1. Membrane Transporter/Ion Channel
  2. TRP Channel

ML204 

Cat. No.: HY-12949 Purity: 98.31%
Handling Instructions

ML204 is a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels.

For research use only. We do not sell to patients.
ML204 Chemical Structure

ML204 Chemical Structure

CAS No. : 5465-86-1

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 92 In-stock
5 mg USD 84 In-stock
10 mg USD 120 In-stock
50 mg USD 384 In-stock
100 mg USD 624 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Other Forms of ML204:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

ML204 is a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. IC50 value: Target: TRPC4/C5 inhibitor ML204 inhibited TRPC4β-mediated intracellular Ca(2+) rise with an IC(50) value of 0.96 μm and exhibited 19-fold selectivity against muscarinic receptor-coupled TRPC6 channel activation. In whole-cell patch clamp recordings, ML204 blocked TRPC4β currents activated through either μ-opioid receptor stimulation or intracellular dialysis of guanosine 5'-3-O-(thio)triphosphate (GTPγS), suggesting a direct interaction of ML204 with TRPC4 channels rather than any interference with the signal transduction pathways. Selectivity studies showed no appreciable block by 10-20 μm ML204 of TRPV1, TRPV3, TRPA1, and TRPM8, as well as KCNQ2 and native voltage-gated sodium, potassium, and calcium channels in mouse dorsal root ganglion neurons. In isolated guinea pig ileal myocytes, ML204 blocked muscarinic cation currents activated by bath application of carbachol or intracellular infusion of GTPγS, demonstrating its effectiveness on native TRPC4 currents [1]. ML204 blocked TRPC4 channels in an electrophysiological assay with an IC value of 2.6 μM and was also active in fluorescent and electrophysiological assays in which TRPC4 channels were activated by different mechanisms, indicating direct block of TRPC4 channels. Selectivity for block of TRPC4 channels was examined in fluorescent and electrophysiological experiments against closely related TRPC channels and more distantly related TRPV, TRPA and TRPM channels, and against non-TRP ion channels. ML204 afforded good selectivity (19-fold) against TRPC6 channels and more modest selectivity against TRPC3 and TRPC5 (9-fold) channels [2].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 4.4185 mL 22.0926 mL 44.1852 mL
5 mM 0.8837 mL 4.4185 mL 8.8370 mL
10 mM 0.4419 mL 2.2093 mL 4.4185 mL
Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

226.32

Formula

C₁₅H₁₈N₂

CAS No.

5465-86-1

SMILES

CC1=CC(N2CCCCC2)=NC3=CC=CC=C13

Storage
Pure form -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 37 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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Product Name:
ML204
Cat. No.:
HY-12949
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