Methscopolamine bromide (Synonyms: (-)-Scopolamine methyl bromide; Hyoscine methyl bromide)

Name: Methscopolamine bromide
Brand: MedChemExpress
SKU: HY-B0344
Rating: 4.5 (1 reviews)

Cat. No.: HY-B0344 Purity: 99.53%: Data Sheet
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Methscopolamine bromide ((-)-Scopolamine methyl bromide; Hyoscine methyl bromide) is a peripherally acting muscarinic receptor (mAChR) antagonist that cannot cross the blood-brain barrier. Methscopolamine bromide blocks the muscarinic negative feedback regulation of acetylcholine release from striatal cholinergic terminals, thereby increasing acetylcholine release in the striatum of freely moving rats. Methscopolamine bromide does not induce motor excitation in freely moving rats, nor does it alter the duration of ethanol-induced loss of righting reflex in mice. Methscopolamine bromide fails to antagonize the arecoline-mediated reduction in the duration of ethanol-induced loss of righting reflex in mice.

For research use only. We do not sell to patients.

Methscopolamine bromide Chemical Structure

CAS No. : 155-41-9

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	Get quote
5 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Methscopolamine bromide

•Oncol Lett. 2016 Sep;12(3):1783-1791. [Abstract]

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mAChR1 mAChR2 mAChR3 mAChR4 mAChR5

Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vivo

Methscopolamine (0.25-1.0 mg/kg; subcutaneous injection; single dose) cannot antagonize the shortening effect of Arecoline (HY-N2364) on ethanol-induced hypnotic duration in mice, as it fails to cross the blood-brain barrier^[1].
Methscopolamine bromide (5-10 mg/kg; i.p.; single administration) maximally increases acetylcholine release in the striatum of freely moving rats by 380% and 430%, respectively, without altering their spontaneous motor activity^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	ICR mice (male, 18 to 22 g)^[1]
Dosage:	0.25 mg/kg; 1.0 mg/kg
Administration:	s.c.; single dose
Result:	Did not significantly alter the duration of ethanol-induced loss of the righting reflex (LORR) compared to saline-treated controls when administered alone. Did not reverse the arecoline-induced shortening of ethanol-induced LORR duration (no significant difference compared to the saline + arecoline group) when administered before arecoline.

Animal Model:	Wistar rats (male, 280-350 g)^[2]
Dosage:	5 mg/kg; 10 mg/kg
Administration:	i.p.; single injection
Result:	Caused a maximal 380% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours. Caused a maximal 430% increase in striatal acetylcholine release relative to baseline levels at 2 hours post-injection, with release remaining above baseline for over 4 hours. Did not cause a change in spontaneous motor activity relative to controls.

Molecular Weight

398.29

Formula

C₁₈H₂₄BrNO₄

CAS No.

155-41-9

Appearance

Solid

Color

White to off-white

SMILES

C[N+]1([C@@H]2[C@H](O3)[C@H]3[C@H]1C[C@@H](OC([C@@H](C4=CC=CC=C4)CO)=O)C2)C.[Br-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 50 mg/mL (125.54 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 50 mg/mL (125.54 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5107 mL	12.5537 mL	25.1073 mL
5 mM	0.5021 mL	2.5107 mL	5.0215 mL
10 mM	0.2511 mL	1.2554 mL	2.5107 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.28 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.81%

Select Batch:

Data Sheet (279 KB) SDS (537 KB)

COA (255 KB) LCMS (87 KB)

Handling Instructions (2659 KB)

References

[1]. Sun YP, et al. Systemic administration of arecoline reduces ethanol-induced sleeping through activation of central muscarinic receptor in mice. Alcohol Clin Exp Res. 2010;34(1):150-157.

[2]. Watanabe H, et al. Effect of anticholinergic drugs on striatal acetylcholine release and motor activity in freely moving rats studied by brain microdialysis. Jpn J Pharmacol. 1989;51(1):75-82.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	2.5107 mL	12.5537 mL	25.1073 mL	62.7683 mL
	5 mM	0.5021 mL	2.5107 mL	5.0215 mL	12.5537 mL
	10 mM	0.2511 mL	1.2554 mL	2.5107 mL	6.2768 mL
	15 mM	0.1674 mL	0.8369 mL	1.6738 mL	4.1846 mL
	20 mM	0.1255 mL	0.6277 mL	1.2554 mL	3.1384 mL
	25 mM	0.1004 mL	0.5021 mL	1.0043 mL	2.5107 mL
	30 mM	0.0837 mL	0.4185 mL	0.8369 mL	2.0923 mL
	40 mM	0.0628 mL	0.3138 mL	0.6277 mL	1.5692 mL
	50 mM	0.0502 mL	0.2511 mL	0.5021 mL	1.2554 mL
	60 mM	0.0418 mL	0.2092 mL	0.4185 mL	1.0461 mL
	80 mM	0.0314 mL	0.1569 mL	0.3138 mL	0.7846 mL
	100 mM	0.0251 mL	0.1255 mL	0.2511 mL	0.6277 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Methscopolamine bromide Related Classifications

Neurological Disease

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methscopolamine155-41-9(-)-Scopolamine methylHyoscine methylmAChRMuscarinic acetylcholine receptorstriatal cholinergic terminalsblood-brain barriermicestriatummuscarinic receptor antagonistacetylcholinefreely moving ratsethanol-induced loss of righting reflexarecolinemuscarinic negative feedback controlInhibitorinhibitorinhibit

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Methscopolamine bromide (Synonyms: (-)-Scopolamine methyl bromide; Hyoscine methyl bromide)

Customer Review

Methscopolamine bromide Related Antibodies

1 Publications Citing Use of MCE Methscopolamine bromide

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All mAChR Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Methscopolamine bromide Related Classifications

Keywords:

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Customer Review

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