ML204 hydrochloride
Based on 8 publication(s) in Google Scholar
ML204 hydrochloride is a novel, potent, selective TRPC4/TRPC5 channel inhibitor, with at least 19-fold selectivity against TRPC6 and no appreciable effect on all other TRP channels, nor on voltage-gated sodium, potassium, or Ca2+ channels.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 2070015-10-8
- Formula: C15H19ClN2
- Molecular Weight:262.78
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
-
Membrane Transporter/Ion Channel Compound Library
HY-L011
-
Neuronal Signaling Compound Library
HY-L013
-
Mechanoreceptors Compound Library
HY-L095
-
Heterocyclic Compound Library
HY-L138
-
Pain-Related Compound Library
HY-L139
-
Membrane Protein-targeted Compound Library
HY-L149
-
Membrane Receptor-targeted Compound Library
HY-L150
-
Highly Selective Inhibitors Library
HY-L158
-
Ion Channel Compound Library
HY-L166
-
Multi-Target Compound Library
HY-L176
-
Bioactive Compound Library Max
HY-L181
-
MCE Bioactive Compound Library
HY-L001V
-
Bioactive Compound Library
HY-L001
-
High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) ML204 hydrochloride
More- Int J Biol Sci. 2024 Sep 16;20(12):4922-4940. [Abstract]
- J Transl Med. 2025 Nov 10;23(1):1254. [Abstract]
- J Ethnopharmacol. 2022 May 23:290:115105. [Abstract]
- Neuropharmacology. 2024 Jul 1:252:109946. [Abstract]
- Exp Cell Res. 2022 Dec 1;421(1):113374. [Abstract]
- Neurosci Res. 2024 Feb:199:48-56. [Abstract]
- Biochem Biophys Res Commun. 2025 Apr 1:755:151566. [Abstract]
- Biol Pharm Bull. 2023;46(6):864-868. [Abstract]
Biological Activity
|
TRPC4 |
TRPC5 |
ML204 hydrochloride inhibits TRPC4β-mediated intracellular Ca2+ rise with an IC50 value of 0.96 μM (HEK293 cells) and exhibits 19-fold selectivity against muscarinic receptor-coupled TRPC6 channel activation[1].
ML204 hydrochloride blocks TRPC4β activity induced through either Gi/o stimulation by μ-opioid, 5HT1A serotonin, and M2 muscarinic receptors or Gq/11 stimulation by the endogenous M3-like muscarinic receptors[1].
ML204 hydrochloride blocks LPS-induced TRPC5 channel activity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Nonfasted male C57BL/6 (2-3 months)[4]
-
Dosage:1 mg/kg
-
Administration:Subcutaneous injection, twice a day, for 5 days (prior to LPS injection)
-
Result:Induces mortality associated with increased hypothermia in mice with LPS-induced systemic inflammatory response.
Chemical Information
-
CAS No. 2070015-10-8
-
Appearance Solid
-
Molecular Weight 262.78
-
Formula C15H19ClN2
-
Color White to off-white
-
SMILES
CC1=CC(N2CCCCC2)=NC3=CC=CC=C13.Cl[H]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (8)
-
Journal Impact Factor
-
Most Recent
-
Int J Biol Sci
Pharmacological manipulation of TRPC5 by kaempferol attenuates metastasis of gastrointestinal cancer via inhibiting calcium involved in the formation of filopodia. [Abstract]2024 Sep 16;20(12):4922-4940. PMID: 39309444 -
J Transl Med
Peroxynitrite regulates ER stress-mediated Ca2+ flux to mitochondria characterizing cardiac microvascular ischemia-reperfusion injury associated with hyperhomocysteinemia. [Abstract]2025 Nov 10;23(1):1254. PMID: 41214689 -
J Ethnopharmacol
Magnolol and honokiol target TRPC4 to regulate extracellular calcium influx and relax intestinal smooth muscle. [Abstract]2022 May 23:290:115105. PMID: 35157953 -
Neuropharmacology
GLP-1 modulated the firing activity of nigral dopaminergic neurons in both normal and parkinsonian mice. [Abstract]2024 Jul 1:252:109946. PMID: 38599494 -
Exp Cell Res
Inhibition of TRPC6 suppressed TGFβ-induced fibroblast-myofibroblast transdifferentiation in renal interstitial NRK-49F cells. [Abstract]2022 Dec 1;421(1):113374. PMID: 36206825 -
Neurosci Res
Exendin-4 increases the firing activity of hippocampal CA1 neurons through TRPC4/5 channels. [Abstract]2024 Feb:199:48-56. PMID: 37595875 -
Biochem Biophys Res Commun
TRPC1 downregulation enhances catecholamine secretion in adrenal chromaffin cells under metabolic syndrome conditions. [Abstract]2025 Apr 1:755:151566. PMID: 40043610 -
Biol Pharm Bull
Inhibition of TRPC4/5 Channel Is Effective in Protecting against Histamine-Induced Hyperpermeability by Blocking Ca2+ Influx in Lung Microvascular Endothelial Cells. [Abstract]2023;46(6):864-868. PMID: 37258152
Solvent & Solubility
H2O : ≥ 30 mg/mL (114.16 mM)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (297 KB)
-
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
-
Handling Instructions (2659 KB)
References
[1]. Miller M, et al. Identification of ML204, a novel potent antagonist that selectively modulates native TRPC4/C5 ion channels. J Biol Chem. 2011 Sep 23;286(38):33436-46. [Content Brief]
[2]. Miller MR, et al. Novel Chemical Inhibitor of TRPC4 Channels. Probe Reports from the NIH Molecular Libraries Program. [Content Brief]
[3]. Thomas Schaldecker, et al. Inhibition of the TRPC5 ion channel protects the kidney filter. J Clin Invest. 2013 Dec 2; 123(12): 5298–5309. [Content Brief]
[4]. Domingos M S Pereira, et al. Transient Receptor Potential Canonical Channels 4 and 5 Mediate Escherichia coli-Derived Thioredoxin Effects in Lipopolysaccharide-Injected Mice. Oxid Med Cell Longev. 2018 Jun 10;2018:4904696. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 3.8055 mL | 19.0273 mL | 38.0546 mL | 95.1366 mL |
| 5 mM | 0.7611 mL | 3.8055 mL | 7.6109 mL | 19.0273 mL | |
| 10 mM | 0.3805 mL | 1.9027 mL | 3.8055 mL | 9.5137 mL | |
| 15 mM | 0.2537 mL | 1.2685 mL | 2.5370 mL | 6.3424 mL | |
| 20 mM | 0.1903 mL | 0.9514 mL | 1.9027 mL | 4.7568 mL | |
| 25 mM | 0.1522 mL | 0.7611 mL | 1.5222 mL | 3.8055 mL | |
| 30 mM | 0.1268 mL | 0.6342 mL | 1.2685 mL | 3.1712 mL | |
| 40 mM | 0.0951 mL | 0.4757 mL | 0.9514 mL | 2.3784 mL | |
| 50 mM | 0.0761 mL | 0.3805 mL | 0.7611 mL | 1.9027 mL | |
| 60 mM | 0.0634 mL | 0.3171 mL | 0.6342 mL | 1.5856 mL | |
| 80 mM | 0.0476 mL | 0.2378 mL | 0.4757 mL | 1.1892 mL | |
| 100 mM | 0.0381 mL | 0.1903 mL | 0.3805 mL | 0.9514 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
ML204 hydrochloride Related Classifications
HY-12949A Related Classifications
Powered by Bioz