](O)[C@@H](C)C1=O
Synonyms

EPO 906; Patupilone
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (8)

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J Biomed Sci

CASK promotes non-small cell lung cancer growth through coordinated regulation of EGFR expression, trafficking, and p21 expression. [Abstract]

2026 May 9;33(1):49. PMID: 42106797
Eur J Pharm Sci

Epothilone B inactivation of Sirtuin1 promotes mitochondrial reactive oxygen species to induce dysfunction and ferroptosis of Schwann cells. [Abstract]

2023 Feb 1:181:106350. PMID: 36496165
Am J Pathol

Selective and Marked Blockade of Endothelial Sprouting Behavior Using Paclitaxel and Related Pharmacologic Agents. [Abstract]

2021 Dec;191(12):2245-2264. PMID: 34563512
Biochem Biophys Res Commun

High-resolution X-ray structure of three microtubule-stabilizing agents in complex with tubulin provide a rationale for drug design. [Abstract]

2021 Jan 1;534:330-336. PMID: 33272565
McGill University

2025.
bioRxiv

2025 Nov 12.
bioRxiv

2021 Feb 5.
Oncotarget

Molecular-genetic profiling and high-throughput in vitro drug screening in NUT midline carcinoma-an aggressive and fatal disease. [Abstract]

2017 Dec 2;8(68):112313-112329. PMID: 29348827

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (196.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.9697 mL

9.8487 mL

19.6974 mL

5 mM

0.3939 mL

1.9697 mL

3.9395 mL

10 mM

0.1970 mL

0.9849 mL

1.9697 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (4.10 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.08 mg/mL (4.10 mM); Clear solution; Need warming

This protocol yields a clear solution of 2.08 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Kinase Assay^[3]

Asp-Glu-Val-Asp (DEVD)ase activity is determined in cytosolic cell extracts. Cells are treated with increasing concentrations of Epothilone B (Patupilone) for 6, 12, 24, and 48 h. Cells are harvested thereafter by trypsin/EDTA, centrifuged, and washed with precooled PBS. The cell pellet is suspended in 5 volumes of precooled buffer A (20 mM HEPES-KOH [pH 7.5], 10 mM KCl, 1.5 mM MgCl₂, 1 mM sodium EDTA, 1 mM sodium EGTA, 1 mM dithiothreitol [DDT], 250 mM sucrose, and 0.1 mM phenylmethylsulfonyl fluoride [PMSF] supplemented with protease inhibitors [5 mg/mL pepstatin A, 10 mg/mL leupeptin, 2 mg/mL aprotinin, 2 mg/mL DTT, and 1 mM of PMSF]). After incubation on ice for 15 min, the cells are disrupted by freezing and thawing. Cell lysates are centrifuged at 1000g for 10 min at 4°C, and the supernatant is further centrifuged at 100 000g for 30 min. The resulting supernatant (S-100 fraction) is stored at −80°C. To determine caspase 3-like activity, 75 μg of protein from the S-100 fraction is incubated at 37°C with the colorimetric caspase 3 substrate N-acetyl-Asp-Glu-Val-Asp p-nitroanilide (100 mM; Ac-DEVD-pNA) and 1 mM dATP in a final volume of 120 μL. Cleavage of the caspase substrate is monitored at 405 nm using a GenTec spectrophotometer^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Assay^[2]

Human glioblastoma cells (U87MG, ATCC) are routinely maintained at 37°C and 5% CO₂ in EMEM medium, with NEAA, containing 10% fetal bovine serum, 2 mM of glutamine, 1% penicillin and streptomycin. U87MG cells are used for no more than 15 passages. Cells are seeded in 96-well plates (5000 cells/well). After 24 h cells are treated with Epothilone B. Growth inhibition of U87MG cells is measured after 72 h of drug treatment by using the MTT cell proliferation assay^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[3]

Mice^[3]
D425Med cells (6×10⁶) are injected subcutaneously on the backs of 4-6-week-old athymic nude mice. Tumor volumes are determined from caliper measurements of tumor length (L) and width (l) according to the formula (L×l²)/2. Tumors are allowed to expand to a volume of 200 mm³ (±10%) before treatment start. With the use of a customized shielding device, mice are given strictly loco regional radiotherapy of 3×3 Gy on 3 consecutive days using a Gulmay 200 kV X-ray unit at 100 cGy/min at room temperature. Epothilone B (2 mg/kg; dissolved in 30% PEG-300/70% saline) is applied intravenously 24 h before the first treatment with ionizing radiation (at day 0 of the treatment; n=5 per group). Tumor growth is monitored daily.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Regueiro-Ren A, et al. Synthesis and biological activity of novel epothilone aziridines. Org Lett. 2001 Aug 23;3(17):2693-6. [Content Brief]

[2]. Pagano A, et al. Epothilone B inhibits migration of glioblastoma cells by inducing microtubule catastrophes and affecting EB1 accumulation at microtubule plus ends. Biochem Pharmacol. 2012 Aug 15;84(4):432-43. [Content Brief]

[3]. Oehler C, et al. The microtubule stabilizer patupilone (epothilone B) is a potent radiosensitizer in medulloblastoma cells. Neuro Oncol. 2011 Sep;13(9):1000-10. [Content Brief]

[4]. Jang EH, et al. Effects of Microtubule Stabilization by Epothilone B Depend on the Type and Age of Neurons. Neural Plast. 2016;2016:5056418. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.9697 mL	9.8487 mL	19.6974 mL	49.2436 mL
	5 mM	0.3939 mL	1.9697 mL	3.9395 mL	9.8487 mL
	10 mM	0.1970 mL	0.9849 mL	1.9697 mL	4.9244 mL
	15 mM	0.1313 mL	0.6566 mL	1.3132 mL	3.2829 mL
	20 mM	0.0985 mL	0.4924 mL	0.9849 mL	2.4622 mL
	25 mM	0.0788 mL	0.3939 mL	0.7879 mL	1.9697 mL
	30 mM	0.0657 mL	0.3283 mL	0.6566 mL	1.6415 mL
	40 mM	0.0492 mL	0.2462 mL	0.4924 mL	1.2311 mL
	50 mM	0.0394 mL	0.1970 mL	0.3939 mL	0.9849 mL
	60 mM	0.0328 mL	0.1641 mL	0.3283 mL	0.8207 mL
	80 mM	0.0246 mL	0.1231 mL	0.2462 mL	0.6155 mL
	100 mM	0.0197 mL	0.0985 mL	0.1970 mL	0.4924 mL

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Epothilone B

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Chemical Information

Publications (8)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Epothilone B Related Classifications

Cat. No.: HY-17029