Galangin (Synonyms: Norizalpinin; 3,5,7-Trihydroxyflavone)

Name: Galangin
Brand: MedChemExpress
SKU: HY-N0382
Rating: 5.0 (18 reviews)

Cat. No.: HY-N0382 Purity: 99.99%: Data Sheet
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Galangin (Norizalpinin) is an agonist/antagonist of the arylhydrocarbon receptor. Galangin (Norizalpinin) also shows inhibition of CYP1A1 activity.

For research use only. We do not sell to patients.

CAS No. : 548-83-4

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 18 publication(s) in Google Scholar

Other Forms of Galangin:

Galangin-¹³C₃ Get quote
Galangin (Standard) Get quote

18 Publications Citing Use of MCE Galangin

Bio/Physico-chemical Assay

Histological Imaging/Staining

Cell Imaging/Staining

In Vivo Efficacy Study

: Galangin purchased from MedChemExpress. Usage Cited in: Microbiome. 2025 Jun 18;13(1):145. [Abstract]; Fluorescence microscopy viewing the attachment of PTCs to endometrial microfluidic chip. Galangin (5 µM; 24 h) could markedly motivate the adhesion of PTCs to endometrial epithelium.

: Galangin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Aug 7. [Abstract]; Galangin-induced outward currents were normalized to the maximal effect (Emax) value and fitted to a logistic function (n = 6).

: Galangin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Aug 7. [Abstract]; Galangin (1.8–30 mg/kg, i.p.) significantly elevated mechanical and thermal pain thresholds in SNI mice in a dose-dependent manner within 1–5 hours after administration.

: Galangin purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2025 Aug 7. [Abstract]; The reduction in mechanical allodynia induced by Galangin (15 mg/kg; i.p.) was reversed by the blocker XE991 (3 mg/kg).

: Galangin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2023 Aug 22:17:2495-2511.; Galangin (Gal, 15 mg/kg; i.p.; 4 days) attenuated myocardial injury after IR in in vivo study. Representative TTC-Evans Blue stained sections of hearts from each group. The non-risk area stained by Evans Blue is indicated in blue, the area at risk (AAR) stained by TTC is indicated in red and white, whereas the unstained (white) area represents the infarcted area (IA). The results revealed a markedly reduced IA/AAR in the IR + Gal group compared with that in the IR group.

: Galangin purchased from MedChemExpress. Usage Cited in: Drug Des Devel Ther. 2023 Aug 22:17:2495-2511.; Galangin (Gal, 15 mg/kg; i.p.;4 days) reversed the downregulation of anti-ferroptotic proteins Gpx4 and FTH induced by IR injury in mice.

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View All Cytochrome P450 Isoform Specific Products:

View All Isoforms

CYP1 CYP2 CYP3 CYP4 CYP11 CYP17 Aromatase/CYP19A1 CYP26 CYP51 CYP1A2 CYP2D6 CYP2C9 CYP2C19 CYP3A CYP3A4 CYP17A1

Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Galangin (Norizalpinin) is an agonist/antagonist of the arylhydrocarbon receptor. Galangin (Norizalpinin) also shows inhibition of CYP1A1 activity.

IC₅₀ & Target

CYP1

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	39.45 μg/mL Compound: 6	Cytotoxicity against human A431 cells Cytotoxicity against human A431 cells	[PMID: 19027298]
A549	EC₅₀	93.6 μM Compound: 4	Antiproliferative activity against human A549 cells after 72 hrs by MTT assay Antiproliferative activity against human A549 cells after 72 hrs by MTT assay	[PMID: 12027739]
A549	IC₅₀	54.21 μg/mL Compound: 6	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 19027298]
B16	IC₅₀	17 μM Compound: 6	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs	[PMID: 19615910]
B16-4A5	IC₅₀	100 μM Compound: 6	Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay Inhibition of theophylline-stimulated mouse B16-4A5 cell proliferation assessed as cell viability after 68 hrs by WST8 assay	[PMID: 19615910]
B16-BL6	EC₅₀	26.8 μM Compound: 4	Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay	[PMID: 12027739]
B16-F10	IC₅₀	> 200 μM Compound: 2	Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay Cytotoxicity against mouse B16-F10 cells assessed as reduction in cell viability incubated for 72 hrs by XTT assay	[PMID: 27720556]
CHO	IC₅₀	26.96 μg/mL Compound: 6	Cytotoxicity against CHO cells Cytotoxicity against CHO cells	[PMID: 19027298]
COLO 205	IC₅₀	33.14 μg/mL Compound: 6	Cytotoxicity against human COLO205 cells Cytotoxicity against human COLO205 cells	[PMID: 19027298]
H9	EC₅₀	28 μM Compound: 18	Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay Antiviral activity against HIV1 3B infected in human H9 cells assessed as inhibition of viral replication after 3 days by p24 antigen capture assay	[PMID: 8158164]
H9	IC₅₀	44 μM Compound: 18	Cytotoxicity against human H9 cells after 3 days Cytotoxicity against human H9 cells after 3 days	[PMID: 8158164]
HEK293	IC₅₀	0.45 μM Compound: 19	Inhibition of human TRPC5 expressed in HEK293 cells assessed as reduction in gadolinium-induced calcium entry after 30 mins by fluo-4 dye based fluorescence assay Inhibition of human TRPC5 expressed in HEK293 cells assessed as reduction in gadolinium-induced calcium entry after 30 mins by fluo-4 dye based fluorescence assay	[PMID: 30943030]
HT-1080	EC₅₀	35.7 μM Compound: 4	Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	EC₅₀	79 μM Compound: 4	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 12027739]
HeLa	IC₅₀	>= 10 μM Compound: galangin	Cytotoxicity against human HeLa cells by MTT assay Cytotoxicity against human HeLa cells by MTT assay	[PMID: 19278239]
Huh-7	CC₅₀	> 50 μM Compound: 29	Cytotoxicity against human Huh7.5.1 cells by MTT assay Cytotoxicity against human Huh7.5.1 cells by MTT assay	[PMID: 22445328]
KB	ED₅₀	> 100 μg/mL Compound: NSC-407229	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 469554]
L929	EC₅₀	40 μM Compound: galangin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by crystal violet staining	[PMID: 9287415]
L929	EC₅₀	50 μM Compound: galangin	Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay Inhibition of recombinant human TNF-alpha-induced cytotoxicity of mouse L929 cells assessed as survivality preincubated for 15 mins before TNFalpha addition measured after 24 hrs by [methyl-3H]thymidine incorporation assay	[PMID: 9287415]
MCF7	IC₅₀	4.2 μg/mL Compound: Galangin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 3 days by beta scintillation counting based [3H]-thymidine incorporation assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 3 days by beta scintillation counting based [3H]-thymidine incorporation assay	[PMID: 33257172]
MCF7	IC₅₀	53.08 μg/mL Compound: 6	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 19027298]
Vero	CC₅₀	43.2 μg/mL Compound: galangin	Cytotoxicity against african green monkey Vero cells Cytotoxicity against african green monkey Vero cells	[PMID: 1338212]

In Vitro

Galangin (Norizalpinin)?inhibits the catabolic breakdown of DMBA, as measured by thin-layer chromatography, in a dose-dependent manner.?Galangin?also inhibits the formation of DMBA-DNA adducts, and prevents DMBA-induced inhibition of cell growth.?Galangin?causes a potent, dose-dependent inhibition of?CYP1A1?activity, as measured by ethoxyresorufin-O-deethylase?activity, in intact cells and in microsomes isolated from DMBA-treated cells. Analysis of the inhibition kinetics by double-reciprocal plot demonstrates that?galangin?inhibits CYP1A1?activity?in a noncompetitive manner.?Galangin?causes an increase in the level of?CYP1A1?mRNA, indicating that it may be an?agonist?of the?aryl?hydrocarbon?receptor, but it inhibits the induction of?CYP1A1?mRNA by DMBA or by 2,3,5,7-tetrachlorodibenzo-p-dioxin (TCDD).?Galangin?also inhibits the DMBA- or TCDD-induced transcription of a reporter vector containing the?CYP1A1?promoter^[1]. Galangin treatment inhibits cell proliferation and induced autophagy (130 μM) and apoptosis (370 μM). In particular, galangin treatment in HepG2 cells causes (1) an accumulation of autophagosomes, (2) elevated levels of microtubule-associated protein light chain 3, and (3) an increased percentage of cells with vacuoles. p53 expression is also increased. The galangin-induced autophagy is attenuated by the inhibition of p53 in HepG2 cells, and overexpression of p53 in Hep3B cells restored the galangin-induced higher percentage of cells with vacuoles to normal level^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

270.24

Formula

C₁₅H₁₀O₅

CAS No.

548-83-4

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C1C(O)=C(C2=CC=CC=C2)OC3=CC(O)=CC(O)=C13

Structure Classification

Initial Source

Microorganisms

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (462.55 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.7004 mL	18.5021 mL	37.0041 mL
5 mM	0.7401 mL	3.7004 mL	7.4008 mL
10 mM	0.3700 mL	1.8502 mL	3.7004 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (7.70 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.99%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (250 KB) HNMR (274 KB) RP-HPLC (265 KB) MS (68 KB)

Handling Instructions (2659 KB)

References

[1]. Ciolino HP, et al. The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor. Br J Cancer. 1999 Mar;79(9-10):1340-6. [Content Brief]

[2]. Wen M, et al. Galangin induces autophagy through upregulation of p53 in HepG2 cells. Pharmacology. 2012;89(5-6):247-55. [Content Brief]

Cell Assay
^[2]

Cells (5.0×10³) are seeded and treated with different concentrations of galangin for different periods of time in 96-well plates. The number of viable cells in each well is determined by adding 10 µL of 5 mg/mL MTT solution. Following the 4 hour incubation at 37°C, the cells are dissolved in a 100 µL solution containing 20% SDS and 50% dimethy formamide. The optical densities are quantified at a test wavelength of 570 nm with a reference wavelength of 630 nm using a Varioskan Flash Reader spectrophotometer.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

[1]. Ciolino HP, et al. The flavonoid galangin is an inhibitor of CYP1A1 activity and an agonist/antagonist of the aryl hydrocarbon receptor. Br J Cancer. 1999 Mar;79(9-10):1340-6. [Content Brief]

[2]. Wen M, et al. Galangin induces autophagy through upregulation of p53 in HepG2 cells. Pharmacology. 2012;89(5-6):247-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.7004 mL	18.5021 mL	37.0041 mL	92.5104 mL
	5 mM	0.7401 mL	3.7004 mL	7.4008 mL	18.5021 mL
	10 mM	0.3700 mL	1.8502 mL	3.7004 mL	9.2510 mL
	15 mM	0.2467 mL	1.2335 mL	2.4669 mL	6.1674 mL
	20 mM	0.1850 mL	0.9251 mL	1.8502 mL	4.6255 mL
	25 mM	0.1480 mL	0.7401 mL	1.4802 mL	3.7004 mL
	30 mM	0.1233 mL	0.6167 mL	1.2335 mL	3.0837 mL
	40 mM	0.0925 mL	0.4626 mL	0.9251 mL	2.3128 mL
	50 mM	0.0740 mL	0.3700 mL	0.7401 mL	1.8502 mL
	60 mM	0.0617 mL	0.3084 mL	0.6167 mL	1.5418 mL
	80 mM	0.0463 mL	0.2313 mL	0.4626 mL	1.1564 mL
	100 mM	0.0370 mL	0.1850 mL	0.3700 mL	0.9251 mL

Galangin Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Galangin548-83-4Norizalpinin 3,5,7-TrihydroxyflavoneCytochrome P450AutophagyCYPsInhibitorinhibitorinhibit

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Galangin (Synonyms: Norizalpinin; 3,5,7-Trihydroxyflavone)

Customer Review

Other Forms of Galangin:

Galangin Related Antibodies

18 Publications Citing Use of MCE Galangin

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Biological Activity

Protocol

Purity & Documentation

References

Customer Review

Galangin Related Antibodies

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Complete Stock Solution Preparation Table

Galangin Related Classifications

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